1385/8/22، جلد ۱۴، شماره ۱، صفحات ۲۱-۲۷

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عنوان انگلیسی Determination of chemical and radiochemical purity of [14C] clozapine by high performance liquid chromatography and liquid scintillation [Persian]
چکیده انگلیسی مقاله Introduction: The mechanism of action and metabolism of clozapine, a complex drug used in psychopharmacology, is not completely understood. The effects of this compound in human body are related to some interactions with serotonin and dopamine neurotransmitter receptors. To further elucidate the mechanism of action and to support ongoing drug metabolism studies, [14C] clozapine with suitable radiochemical purity was synthesized in nuclear research center of AEOI. Methods: In this report radiochemical and chemical purity of [14C] clozapine were determined by liquid scintillation and reversed phase-HPLC respectively. Separation of [14C] clozapine and its synthesized interface products was carried on C8 optimal column and UV detector at 254nm.The mobile phase consisted of acetonitril-buffered aqueous solution of sodium phosphate with a flow rate of 1ml/min. Results: Detection limit and analytical precision of RP-HPLC method were 0.01µg/ml and 1-3.4% respectively. The chemical purity of synthesized clozapine was >99%. For determination of activity and radiochemical purity, liquid scintillation spectrometer was used and counting was done by direct addition of samples to ACS (Amersham) solution. Specific activity of synthesized clozapine was 10µCi/mg and radiochemical purity of >99. % was achieved. Conclusion: By separation of [14C] clozapine and its synthesized intermediate products the represented methods, in addition to determination of total yield of reaction, it was possible to determine every step of reaction therefore; it was not nessaseray to use classical methods such as: distillation, liquid-liquid extraction, etc. If the HPLC instrument is equipped with radiochemical detector, simultaneous determination of radiochemical as well as chemical yields of products are possible.
کلیدواژه‌های انگلیسی مقاله Iran, meta-analysis, trend of infertilityGated myocardial perfusion SPECT, Quantification, segmental scoring, Meckel’s scan, Meckel’s diverticulum, Gastrointestinal bleeding, Technetium Pertechnetate scintigraphy, Thiosemicarbazone, 191Os, Radiolabeling, Biodistribution, RBBB, Myocardial perfusion, Gated SPECT, Coronary artery disease, Effective dose, Nuclear medicine procedures, Collective effective dose, Tc99m MIBI parathyroid scintigraphy, Myocardium perfusion gated SPECT, Parathyroid adenoma, Skeletal scintigraphy, Fluid restriction, Tc-99m MDP, Image quality, Bone to soft tissue ratio, Radiology, Nuclear medicine, Medical imaging, Bibliometric study, Iran, Hyperthyroidism, Radioactive iodine, Sexual hormones, Semen analysis, Attenuation correction, Artifact, Color intensity, SPECT, Thyroid, I-131, Absorbed ratio, Monte-Carlo, Absorbed dose, MCNP-4A, Carcinoid Tumor, Liver metastases, ¹³¹I-MIBG scintigraphy, Quality Control, PET, 82mRb, 82Kr, Myocardial perfusion, Drug delivery systems, Gamma-scintigraphy, Neutron activation, Pharmacoscintigraphy, Post stroke dementia, Cerebral perfusion SPECT, ECD, 99mTc-DTPA, Renography, Deconvolution, Renal transit time parameters, Nuclear medicine, PET tracer, Gallium, Copper, Tc-94m, Minimally invasive radio-guided surgery, Hyperparathyroidism, Tc-99m sestamibi, Parathyroid adenoma, Parathyroid hyperplasia, Bombesin, 99mTc, Tumor, HYNIC, Radioiodine therapy, TLD, Thyroid cancer, Absorbed dose, Myocardial perfusion scan, Ischemic heart disease, Risk factors, Demographic data, Military hospital, Evidence based medicine, Nuclear medicine, Evidence based medicine, Nuclear medicine, Critical appraisal, Sensitivity, Specificity, Cavernous hemangioma, Radionuclide imaging, 99mTc-RBC, Attenuation correction, Myocardial perfusion, SPECT, Radio-iodine therapy, Liothyronine, Thermoluminescent dosimeter, Accumulated dose, Exposure rate, 106Ru eye plaque, Dose calculation, Mathematical human eye model, MCNP4C code, Sentinel lymph node, Tc-99m antimony sulfide colloid, Lymphoscintigraphy, Skin marking, Osteoporosis, Type 2 diabetes, Postmenopausal, BMD, Streptokinase, Gallium-67, Thrombosis, SPECT, Radiopharmaceuticals, Breast uptake, Myocardial perfusion scan, Tc-99m sestamibi, Tc-99m pertechnetate scan, Meckel’s diverticulum, Gastrointestinal bleeding, PET, Sensitivity, SPECT, Spatial resolution, Temporal resolution, Dynamic imaging, Attenuation, Collimator, Random coincidence, Animal imaging, Dual isotope imaging, Time-of-Flight, Artifact, Bone scan, Catheter, PSA, Prostate, RIA, Standard matrix, Human serum, Myocardial perfusion scan, Leptin, BMI, [14C]Clozapine, Radiochemical purity, High performance liquid chromatography, Liquid scintillation spectrometer,

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