1393/3/26، جلد ۹، شماره ۲، صفحات -
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عنوان انگلیسی Emerging Intestinal Microsporidia Infection in HIV+/AIDS Patients in Iran: Microscopic and Molecular Detection
چکیده انگلیسی مقاله Abstract Background: Species of Microsporidia have been known as opportunistic obli­gate intracellular parasites particularly in immunocompromised patients. Enterocyto­zoon bieneusi is one of most prevalent intestinal microsporida parasites in HIV + /AIDS patients. In this study, intestinal microsporidia infection was deter­mined in HIV + /AIDS patients using microscopic and molecular methods. Methods: Stool samples were collected from HIV + /AIDS patients during 12 months. All of the stool specimens washed with PBS (pH: 7.5). Slim slides were prepared from each sample and were examined using light microscope with 1000X magnification. DNA extraction carried out in microscopic positive samples. DNA amplification and genus/species identification also performed by Nested-PCR and sequencing techniques. Results: From 81 stool samples, 25 were infected with microsporidia species and E. bieneusi were identified in all of positive samples. No Encephalitozoon spp . was identified in 81 collected samples using specific primers. Conclusion: E. bieneusi is the most prevalent intestinal microsporidia in immunocompromised patients of Iran. On the other hand, Nested-PCR using spe­cific primers for ssu rRNA gene is an appropriate molecular method for identifica­tion of E. bieneusi . Keywords: HIV + /AIDS patients, Iran, Enterocytozoon bieneusi , Encephalitozoon spp ., Nested-PCR
کلیدواژه‌های انگلیسی مقاله Keywords: HIV + /AIDS patients, Iran, Enterocytozoon bieneusi , Encephalitozoon spp ., Nested-PCR
نویسندگان مقاله 54537---54538---54539---54540---54541---54542---54543---
نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/viewArticle/1163
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