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1380/8/10، جلد ۵۹، شماره ۶، صفحات ۹۲-۹۷
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| عنوان فارسی |
بکارگيری کروماتوگرافی مايع با کارکرد بالا |
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| چکیده فارسی مقاله |
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| کلیدواژههای فارسی مقاله |
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| عنوان انگلیسی |
Application of hight performance liquid chromatography with spectrofluorimetric detection for determination of human milk retinol |
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| چکیده انگلیسی مقاله |
World health organization has introduced concentration of vitamin A in breast milk as a proper indicator for characterization of vitamin A deficiency in lactating mothers and their infants (<6 mo). A normal phase high performance liquid chromatography with spectrofluorometric detection as a specific and sensitive detector was used for characterization of all-trans retional from 13-Cis retinal. The method was sensitive (0.009 ppm) and accurate (1±0.054 µ mol/l) and had a good recovery percentage (99.36%). This method was more better than before methods and was compatible with the other useful methods. Effect of freezing and defreezing on all-trans retinol content of milk sample was investigated. The result was interesting, whatever all-trans retinol content was bigger, it’s destroying was bigger too. Because of that reseon, day today reproducibility wasn’t good. Standard of retinol was 95% all-trans and pure so we should use this method because two retinol isomers wasn’t separated from each other by reversed phase chromatography and UV detection. |
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| کلیدواژههای انگلیسی مقاله |
Determination ، All-trans retinol ، Specrofluoromemetric detection |
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| نویسندگان مقاله |
20933---20934--- |
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| نشانی اینترنتی |
http://tumj.tums.ac.ir/browse.php?a_code=A-10-25-5118&slc_lang=fa&sid=fa |
| فایل مقاله |
فایلی برای مقاله ذخیره نشده است |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
fa |
| موضوعات مقاله منتشر شده |
General |
| نوع مقاله منتشر شده |
Original Article |
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