1391/5/28، جلد ۷، شماره ۱، صفحات -

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عنوان انگلیسی Spatial Distribution and Molecular Identification of Leishmania Species from Endemic Foci of South-Eastern Iran
چکیده انگلیسی مقاله Background: Cutaneous leishmaniasis constitutes a major public health problem in many parts of the world including Iran. The primary objective of this study was to identify Leishmania spe­cies in endemic districts of Kerman Province, south-eastern Iran . Methods: This study was conducted by random sampling as cross- sectional descriptive between 2008 and 2010. Overall, 203 skin scraping smears were taken from the patients. Nested -PCR was performed to amplify variable minicircle fragments of Leishmania kDNA. Results: Bam was the most infected district (71.1%), followed by Kerman (14.7%), Jiroft (5.4%), Baft (2.7%), Sirjan (1.6%), Shahr-e Babak (1.5%) and others (3.0%).  L. tropica was the most common species identified (194 cases, 95.6%), while L. major was found in only 9 cases (4.4%). Of 203 identified patients, all species in Bam (l07 cases), Kerman (32 cases), Jiroft (l6 cases) and Shahr-e- Babak (l1 cases) were detected as L. tropica , whereas infected subjects in Baft and Sir­jan showed L. tropica or L. major . Characterization of Leishmania species resulted in generation of 750 bp and 560 bp fragments, corresponding to those of L. tropica and L. major , respectively. Conclusion: L. tropica is the main species (95.6%) caused ACL in endemic areas of Kerman Prov­ince; however L. major is present in low level (4.4%).
کلیدواژه‌های انگلیسی مقاله Cutaneous leishmaniasis, Leishmania species, Identification, Nested-PCR, Iran,

نویسندگان مقاله 54815---54816---54817---54818---54819---54820---54821---54822---54823---

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/viewArticle/384
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