Volume 56, Issue 5 (6 1998)
Tehran Univ Med J 1998, 56(5): 9-18 |
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Arvan R, Elahy E. Synthesis of human placental CDNA and demonstration of the expression of M-CSF in that tissue. Tehran Univ Med J 1998; 56 (5) :9-18
URL: http://tumj.tums.ac.ir/article-1-1515-en.html
URL: http://tumj.tums.ac.ir/article-1-1515-en.html
Abstract: (7238 Views)
Macrophage colony stimulating factor (M-CSF) has previously been shown to affect the differentiation of cells of the mono-nuclear phagocytic line. More recent studies indicate that M-CSF may have a role in pregnancy. In the present study, the expression of M-CSF in the human placenta was demonstrated. Placental mRNA was isolated and used as template for synthesis of complementary DNA (cDNA). The presence of M-CSF related sequences in the cDNA was shown by PCR and RT-PCR reactions in which M-CSF specific primers were used. In addition, it was shown that a 2.4 kb cDNA after electrophoresis and transfer to a nylon filter, hybridized with a digoxygenin labeled M-CSF specific probe.
Keywords: Macrophage colony stimulating factor (M-CSF), Messenger RNA (mRNA), Complementary DNA (CDNA), Polymerase chain reaction (PCR), Reverse transcriptase-polymerase chian reaction (RT-PCR), Digoxygenin
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