Volume 73, Issue 2 (May 2015)
Tehran Univ Med J 2015, 73(2): 127-131 |
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Shojaa M, Aghaie M, Amoli M, Khashayar P, Javid N, Shakeri F, et al . Association between CTLA-4 gene polymorphism and the risk of systemic lupus erythematosus: brief report. Tehran Univ Med J 2015; 73 (2) :127-131
URL: http://tumj.tums.ac.ir/article-1-6609-en.html
URL: http://tumj.tums.ac.ir/article-1-6609-en.html
Mahdieh Shojaa1 
, Mehrdad Aghaie * 2, Mahsa Amoli3 , Patricia Khashayar4 , Naemeh Javid5 , Fatemeh Shakeri5 , Mostafa Qorbani6 , Ramin Mohebbi7
, Mehrdad Aghaie * 2, Mahsa Amoli3 , Patricia Khashayar4 , Naemeh Javid5 , Fatemeh Shakeri5 , Mostafa Qorbani6 , Ramin Mohebbi7
1- Medicine, Golestan University of Medical Sciences, Gorgan, Osteoporosis Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
2- Bone Joint and Connective Tissue Disease Research Center (BJCRC), Department of Rheumatology, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran. ,aghaie1390@gmail.com
3- Endocrinology and Metabolism Research Center (EMRC), Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
4- Nano biotechnology, Osteoporosis Research Center, Tehran University of Medical Sciences, Tehran, Iran.
5- Department of Internal Medicine, Golestan University of Medical Sciences, Golestan, Iran.
6- Department of Public Health, Alborz University of Medical Sciences, Karaj, Iran, Non-Communicable Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
7- General Practitioner, Shahed University of Medical Sciences, Tehran, Iran.
2- Bone Joint and Connective Tissue Disease Research Center (BJCRC), Department of Rheumatology, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran. ,
3- Endocrinology and Metabolism Research Center (EMRC), Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
4- Nano biotechnology, Osteoporosis Research Center, Tehran University of Medical Sciences, Tehran, Iran.
5- Department of Internal Medicine, Golestan University of Medical Sciences, Golestan, Iran.
6- Department of Public Health, Alborz University of Medical Sciences, Karaj, Iran, Non-Communicable Diseases Research Center, Endocrinology and Metabolism Population Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
7- General Practitioner, Shahed University of Medical Sciences, Tehran, Iran.
Abstract: (5750 Views)
Background: Cytotoxic lymphocyte antigen-4 (CTLA-4) plays an important role in regulating T cell activation. CTLA-4 gene polymorphisms are related with genetic susceptibility to various autoimmune diseases, including systemic lupus erythematosus (SLE). We analyzed the role of CTLA-4 polymorphisms at positions -318CT in patients who suffer from SLE.
Methods: This study was performed on 180 SLE patients referred to 5th Azar University Hospital in Gorgan, Iran. Three hundred and four ethnically-and age-matched healthy controls with no history of autoimmune diseases entered the study between 5th May 2008 and 23rd October 2009. DNA was extracted from blood samples according to the standard procedure. Polymerase chain reaction- restriction fragments length polymorphism (PCR-RFLP) was used to analyze the genotype and allele frequencies of this polymorphism. PCR was carried out using the following primers: forward 5′-AAATGAATTGGACTGGATGGT-3′ and reverse 5′-TTACGAGAAAGGAAGCCGT G-3′. The frequency of alleles and genotypes were assessed using direct counting. Chi-square test and Fisher’s exact test were used to compare the association between the alleles and genotype frequencies and SLE. P<0.05 were considered statistically significant.
Results: The CC genotype was observed in 94.5% of the SLE patients and 82.4% of the controls the difference was statistically significant (P=0.0001, OR=3.51, CI95%=1.77-7.53). The CT genotype, on the other hand, was more frequently observed in the control group (17.1% vs. 5.5%, P=0.0001, OR=0.28). T allele was significantly more common in the controls compared to SLE patients (P=0.0001, OR=0.26, CI95%=0.13-0.53).
Conclusion: Our results suggest that the -318C/T polymorphism of CTLA-4 gene might play a significant role in the genetic susceptibility to SLE. Therefore, further studies on populations, especially from other Middle East countries, are needed to confirm our results.
Type of Study: Brief Report |
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