Volume 68, Issue 8 (November 2010)
Tehran Univ Med J 2010, 68(8): 444-450 |
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Amirzargar A A, Mahmoodi M, Nahvi H, Kasaian A, Safari Z, Mahmoudi M, et al . Association study between HLA-DRB, HLA-DQA1, HLA-DQB1 and breast cancer in Iranian women. Tehran Univ Med J 2010; 68 (8) :444-450
URL: http://tumj.tums.ac.ir/article-1-5649-en.html
URL: http://tumj.tums.ac.ir/article-1-5649-en.html
Ali Akbar Amirzargar1 
, Majid Mahmoodi * 2, Hedayat Nahvi3 , Amir Kasaian3 , Zahra Safari3 , Mahdi Mahmoudi1 , Yadolla Shekiba1 , Kouros Divsalar4 , Abbas Jafari3 , Bita Ansarpour1 , Batool Moradi1 , Mohammad-Ali Mohagheghi3
, Majid Mahmoodi * 2, Hedayat Nahvi3 , Amir Kasaian3 , Zahra Safari3 , Mahdi Mahmoudi1 , Yadolla Shekiba1 , Kouros Divsalar4 , Abbas Jafari3 , Bita Ansarpour1 , Batool Moradi1 , Mohammad-Ali Mohagheghi3
1- Department of Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
2- Cancer Research Center, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran. ,dmahmoodi@razi.tums.ac.ir
3- Cancer Research Center, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran.
4- Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran.
2- Cancer Research Center, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran. ,
3- Cancer Research Center, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran.
4- Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Abstract: (5350 Views)
Background: Based on the reports, high frequency of special alleles of HLA class II genes might be associated with susceptibility to or protective from a particular cancer. These alleles might vary depending on the geographical region. Here we investigate the association between alleles of HLA class II genes and breast cancer in Iranian women.
Methods: 100 patients with pathologically proved breast cancer who referred to Cancer Institute, Tehran University of Medical Sciences in Tehran, Iran, were divided to two groups based on ages (40 years old and less/ or more than 40 years old) and were randomly selected and compared with a group of 80 healthy blood donor subjects. HLA class II alleles were determined by amplification of DNA with polymerase chain reaction (PCR) method followed by HLA-typing using sequence-specific primer (SSP) for each allele.
Results: The most frequent alleles in the DR and DQ regions in group 1 (40 years old and less) in comparison with control group were HLA-DQA1*0301 (p=0.002) and HLA-DQB1*0302 (p>0.05). In contrast HLA-DQA1*0505 (p=0.004) had significantly lower frequency in this group compared with control group. Patients of group two (more than 40 years old) had a higher frequencies of HLA-DQA1*0301 (p=0.001) and HLA-DRB1*1303 (p=0.02) and a lower frequency of HLA-DQA1*0101 (p=0.002) compared to healthy control.
Conclusion: These findings provide information of a positive and negative association between certain alleles of HLA class II and breast cancer in our population and also might support that the pattern of inheritance in the early and late onset of breast cancer differ substantially.
Methods: 100 patients with pathologically proved breast cancer who referred to Cancer Institute, Tehran University of Medical Sciences in Tehran, Iran, were divided to two groups based on ages (40 years old and less/ or more than 40 years old) and were randomly selected and compared with a group of 80 healthy blood donor subjects. HLA class II alleles were determined by amplification of DNA with polymerase chain reaction (PCR) method followed by HLA-typing using sequence-specific primer (SSP) for each allele.
Results: The most frequent alleles in the DR and DQ regions in group 1 (40 years old and less) in comparison with control group were HLA-DQA1*0301 (p=0.002) and HLA-DQB1*0302 (p>0.05). In contrast HLA-DQA1*0505 (p=0.004) had significantly lower frequency in this group compared with control group. Patients of group two (more than 40 years old) had a higher frequencies of HLA-DQA1*0301 (p=0.001) and HLA-DRB1*1303 (p=0.02) and a lower frequency of HLA-DQA1*0101 (p=0.002) compared to healthy control.
Conclusion: These findings provide information of a positive and negative association between certain alleles of HLA class II and breast cancer in our population and also might support that the pattern of inheritance in the early and late onset of breast cancer differ substantially.
Type of Study: Original Article |
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