Background and Aim: Several biomaterials have been presented for regeneration of intraosseous defects and Bio-Oss is one of the most accepted materials in this field. Neo-Oss may be the first and only regenerative material made in Iran which few studies have been published about it. The aim of this study was the histologic evaluation of Neo-Oss in comparison with Bio-Oss in experimental rabbit calvarial defects. Materials and Methods: This was an interventional in vivo study. After elevating muccoperiosteal flap 18 standard defects (3×6mm) in frontal & parietal bone were made by round bur on the calvarium of 6 New Zealand rabbits. Defects were randomly assigned to one of the following treatments: 1-No graft (control), 2-Bio-Oss graft 3-Neo-Oss graft. After 4 weeks the animals were killed and histologic sections prepared. The studied variables consisted of inflammation, type of regenerated bone, thickness of bony trabeculation and foreign body reaction. Data were analyzed by Friedman test with p<0.05 as the limit of significance.

Results: The results showed that Bio-Oss was effective for bone regeneration. Inflammation (P= 0.026) and foreign body reaction (P= 0.002) in Neo-Oss was significantly more than Bio-Oss group. Thickness of terabecula in Neo-Oss group (66.7% thin) was significantly different from Bio-Oss (100% thick) and control group (50% thin 50% thick) (P= 0.006). In Neo-Oss group, granulation tissue containing giant cells was observed and the rate of resorption during 4 weeks was high.

Conclusion: Based on the results of this study, the healing capacity of Neo-Oss in comparison with Bio-Oss is not acceptable.