1383/5/27، جلد ۱۳، شماره ۱، صفحات ۷-۱۶

عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله

عنوان انگلیسی 99mTc Direct radiolabeling of PR81, a new anti-MUC1 monoclonal antibody for radioimmunoscintigraphy
چکیده انگلیسی مقاله   Introduction: Monoclonal antibodies labeled mainly with 99mTc are being widely used as imaging agents in nuclear medicine. Recently PR81 was introduced as a new murine anti-MUC1 MAb against human breast carcinoma. This antibody reacts with the tandem repeat of 20-mer peptide of protein backbone, and is of IgG1 class, with an affinity of 2.19×10-8 M-1. Due to high specific reactivity, this antibody was shown to have high diagnostic potential in nuclear medicine. As the first step towards the use of an antibody for imaging purposes it has to be labeled with a radioisotope. In this study we have investigated a method for optimum labeling of this MAb with 99mTc. Materials and Methods: The Ab reduction was performed with 2-mercaptoethanol (2-ME) at a molar ratio of 2000:1 (2-ME:MAb) and reduced Ab was labeled with 99mTc via stannous tartrate as a transchelator. The labeling efficiency was determined by ITLC. The integrity of reduced MAb was checked by means of SDS-PAGE and gel filtration chromatography (FPLC) on Superose 12 HR 10/30 (purity>99%). The amount of radiocolloids were measured by cellulose nitrate electrophoresis. In vitro stability of labeled product was checked in time intervals over 24 hrs by ITLC. Radioimmunoassay was used to test immunoreactivity of the labeled MAb. Biodistribution of the radiolabeled MAb was studied in normal mice at 4 and 24 hrs post-injection. Results: The labeling efficiency was %96±2.8 with high invitro stability (%82±2.7 in 24 hrs) and less than %2 radiocolloids were found. There was no Ab fragmentation due to reduction procedure. Both labeled and unlabeled MAbs were able to compete for binding to the MUC1. Biodistribution studies in normal mice showed that there was no significant accumulation in any organ. Conclusion: Because of no significant loss of immunoreactivity of MAb due to labeling procedure, high in vitro stability and no accumulation in vital organs, 99m-Tc-PR81 may be a promising candidate for radioimmunoscintigraphy studies of breast cancer.
کلیدواژه‌های انگلیسی مقاله Iran, meta-analysis, trend of infertilityGated myocardial perfusion SPECT, Quantification, segmental scoring, Meckel’s scan, Meckel’s diverticulum, Gastrointestinal bleeding, Technetium Pertechnetate scintigraphy, Thiosemicarbazone, 191Os, Radiolabeling, Biodistribution, RBBB, Myocardial perfusion, Gated SPECT, Coronary artery disease, Effective dose, Nuclear medicine procedures, Collective effective dose, Tc99m MIBI parathyroid scintigraphy, Myocardium perfusion gated SPECT, Parathyroid adenoma, Skeletal scintigraphy, Fluid restriction, Tc-99m MDP, Image quality, Bone to soft tissue ratio, Radiology, Nuclear medicine, Medical imaging, Bibliometric study, Iran, Hyperthyroidism, Radioactive iodine, Sexual hormones, Semen analysis, Attenuation correction, Artifact, Color intensity, SPECT, Thyroid, I-131, Absorbed ratio, Monte-Carlo, Absorbed dose, MCNP-4A, Carcinoid Tumor, Liver metastases, ¹³¹I-MIBG scintigraphy, Quality Control, PET, 82mRb, 82Kr, Myocardial perfusion, Drug delivery systems, Gamma-scintigraphy, Neutron activation, Pharmacoscintigraphy, Post stroke dementia, Cerebral perfusion SPECT, ECD, 99mTc-DTPA, Renography, Deconvolution, Renal transit time parameters, Nuclear medicine, PET tracer, Gallium, Copper, Tc-94m, Minimally invasive radio-guided surgery, Hyperparathyroidism, Tc-99m sestamibi, Parathyroid adenoma, Parathyroid hyperplasia, Bombesin, 99mTc, Tumor, HYNIC, Radioiodine therapy, TLD, Thyroid cancer, Absorbed dose, Myocardial perfusion scan, Ischemic heart disease, Risk factors, Demographic data, Military hospital, Evidence based medicine, Nuclear medicine, Evidence based medicine, Nuclear medicine, Critical appraisal, Sensitivity, Specificity, Cavernous hemangioma, Radionuclide imaging, 99mTc-RBC, Attenuation correction, Myocardial perfusion, SPECT, Radio-iodine therapy, Liothyronine, Thermoluminescent dosimeter, Accumulated dose, Exposure rate, 106Ru eye plaque, Dose calculation, Mathematical human eye model, MCNP4C code, Sentinel lymph node, Tc-99m antimony sulfide colloid, Lymphoscintigraphy, Skin marking, Osteoporosis, Type 2 diabetes, Postmenopausal, BMD, Streptokinase, Gallium-67, Thrombosis, SPECT, Radiopharmaceuticals, Breast uptake, Myocardial perfusion scan, Tc-99m sestamibi, Tc-99m pertechnetate scan, Meckel’s diverticulum, Gastrointestinal bleeding, PET, Sensitivity, SPECT, Spatial resolution, Temporal resolution, Dynamic imaging, Attenuation, Collimator, Random coincidence, Animal imaging, Dual isotope imaging, Time-of-Flight, Artifact, Bone scan, Catheter, PSA, Prostate, RIA, Standard matrix, Human serum, Myocardial perfusion scan, Leptin, BMI, [14C]Clozapine, Radiochemical