| چکیده انگلیسی مقاله |
Assessing CD34 + cell viability and absolute counts, at different stages of processing allows for accurate quality control. Alterations in the cryopreservation or collection technique can be spotted in their effect on CD34 + cell viability and absolute counts. Products that are cryopreserved for lengthy periods, such as cord blood samples, can be assessed for quality using the single platform flow cytometry methodology 1, 2 . Flow cytometric analysis was performed on post freeze cord blood samples. Samples were analyzed, using the double-colour single platform variant of the ISHAGE guidelines with the addition of the viability dye 7-AAD. Prior to sample preparation, we ensured that the white blood cells (WBC) concentration was not greater than 30x10 9 WBC cells/L. In our study, we used multi-parameter flow cytometry to determine the absolute number and viability of 4000 cryopreserved cord blood samples stored in liquid phase nitrogen of -192 0 C. Cryopreservation and processing of autologous stem cells collection, assessing CD34 + cell viability and absolute counts, found to remain the same during periods of time. Measuring CD34 + cell viability allows us the quality of control assessments of stem cells processing |
