Showing 3 results for Bidmeshkipour
Ali Bidmeshkipour, Abaslt Hosseinzadeh Colagar, Maryam Gholinezhad Chari, Pourya Biparva,
Volume 67, Issue 12 (6 2010)
Abstract
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Background: Defective sperm function is now recognized as
one of the most important causes of male infertility. Seminal plasma possesses
a rich source of different enzymatic and
non-enzymatic antioxidants such as vitamin C (ascorbic
acid) that protect spermatozoa against oxidative stress as one of the mediators
of infertility causing sperm dysfunction and low sperm quality. The
aim of this study was investigation of seminal total antioxidant capacity and determination
of vitamin C effects on sperm
motility.
Methods: We designed a case-control study with a total
subject of 62 males. Sperm
parameters were analyzed according to World
Health Organization
guidelines (WHO, 1999).
Total antioxidant capacity and vitamin C
level of seminal plasma were measured in the 32
normozoospermic as the control group and 32
asthenospermic men as the case group using FRAP
(Ferric Reducing of Antioxidants
Powers)
and RP-HPLC (Reverse Phase
High Performance
Liquid Chromatography)
methods, respectively.
Results: Our results indicated that total antioxidant
capacity levels in the seminal plasma of asthenospermic men were significantly
lower than healthy men (p=0.002).
In addition, we found a positive correlation
between reduced total antioxidant capacity levels and low sperm motility. Vitamin
C levels of seminal plasma in asthenospermic men
were statistically lower than control men (p=0.01).
Conclusions: It is suggested that asthenospermia could be
related to an antioxidant deficiency or it&aposs reduction.
Hesari M, Mansouri K, Mostafaie A, Bidmeshkipour A,
Volume 68, Issue 3 (5 2010)
Abstract
Background: Proteolytic enzymes, especially collagenases, are used for digestion of
extracellular matrix, cell isolation and primary culture. Because of the problems in purification and low amount of collagenases in bacterial or animal sources, it is important to find new sources of the enzymes. So, in the present study actinidin, a plentiful protein in kiwifruit was purified and a mixture of actinidin and trypsin was applied to isolate rat aortic endothelial cells.
Methods: Aortic endothelial cells were isolated using digestion solution containing different concentrations of actinidin (from 2 to 16 mg/ml) and trypsin (0.3, 0.6, 1.2 and 2.4 mg/ml) in different times (from 15 to 90 minute). Isolated cells were cultured in
DMEM culture medium. Isolated cells were identified by morphological characteristics and immunocytochemical staining viability of separated cells was estimated by trypan blue exclusion test.
Results: Actinidin in concentration of 10 mg/ml with trypsin in concentration of 1.2 mg/ml for one hour could isolate rat aortic endothelial cells. In this condition the viability of cells was estimated 90%. Morphological and immunocytochemical charac- teristics confirmed the isolated cells as endothelial cells.
Conclusion: The results showed that the mentioned mixture of actinidin and trypsin has not considerable toxic effects on separated cells and is a novel and suitable option for isolation of rat aortic endothelial cells
Reza Yarani , Kamran Mansouri , Ali Bidmeshkipour , Maryam Mehrabi , Ali Ebrahimi , Kaikaoos Gholami , Kheirollah Yari , Ali Mostafaie ,
Volume 71, Issue 3 (June 2013)
Abstract
Background: Primary culture takes place following the cell isolation from tissues. Isolation and culture of melanocytes based on their roll in the protection of body against hazardous sun rays, production of skin, cornea and hair color is really important. This study was done to set isolation, culture and proliferation of melanocytes from children foreskin and adult eyelashes, and also comparison of two types of melanocyte culture medium.
Methods: Human foreskin and eyelash samples were used for melanocyte isolation and culture. After isolation of epidermis from dermis, epidermis cell suspensions were prepared by enzymatic digestion. The isolated cells were cultured in two melanocyte selective culture media. Immunocytochemistary and reverse transcription-polymerase chain reaction (RT-PCR) assays were used for confirmation of isolated and cultured melanocytes.
Results: Our results indicated that isolated melanocyte cultured in the selective medium without phorbol esters is better than the melanocytes cultured in selective medium cont-aining phorbol esters not only morphologically but also physiologically and from the aspect of cell adhesion. In addition, the results showed that isolated melanocyte from adult eyelashes are more dendritic than melanocytes isolated from children foreskin. Conversely, our results indicated that the number of cell passages in melanocyte isolat-ed from foreskin is more than melanocytes isolated from adult eyelashes.
Conclusion: Melanocytes cultured in selective medium containing convenient growth factors in absence of phorbol esters show more native physiological and adhesive properties. In addition, melanocyte isolated from younger tissues such as foreskin have better proliferative and sub-culturing properties so we suggest isolation and culture of younger tissues.
