1389/10/6، جلد ۱۸، شماره ۲، صفحات ۹-۱۹

عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله

عنوان انگلیسی New freeze-dried kit for diagnosis of Bombesin receptor expressing tumors
چکیده انگلیسی مقاله   Introduction: It has been shown that some primary human tumors and their metastases, including prostate and breast tumors, over-express gastrin-releasing peptide (GRP) receptors. Bombesin is a neuropeptide with a high affinity for these GRP receptors. The purpose of this study was to prepare and evaluate the characteristics of a new Freeze-dried kit, [6-hydrazinopyridine-3-carboxylic acid (HYNIC)]-GABA-Bombesin [7-14] NH2 designed for the labeling with 99mTc using tricine and EDDA as coligand. Methods: Synthesis was performed on a solid phase using a standard Fmoc strategy and HYNIC precursor coupled at the N-terminus. Purified peptide conjugate was labeled with 99mTc at 100°C for 10 min. Radiochemical analysis involved ITLC and high-performance liquid chromatography methods. Peptide conjugate stability and affinity to human serum was challenged for 24 hours. The internalization rate was studied in GRP receptor expressing PC-3 cells. Biodistribution of radiopeptide was studied in rats. Results: Radiolabeling was performed at high specific activities, and radiochemical purity was >98%. The stability of radiolabeled peptide in human serum was excellent. In vitro studies showed >14% of activity was specific internalized into PC-3 cells up to 4 h. After injection into rat biodistribution data showed a rapid blood clearance, with renal excretion and specific binding towards GRP receptor-positive tissues such as pancreas (1.15±0.19% ID/g after 4 h). Conclusion: [99mTc-HYNIC]-GABA-Bombesin [7-14] NH2 showed favorable radiochemical and biological characteristics which make our new designed labeled peptide conjugate as a very suitable agent for diagnostic purposes in malignant tumors.
کلیدواژه‌های انگلیسی مقاله Iran, meta-analysis, trend of infertilityGated myocardial perfusion SPECT, Quantification, segmental scoring, Meckel’s scan, Meckel’s diverticulum, Gastrointestinal bleeding, Technetium Pertechnetate scintigraphy, Thiosemicarbazone, 191Os, Radiolabeling, Biodistribution, RBBB, Myocardial perfusion, Gated SPECT, Coronary artery disease, Effective dose, Nuclear medicine procedures, Collective effective dose, Tc99m MIBI parathyroid scintigraphy, Myocardium perfusion gated SPECT, Parathyroid adenoma, Skeletal scintigraphy, Fluid restriction, Tc-99m MDP, Image quality, Bone to soft tissue ratio, Radiology, Nuclear medicine, Medical imaging, Bibliometric study, Iran, Hyperthyroidism, Radioactive iodine, Sexual hormones, Semen analysis, Attenuation correction, Artifact, Color intensity, SPECT, Thyroid, I-131, Absorbed ratio, Monte-Carlo, Absorbed dose, MCNP-4A, Carcinoid Tumor, Liver metastases, ¹³¹I-MIBG scintigraphy, Quality Control, PET, 82mRb, 82Kr, Myocardial perfusion, Drug delivery systems, Gamma-scintigraphy, Neutron activation, Pharmacoscintigraphy, Post stroke dementia, Cerebral perfusion SPECT, ECD, 99mTc-DTPA, Renography, Deconvolution, Renal transit time parameters, Nuclear medicine, PET tracer, Gallium, Copper, Tc-94m, Minimally invasive radio-guided surgery, Hyperparathyroidism, Tc-99m sestamibi, Parathyroid adenoma, Parathyroid hyperplasia, Bombesin, 99mTc, Tumor, HYNIC, Radioiodine therapy, TLD, Thyroid cancer, Absorbed dose, Myocardial perfusion scan, Ischemic heart disease, Risk factors, Demographic data, Military hospital, Evidence based medicine, Nuclear medicine, Evidence based medicine, Nuclear medicine, Critical appraisal, Sensitivity, Specificity, Cavernous hemangioma, Radionuclide imaging, 99mTc-RBC, Attenuation correction, Myocardial perfusion, SPECT, Radio-iodine therapy, Liothyronine, Thermoluminescent dosimeter, Accumulated dose, Exposure rate, 106Ru eye plaque, Dose calculation, Mathematical human eye model, MCNP4C code, Sentinel lymph node, Tc-99m antimony sulfide colloid, Lymphoscintigraphy, Skin marking, Osteoporosis, Type 2 diabetes, Postmenopausal, BMD, Streptokinase, Gallium-67, Thrombosis, SPECT, Radiopharmaceuticals, Breast uptake, Myocardial perfusion scan, Tc-99m sestamibi, Tc-99m pertechnetate scan, Meckel’s