K Fooladsaz , M Rasaei , M Ansari ,
Volume 57, Issue 4 (9 1999)
Abstract
Nowadays immunochemical techniques have played a very important and valuable role in quantitative and qualitative assays of liquid compounds of the body. Producing antibody against immunogenes is the first step to make immunochemical kits. In this study production and purification of polyclonal antibody against melatonin has been considered. This hormone which has several important functions in physiological conditions such as migraine, cirrhosis, mammary gland cancer and other diseases, is the most important pineal gland secretion. This gland is a circumventricular organ of brain and according to histological and anatomical studies, it is a high secretory organ, that secretes active biological substances like melatonin, oxytocin, serotonin and ect. In this study, melatonin has been considered as hapten and has become an immunogen by being linked to the bovine serum Albumin. Then, by the immunization of three white New Zeland rabbits that had the booster injections in regular intervals, the antibody titer was detected to be 1/2000, by using checkboard curves, and with the use of melatonin linked to penicillinase as a labeled antigen, the titer was detected 1/200. Finally an antibody with high purification rate has been obtained, which can be used in immunochemical assays like RIA, ELISA, and EIA.
Fooladsaz K, Ansari M, Javad Rassaie M,
Volume 62, Issue 1 (12 2004)
Abstract
Background: Migraine is a chronic hereditary and relapsing headache. With regard to the prevalence of this ancient disease and its economic complications in country, in this study , nocturnal serum melatonin of migraine patients and control subjects have been evaluated and compared by ELISA kit.
Materials and Methods: Fifty migraine patients (mostly women) were compared to a control group (mostly men) matched according to age.
Results: Statistical analysis revealed a decrease in nocturnal serum melatonin levels for migraine patients (32.9 28.4) compared to the control one (75.6 56.8). With using of t-test by ELISA kit showed significant difference (p=0.0064).
Conclusion: With regard to this, the pineal gland has the main role in the synchronization of the organism with the environmental conditions and migrainous headaches.
Derkhshandeh J, Fooladsaz K, Heydarpour F,
Volume 67, Issue 8 (6 2009)
Abstract
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Background: Dietary antioxidant vitamins, in particular vitamin C,
can play a role in preventing the onset or progression of age-related visual
impairment. The aim of this study was to determine the plasma ascorbic acid
levels in normal individuals and in senile cataract patients.
Methods: The test group included fifty cataract patients, while the control group
members were selected among medical staff and patients' companions after age
matching with test group. The use questionnaire contained age, sex and location
of residency (Urban or Rural). Five milliliter of drawn blood centrifuged and
plasma ascorbic acid level was measured by spectrophotometry. Statistical
analysis of plasma ascorbic acid concentrations were done using paired t-test.
Results: The mean plasma ascorbic acid level intest group and incotral group was 0.96±0.08
and 1.12±0.15mg/dl respectively.
Plasma ascorbic acid level in test group was significantly lower than control
group (p<0.0001).
Aging was associated with a reduction in plasma ascorbic acid level in male
while it showed no significant alteration in females. Rural and the female
gender showed higher plasma ascorbic acid level.
Conclusions: This study revealed plasma ascorbic acid level in patients with senile cataract
was lower than normal individuals. Antioxidant vitamins, in particular vitamin C,
found in Mediterranean fruits and vegetables could be probably used in the
prevention of cataracts, which is a major health service burden in many
countries.
