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1382/2/11، جلد ۱۶، شماره ۳، صفحات ۷۲-۷۸
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| عنوان فارسی |
اندازه گيری فعاليت آنزيم الاستاز |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
An evaluation on elastase enzyme activity in gingival crevicular fluid in periodontitis |
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| چکیده انگلیسی مقاله |
Statement of Problem: Changes in protein levels, host calls enzymes and inflammatory mediators in gingival crevicular Fluid (GCF) are considered as diagnostic indicators of Periodontitis.
Purpose: he aim of the present study was to measure the elastase enzyme activity in gingival crevicular Fluid among patients with periodontitis.
Material and Methods: In this study, 52 periodontitis patients (experimental group) and 51 healthy subjects without any gingival inflammatio (control group) were participated. Subjects of the periodontitis group showed pockets of 4-5 mm depth without gingival enlargement and recession or pockets of 1-2 mm depth with gingival recession. For enzyme activity measurement, lOOu,! of gingival fluid of each sample was mixed with lOOu! of enzyme substrate on the tube. The mixture was incubated at 34°c for lh with a buffer solution
of 1ml volume and absorbance was read at 410nm with spectrophotometer. The enzyme activity differences between two groups were analyzed by student t test.
Results: The elastase enzyme activity in gingival crevicular fluid in subjects with periodontium destruction and control subjects was 153±11.3 and 52.7±10.4 enzyme unit in ml per minute, respectively. The difference between groups was statistically significant (PO.05).
Conclusion: Based on the findings of this study, the measurement of elastae enzyme activity could be a useful indication of tissue changes that may ultimately manifest clinically as periodontitis. |
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| کلیدواژه‌های انگلیسی مقاله |
Gingival crevicular fluid,Elastase |
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| نویسندگان مقاله |
15767---15768---15769--- |
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| نشانی اینترنتی |
http://jdm.tums.ac.ir/browse.php?a_code=A-10-25-410&slc_lang=fa&sid=fa |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
general |
| نوع مقاله منتشر شده |
Research |
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