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1391/7/22، جلد ۴، شماره ۲، صفحات -
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژههای فارسی مقاله |
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| عنوان انگلیسی |
PCR-RFLP Is a Useful Tool to Distinguish between C. Dubliniensis and C. Albicans in Cancer Patients in Iran |
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| چکیده انگلیسی مقاله |
Introduction: Candida dubliniensis and C. albicans are very similar in morphology and phenotypic characteristics. Approximation of this yeast species has caused major problems in identifying these two correctly. Materials and Methods: To distinguish among sixty yeast clinical isolates from patients with cancer, polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) was done. Results: PCR-RFLP of the ITS region showed different patterns between Candida dubliniensis and C. albicans after digestion with enzymes BlnI. All of the Clinical isolates were diagnosed as C .albicans. The results were confirmed by sequencing and RAPD-PCR. Conclusion: PCR-RFLP would be a useful and applicable technique in clinical laboratories for discrimination of C. albicans and C. dubliniensis. |
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| کلیدواژههای انگلیسی مقاله |
PCR- RFLP, C .dubliniensis, C. albicans, BlnI, |
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| نویسندگان مقاله |
14624---14625---14626---14627--- |
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| نشانی اینترنتی |
http://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/viewArticle/243 |
| فایل مقاله |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Articles |
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