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1393/3/26، جلد ۹، شماره ۲، صفحات -
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژههای فارسی مقاله |
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| عنوان انگلیسی |
Isolation and Identification of Naegleria Species from Environ-men¬tal Water in Changchun, Northeastern China |
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| چکیده انگلیسی مقاله |
Abstract Background: Naegleria is a free-living amoeba, and pathogenic Naegleria may pose a health risk to people exposed to recreational water. Our objective in this study was to determine if there are pathogenic amoebae in environmental water samples from Changchun, Northeastern China. Methods: During July to September 2012, a total of 70 water samples were collected from Changchun, Northeastern China, and Naegleria was enriched by in vitro culture and detected by PCR using Naegleria genus-specific primers. Resulting PCR products were sequenced and phylogenetically analyzed to identify Naegleria species. Results: Naegleria was detected in 65 (92.9%) of 70 water samples. DNA sequence and phylogenetic analyses based on the internal transcribed spacer (ITS) rDNA sequences revealed four Naegleria species, including N. pagei (n = 24) and N. Australiensis (n = 18), N. clarki (n = 13) and N. gruberi (n = 10), in which N. australiensis is pathogenic to mice. But the pathogenic species N. fowleri was not detected. Conclusion: This is the first report on Naegleria species in Northeastern China, showing that almost all environmental water samples were contaminated with Naegleria , including N. pagei , N. Australiensis , N. clarki and N. gruberi , which should be considered a potential public health threat. Keywords: Naegleria , PCR, Identification, Changchun, China |
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| کلیدواژههای انگلیسی مقاله |
Keywords: Naegleria , PCR, Identification, Changchun, China |
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| نویسندگان مقاله |
54634---54635---54636---54637---54638--- |
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| نشانی اینترنتی |
http://ijpa.tums.ac.ir/index.php/ijpa/article/viewArticle/1177 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Articles |
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