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1392/3/25، جلد ۸، شماره ۲، صفحات -
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژههای فارسی مقاله |
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| عنوان انگلیسی |
Application of the Reverse Line Blot Assay for the Molecular Detec¬tion of Theileria and Babesia sp. in Sheep and Goat Blood Samples from Pakistan |
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| چکیده انگلیسی مقاله |
ABSTRACT Background: The present study was designed to detect the presence of tick-borne parasites ( Theileria and Babesia spp . ) in 196 blood samples collected from apparently healthy sheep and goats from two provinces, Punjab and Khyber Pukhtoon Khwa, in Pakistan. Methods: Reverse line blot (RLB) assay was applied for the parasitic detection by the amplification of hypervariable V4 region of the 18S ribosomal RNA (rRNA) gene . A membrane with covalently linked generic and species specific oligonucleotide probes was used for the hybridization of amplified PCR products. Results: Parasites were detected in 16% of the ruminant blood samples under study. Two Theileria species, T. lestoquardi and T. ovis , were identified in samples. 25, of the total 32, infected animals were from Khyber Pukhtoon Khwa. Conclusion : Sheep were more prone to tick borne haemoprotozans as 81% infected samples were sheep as compared to 19% goats ( P > 0.001). Risk factor analysis revealed that male ( P = 0.03), animals infested by ticks ( P = 0.03) and herd composed of sheep only ( P = 0.001) were more infected by blood parasites. Keywords : Sheep, Goat, Theileria ; Babesia , 18S rRNA gene, RLB |
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| کلیدواژههای انگلیسی مقاله |
Keywords : Sheep, Goat, Theileria ; Babesia , 18S rRNA gene, RLB |
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| نویسندگان مقاله |
54743---54744---54745---54746---54747---54748--- |
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| نشانی اینترنتی |
http://ijpa.tums.ac.ir/index.php/ijpa/article/viewArticle/632 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Articles |
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