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1392/3/25، جلد ۸، شماره ۲، صفحات -
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Gene Regulation of Pteridine Reductase 1 in Leishmania Promasti-gotes and Amastigotes Using a Full-Length Antisense Construct |
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| چکیده انگلیسی مقاله |
ABSTRACT Background: Pteridine metabolic pathway is unusual features of Leishmania , which is necessary for the growth of parasite. Leishmania has evolved a complex and versatile pteridine salvage network which has the ability of scavenging a wide area of the conjugated and unconjugated pteridines especially folate and biopterin. In this study, we focus on the inhibition of ptr1 gene expression. Methods: L. major ptr1 gene was cloned into pcDNA3 and digested using KpnI and BamHI. The gene was subcloned so that antisense will transcribe and called pcDNA-rPTR. Leishmania major was cultured and late logarithmic-phase promastigotes were harvested. The promastigotes were divided into two groups. One group was transfected with 50 μg of pcDNA-rPTR, whereas the other group was transfected with pcDNA3. Transfected cells were cultured and plated onto semi-solid media. Mouse pritonean macrophages were transfected using pcDNA-rPTR–tansfected promastigotes. Western blotting was performed on mouse transfected pritonean macrophages and extracts from transfected promastigotes of L. major using a L. major ptr1 antibody raised in rabbits. Results: The PTR1 protein was not expressed in pcDNA-rPTR– tansfected promastigotes and mouse macrophage transfected with pcDNA-rPTR– tansfected promastigotes. Conclusion: This approach might be used to study the pteridine salvage pathway in Leishmania or to assess the possibility of using gene expression inhibition in the treatment of leishmaniasis. Keywords: Pteridine reductase 1, Antisense, Leishmania, Gene regulation, Inhibition |
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| کلیدواژه‌های انگلیسی مقاله |
Keywords: Pteridine reductase 1, Antisense, Leishmania, Gene regulation, Inhibition |
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| نویسندگان مقاله |
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| نشانی اینترنتی |
http://ijpa.tums.ac.ir/index.php/ijpa/article/viewArticle/619 |
| فایل مقاله |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Articles |
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