Background: To evaluate long-term outcome and satisfaction of MacIndo surgery with and without amnion graft.

Methods: This is a historical cohort study of patients who were treated with the McIndo procedure for vaginal agenesis. Participants answered a structured questionnaire to describe outcome in sexual function and satisfaction. Patient characteristics along with short and long-term endings were abstracted from the medical records.

Results: Thirty-five patients responded to the questionnaire. Average age (±SD) at surgery was 28.4 ± 3 years (range 17- 48 years). The mean number of years (±SD) since surgery was 7 years (range 2-12 years). In 15 patients (42.8%) amniotic graft were amniotic graft were used only in is patients (42.8%). There was no relation between two types of surgery (long-term outcome and satisfaction with p-values of 0.346 and 0.758 respectively).

Conclusions: Since there was not a statistical difference between these two types of McIndo methods, it is recommended to apply the procedure in which amnion is not used. This procedure (without amnion graft), is an easier method with less operation time and reduced viral (HIV…) transmission.