purity, High performance liquid chromatography, Liquid scintillation spectrometer, Renography, Diuretic, 99mTc-DMSA, Differential renal function, Complications, Dipyridamole, Blood pressure changes, 99mTc-MIBI, Brain SPECT, Brain tumor recurrence, Radiation necrosis, Neuroendocrine tumors, Somatostatin receptors, Targeted therapy, Sm-153, EDTMP, Radiopharmaceutical, Therapy, Biological evaluation, Ursodeoxycholic acid, Hepatobiliary scintigraphy, 99mTc-BRIDA, Infantile jaundice, Neonatal hyperbilirubinemia, Positron emission tomography (PET), Scanner performance, Gate, Gallium-67 scintigraphy, Infection animal models, SPECT, Staphylococcus aureus, Escherichia coli, Candidae albicans, PET/CT, Dual energy CT, Attenuation correction, Von Hippel Lindau syndrome, Medullary carcinoma, PET/CT, Bone mlnearl density, Gruen zones, Orthoped software, Dual energy x-ray absorptiometry, Total hip arthroplasty, Clinical, PET/CT, fdg, Anatometabolic imaging, Oncology, Brain imaging, Cardiology, 2D vs. 3D, BGO, LSO, LYSO, GSO, Metastases, Medullary thyroid carcinoma, 99mTc-DMSA (V), Depression, Myocardial perfusion, Scintigraphy, SPECT, Liver hemangioma, Planar scan, Space occupying lesion, 99mTc-RBC, Attenuation correction, Myocardial perfusion, SPET, Emission scanning, Transmission scanning, Hybridoma, Radioimmunoassay technique, Protein G, Tyr3-Octreotide, Quality Control, Radiochemical purity, 99mTc ، Tyr3-Octreotate, PET imaging, Radiochemical, Fluorine-18, Myocardium, Scopus, Citation tracking, Google scholar, SCImago journal rank indicator, Iranian Journal of Nuclear Medicine, Indium-111, maltolate, Cell labeling, Biodistribution, Osteoporosis, discordance, densitometry, Menopause, Kidney transplantation, Myocardial perfusion, Myocardial function, Gated-SPECT, Tc-99m Phytate, Lymphoscintigraphy, Breast cancer, Sentinel node, Detection rate, FDG uptake in skeletal muscle, FDG PET/CT, FDG uptake phase, Exercise induced FDG uptake, Herniation, Urinary baladder, Bone scan, MDP, Diaphragmatic hernia, Intrathoracic kidney, Bowel in thorax, DTPA renogram, Tc-99m EC, Transplanted kidney, Vesicoureteral reflux, Renal scintigraphy, Salivary gland, Radioactive iodine, Xerostomia, Impact factor, Google scholar, h5-index, h5-median, SCImago journal rank indicator, Radiopharmaceutical, Trodat-1, Kit, Dopamine transporters, Thyroid cancer, complaints, quality of life, clinical symptoms, complication, Asymptomatic ischemia, Diabetes mellitus, Myocardial perfusion scintigraphy, Gated SPECT, AMD3100, Targeted radiotherapy, Radiolabeling, Biodistribution, Sm-153, 188Re-HEDP, Bone pain, Palliation therapy, Metastasis, Myocardial perfusion SPECT, Perfusion quantification, Polar map, Scoring system, MDP bone scan, FDG PET, Osteomyelitis, Necrotizing fasciitis, Acute myeloid leukemia, Avascular osteonecrosis, Multifocal, Alcohol, Bone scan, Polyarthritis, 99mTc-MIBI scientimammography, Breast cancer, Medical image analysis, Surface fitting, Contour detection, Curvature, Urinary tract infection, Vesico-ureteral reflux, Radioisotopic scan, Tc-99m DMSA, Renal scar, Acute myocardial infarction, Coronary Angiography, Coronary artery disease, SPECT, Head and neck irradiation, Thyroid cancer, Thyroid ultrasonography, Thyroid scan, Monoclonal antibody (MAB), Diethylentriaminepantaaceticacide (DTPA), Indium-111 (¹¹¹In), Fast performance liquid chromatography (FPLC), Chitosan, Holmium-166, Hepatic cancer, Radiosynovectomy, Biodistribution, Bombesin, HYNIC, Freeze-dried Kit, GRP receptors, Tumor, 99mTc-UBI 29–41, Ubiquicidin, Diabetic foot, Infection, Radiolabeled peptide, Radiolabeling, Quality Control, [64Cu]ATSM, Rabbits, Biodistribution, Coincidence imaging, Evidence based medicine, Journal club, Nuclear medicine, Critical appraisal, 99mTc- ciprofloxacin, Infection imaging, Kit preparation, Cuboid fracture, Bone Scintigraphy, Limping, Toddler fracture, Bone scan, Visualization of colon, Soft tissue uptake, Tc-99m MDP, Radioiodine, Hyperthyroidism, Toxic goiter, Toxic nodule, I-131, 67Gallium, SPECT, Planar imaging, Lymphoma, Time of imaging, 8-Hydroxy-2-methylquinoline, In-111, Biodistribution, SPECT, Hyperthyroidism, Hypothyroidism, Differentiated thyroid carcinoma, Echocardiography, Tissue doppler imaging, Calcitonin, Radiolabeling, Biodistribution, Ga-67, Coronary artery disease, Myocardial perfusion imaging, Coronary artery calcification score, SPM, SPECT, Attenuation correction, Activation foci, Sm-153, TTHMP, Radiopharmaceutical, Therapy, Biodistribution, Imaging, MRI, Bone SPECT, Meniscal tear, Monoclonal antibody, MUC1, PR81, Technetium-99m, Radiolabeling,

نویسندگان مقاله 31265---31266---31267---31268---31269---31270---31271---31272---

نشانی اینترنتی http://irjnm.tums.ac.ir/pdf_452_0e0b69f0d8d83c3b4eb13b37e179b43c.html
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده eng
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Articles
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات