Background and Aim: Helicobacter pylori is the etiologic agent of chronic –active gastritis, gastroduodenal ulcers in humans, and a co-factor in the occurrence of gastric cancer and mucosa-associated lymphoid tumors, Adhesion of H.pylori to the gastric mucosa is a critical and also initial step in the pathogenesis of the disease. Bacterial adhesion inhibitory agents provide a novel pharmacologic approach to the management of infectious diseases.
Materials and Methods: 22 H. pylori strains, isolated from the antral biopsies of 49 patients with dyspepsia, gastritis, gastric ulcer, duodenal ulcer,…were assayed by ELISA (UPR)to investigate the diversity of attachment to 7 mamalian cell lines.
Results: The concentration of H.pylori and cell suspention ,the condition and temperature, can alter the attachment rate.Best bacterial concentration was equal to 1 Mc farland,and for cell suspension was 5*10 cells/ml.90 minutes in 37C incubation period result in maximum attachment. H.pylori can attach to all 7 cell lines, there are no significant differences between 22 H.pylori strains in attachment to cells. The attachment pattern of H.pylori to the cells showed significant reduction respectly from HepII, HeLa, SW742, AGS,HT29/219, HT29 to Caco-2.Maximum attachment were seen to HepII, HeLa and SW742 cells, and among these HepII was the best cells for this purpose.
Conclusion: Our studies suggest that Hep II, HeLa and SW742 cells could serve as a suitable in-vitro model for the study of H.pylori adhesions, attachment, inhibition of attachment and detachment assays and among these Hep II cell is prefer recommended.
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Methods: Sixty patients with various degrees of
fatty infiltration on liver biopsy and twenty normal subjects without any sign
of hepatic fat infiltration in ultrasonography examined using standard colour
and spectral doppler sonography. The waveforms of Hepatic Vein were classified
into three groups: regular triphasic waveform, biphasic waveform without a
reverse flow, and monophasic or flat waveform. The hepatic artery resistance
index was calculated as the mean of three different measurements.
Results: The mean BMI in Nonalcoholic fatty liver disease group and normal subjects was 26.9(SD=3.3) and 22.4(SD=1.7) Kg/m2, respectively with a
range of 22
up to 44 Kg/m2. Abnormal Hepatic Vein
waveforms (biphasic and monophasic) were found more frequently in doppler
sonography (p<0.001)
in patients with Nonalcoholic fatty liver disease (12 biphasic and 17 monophasic) compared to normal subjects. Hepatic artery resistance
index was significantly lower in Nonalcoholic fatty liver disease patients [0.7(SD=0.1)] compared to normal
ones [0.8(SD=0.0)]
(p<0.001).
Conclusions: The incidence of abnormal hepatic vein waveforms is significantly higher in
patients with fatty infiltration compared to those who had no abnormality in
liver ultrasonography and these patients had a significant lower hepatic artery
resistance index. supportFields]> ADDIN EN.CITE ADDIN EN.CITE.DATA
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