diverticulum, Gastrointestinal bleeding, PET, Sensitivity, SPECT, Spatial resolution, Temporal resolution, Dynamic imaging, Attenuation, Collimator, Random coincidence, Animal imaging, Dual isotope imaging, Time-of-Flight, Artifact, Bone scan, Catheter, PSA, Prostate, RIA, Standard matrix, Human serum, Myocardial perfusion scan, Leptin, BMI, [14C]Clozapine, Radiochemical purity, High performance liquid chromatography, Liquid scintillation spectrometer, Renography, Diuretic, 99mTc-DMSA, Differential renal function, Complications, Dipyridamole, Blood pressure changes, 99mTc-MIBI, Brain SPECT, Brain tumor recurrence, Radiation necrosis, Neuroendocrine tumors, Somatostatin receptors, Targeted therapy, Sm-153, EDTMP, Radiopharmaceutical, Therapy, Biological evaluation, Ursodeoxycholic acid, Hepatobiliary scintigraphy, 99mTc-BRIDA, Infantile jaundice, Neonatal hyperbilirubinemia, Positron emission tomography (PET), Scanner performance, Gate, Gallium-67 scintigraphy, Infection animal models, SPECT, Staphylococcus aureus, Escherichia coli, Candidae albicans, PET/CT, Dual energy CT, Attenuation correction, Von Hippel Lindau syndrome, Medullary carcinoma, PET/CT, Bone mlnearl density, Gruen zones, Orthoped software, Dual energy x-ray absorptiometry, Total hip arthroplasty, Clinical, PET/CT, fdg, Anatometabolic imaging, Oncology, Brain imaging, Cardiology, 2D vs. 3D, BGO, LSO, LYSO, GSO, Metastases, Medullary thyroid carcinoma, 99mTc-DMSA (V), Depression, Myocardial perfusion, Scintigraphy, SPECT, Liver hemangioma, Planar scan, Space occupying lesion, 99mTc-RBC, Attenuation correction, Myocardial perfusion, SPET, Emission scanning, Transmission scanning, Hybridoma, Radioimmunoassay technique, Protein G, Tyr3-Octreotide, Quality Control, Radiochemical purity, 99mTc ، Tyr3-Octreotate, PET imaging, Radiochemical, Fluorine-18, Myocardium, Scopus, Citation tracking, Google scholar, SCImago journal rank indicator, Iranian Journal of Nuclear Medicine, Indium-111, maltolate, Cell labeling, Biodistribution, Osteoporosis, discordance, densitometry, Menopause, Kidney transplantation, Myocardial perfusion, Myocardial function, Gated-SPECT, Tc-99m Phytate, Lymphoscintigraphy, Breast cancer, Sentinel node, Detection rate, FDG uptake in skeletal muscle, FDG PET/CT, FDG uptake phase, Exercise induced FDG uptake, Herniation, Urinary baladder, Bone scan, MDP, Diaphragmatic hernia, Intrathoracic kidney, Bowel in thorax, DTPA renogram, Tc-99m EC, Transplanted kidney, Vesicoureteral reflux, Renal scintigraphy, Salivary gland, Radioactive iodine, Xerostomia, Impact factor, Google scholar, h5-index, h5-median, SCImago journal rank indicator, Radiopharmaceutical, Trodat-1, Kit, Dopamine transporters, Thyroid cancer, complaints, quality of life, clinical symptoms, complication, Asymptomatic ischemia, Diabetes mellitus, Myocardial perfusion scintigraphy, Gated SPECT, AMD3100, Targeted radiotherapy, Radiolabeling, Biodistribution, Sm-153, 188Re-HEDP, Bone pain, Palliation therapy, Metastasis, Myocardial perfusion SPECT, Perfusion quantification, Polar map, Scoring system, MDP bone scan, FDG PET, Osteomyelitis, Necrotizing fasciitis, Acute myeloid leukemia, Avascular osteonecrosis, Multifocal, Alcohol, Bone scan, Polyarthritis, 99mTc-MIBI scientimammography, Breast cancer, Medical image analysis, Surface fitting, Contour detection, Curvature, Urinary tract infection, Vesico-ureteral reflux, Radioisotopic scan, Tc-99m DMSA, Renal scar, Acute myocardial infarction, Coronary Angiography, Coronary artery disease, SPECT, Head and neck irradiation, Thyroid cancer, Thyroid ultrasonography, Thyroid scan, Monoclonal antibody (MAB), Diethylentriaminepantaaceticacide (DTPA), Indium-111 (¹¹¹In), Fast performance liquid chromatography (FPLC), Chitosan, Holmium-166, Hepatic cancer, Radiosynovectomy, Biodistribution, Bombesin, HYNIC, Freeze-dried Kit, GRP receptors, Tumor,

نویسندگان مقاله 31160---31161---31162---31163---31164---31165---

نشانی اینترنتی http://irjnm.tums.ac.ir/pdf_559_6c55dd5674153369731dd3c790df1ac7.html
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده eng
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Articles
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات