Showing 20 results for GR
H Sadeghipoor , M Vosooghi , M Mirnoori Langroody ,
Volume 56, Issue 4 (1 1998)
Abstract
Due to the important role of the trace elements (i.e. zinc and copper) in the biochemical processes of body and the effects of exogenous and endogenous hormones in distribution, absorption and excretion of these elements, we decided to determine the rate of the zinc and copper content of serum in women taking (LD) OCPs. Our finding indicates that the rate of the zinc content decreased significantly (P<0.001), whereas the copper content of women serum during this period showed a significant increase (P<0.001). In regard to previous months, the process of increasing were lasting for six months. Therefore, we would like to suggest that the serum content of these two elements should be monitored during the period of medication with OCPs, to avoid the deleterious effect of flactuation rates of these elements in serum.
Firoozbakhsh Sh, Safavi E, Zahed Poor Anaraki Mr, Derakhshan Deilami Gr ,
Volume 60, Issue 3 (14 2002)
Abstract
Background: Transbronchial lung biopsy (TBLB) is an attractive alternative to open lung biopsy as an initial diagnostic procedure for patients with diffuse parenchymal disease or localized densities beyond direct endoscopic vision. TBLB can be carried out safely without fluoroscopy in patients with diffuse lung disease. Since in our bronchoscopic department fluoroscopy is not available, we planned to evaluate the blind (without fluoroscopic guide) TBLB being performed in our department to determine the success rate in obtaining lung tissue, the sensitivity of the procedure and the risk of complications.
Materials and Methods: Sixty-Four TBLB were done in our department during a 6 month period (March-September 1999).
Results: Lung tissue wasn’t detected in two (3.1 percent) samples. Pathological results were helpful in 46 (71.9 percent) cases. No evidence of hemoptysis was found after the procedure. Three (4.68 percent) cases of pneumothorax was detected. Only one of them required chest tube (1.51 percent).
Conclusion: We concluded that blind TBLB was successful in our department with rates of complications comparable to other approved centers.
Hadian Mr, Otadi K, Oliaei Gr, Talebian Moghaddam S ,
Volume 60, Issue 3 (14 2002)
Abstract
Introduction: It is suggested that quadriceps muscle has an important role in stability & mobility of Knee joint in athletics and normal individuals therefore, the purpose of this study was evaluation of the strength in Power Athletics (PA) and Normal Un-trained Individuals (NUI) groups.
Methods and Materials: 31 Females (20 NUI & 11 PA) participated in this study. For measuring the strength, each individual performed 5 continuous concentric-eccentric maximal contraction at angular velocities of 90°/s, 135°/s. Twenty five percent of each individual (Maximum Voluntary Isometric Contraction) MVIC was determined and used as Pre-load force.
Results: Averages concentric & eccentric torques were greater in PA group in comparison with NUI group. The significant difference (P<0.02) existed between PA and NUI groups. Average concentric torques of quadriceps muscle decreased (with increasing of speed from 90°/s to 135°/s) and average eccentric torques increased. Average eccentric torques were greater (P<0.01) in PA & NUI groups in comparison with average concentric torques.
Conclusion: PA group strength was greater in comparison with NUI group. This is possibly due to the type of muscle fibers in this group have (greater type II fibers). Accordingly, it is critical to consider the role of eccentric exercise in PA group for preventing sport injury.
Kalbasi G, Talebian Moghaddam S, Ebrahimi Takamjani S, Oliaei Gr, Maroofi N, Galaei S,
Volume 63, Issue 2 (12 2005)
Abstract
Background: One of the most important concerns in orthopedic medicine is the low back. Considering the importance of muscle function in preventing LBT by controlling too much load and stress applied on the spinal joints and ligaments.
Materials and Methods: The aim of this research was to determine the timing and level of activities of lumbopelvic muscles in response to postural perturbations caused by unexpected loading of the upper limbs in standing on three different supporting surfaces (neutral, positive slope, negative slope) in 20 healthy females 18 to 30 years old ( = 23.20 SD = 2.55 ). The electromyographic signals were recorded from the deltoid, gluteus maximus, internal oblique abdominis and lumbar paraspinal muscles of the dominant side of the body to evaluate the onset time, end time, level of muscle activity (RMS) and duration of different muscles in one task and one muscle in different tasks.
Results: The results showed that the agonists (posterior muscles) activated at first to compensate the flexor torque caused by loading and then the antagonists (anterior muscles) switched-on to compensate the reaction forces caused by agonist activities. With regards to continuous activity of internal oblique and its attachments via thoracalumbar fascia to the transverse processes of the lumbar vertebrae, it can be considered as one of the major stabilizer muscles of the trunk .
Conclusion: Finally the results indicated that supporting surface type didn’t have any effect on timing and scaling of muscle activities in different tasks suggesting that probably spinal and trunk priprioceptors are just responsible for triggering postural responses and they don’t have any role in determining timing and scaling.
J. Mehdizadeh, R. Safikhani, M. Motiee Langroudi,
Volume 64, Issue 5 (1 2006)
Abstract
Background: Prolonged endotracheal intubation is a growing method for supporting ventilation in patients who require intensive care. Despite considerable advancement in endotracheal intubation, this method still has some complications the most important is laryngo-tracheal injuries.
Methods: Over a 2-year period, this retrospective study was conducted on 57 patients with history of prolonged intubation who were referred to the ENT Department of Amir Alam Hospital. For each patient, a complete evaluation including history, physical examination, and direct laryngoscopy and bronchoscopy was done under general anesthesia.
Results: Fifty-seven patients (44 male mean age, 23.014.7 years) were studied. Mean intubation period was 15.88 days. The most common presenting symptom was dyspnea (62%). Head trauma was responsible for most cases of intubation (72.4%). The most common types of tracheal and laryngeal lesions were tracheal (56.9%) and subglottic (55.2%) stenosis, respectively. Mean length of tracheal stenosis was 0.810.83 cm. There was a statistically significant relationship between length of tracheal stenosis and intubation period (P=0.0001) but no relation was observed between tracheal stenosis and age, sex, and etiology of intubation (All P=NS). Among the glottic lesions, inter- arytenoids adhesion was the most common lesion (25.9%). No statistically significant relation was found between glottic and subglottic lesions and age, sex and intubation period (all P=NS). Length of stenosis and intubation period was significantly greater in tracheal/ subglottic lesions than those in glottic/ supraglottic lesions (all P=NS).
Conclusion: After prolonged endotracheal intubation, laryngo-tracheal lesions had no relation with patient’s age, sex, and cause of intubation.There was direct relation between length of tracheal stenosis and intubation period. Glottic lesions were more commonly observed in head trauma patients. Lesion length and intubation period were greater in tracheal and subglottic lesions.
Talebian S, Bahrami Sh, Olyaei Gr, Bagheri H, Hadian Mr,
Volume 64, Issue 7 (9 2006)
Abstract
Background: The purpose of this study was to measure isokinetic maximum and average peak torque of internal and external rotators of glenohumeral joint in volley ball and tennis players
Methods: This study was performed on 17 professional female athletes
(7 tennis players &10 volleyball players) with age ranged 18-28 years. The subjects had played in a skilled team for more than 3 years. They were free from injury to their dominant shoulder in the past year. Subjects performed a five minute warm up by shoulder wheel and Maximum average Peak Torque (APT) were obtained unilaterally by a Biodex System 3 with the arm of players in 90 degree abduction at 120,180 & 210 o/s. Players performed five trails of concentric movements with 30 second rest between them.
Results: Maximum and average of maximum torques of shoulder rotator, in both groups, expect for internal rotators of tennis players, reduced by increase of movement speed (P<0.05). There are not significant difference between two groups in maximum, average of maximum torques and normalized data (ratio of maximum torque to weight). There is significant difference between two groups in percentage of APT of External rotator / Internal rotator ratio at 210 o/s (P<0.05).
Conclusion: Volleyball and tennis have no effect on isokinetic strength of shoulder rotators. In high speed, ratio of External rotator / Internal rotator is reduced. This indicates that increase in movement speed increase internal rotator in comparison to external rotator in these professional female athletes.
Talebian S, Olyaei Gr, Hadian Mr, Bagheri H, Mehrdad S,
Volume 65, Issue 4 (3 2007)
Abstract
Background: The changes in motor control after the use of a supportive belt can contribute to the understanding of its effects on performance. The aim of this study is to quantify motion pattern values relative to the spinal column during the use of a lumbosacral belt.
Methods: For fifty healthy female volunteers, the range of motion, maximum isometric torque and changes of phase angle of maximum torque and velocity in two positions, standing and sitting, in two directions, flexion and extension, following the use of a lumbosacral belt were measured using an isoinertial dynamometer (Isostation B200) and compared.
Results: Range of motion in both directions in both positions was reduced significantly (P<0.05). Significant reduction of maximum isometric torque was observed in flexion while sitting and in extension while standing and sitting (P<0.05). Phase angle of maximum torque and velocity also changed significantly in both directions and both positions (P<0.05).
Conclusion: The lumbosacral belt can act in different ways to prevent injury. It reduces the forces applied to lumbar vertebra and, by changing the maximum torque and velocity phase angles at the onset of range of motion, provides greater stability for joints. Furthermore, the belt can reduce stress imposed on the posterior joints of the spine and limits several momentary forces at the onset of joint movement. Under these conditions, joint injuries can be reduced and prevent some of the mechanical stress that causes lumbar joint disease.
Laal M, Granpaye L, Khodadi F, Salavatipour A, Sadeghi Ar,
Volume 66, Issue 6 (5 2008)
Abstract
Background: Various imaging tests as well as different scoring systems based on symptoms, signs and laboratory findings are commonly used to improve diagnostic accuracy in acute appendicitis. The aim of this study was to compare the diagnostic accuracy of MANTRELS scoring system (designed by Alfredo Alvarado), which is based on symptoms, signs and laboratory results, with sonographic findings.
Methods: In this prospective observational study, 106 patients with acute abdomen suspicious of acute appendicitis at Sina Hospital, Isfahan, Iran, were studied from March 2004 to February 2006.
Results: The disease was most common in patients aged 20-29 years, with male predominance. All patients had appendectomies. One hundred patients had acute appendicitis and six had negative appendectomy. Among the patients with acute appendicitis, 88% had an Alvarado score of ≥6 and 12% had a score of ≤5. Among the six patients with negative appendectomy, 66.7% had Alvarado scores of ≤5 and 33.3% had scores of ≥6, which was significantly different (p=0.005). The sonographic results were abnormal in 71.9% of patients with acute appendicitis and in 25% of patients with negative appendectomy, which was not significantly different (p=0.08). In our study, the diagnostic accuracy of an Alvarado score ≥6 was 88% and that of the sonographic results was 71.9% for acute appendicitis. If the Alvarado score ≥6 and abnormal sonographic findings are considered together, the diagnostic accuracy improves to 97.2%.
Talebian S, Olyaei Gr, Moosavi Ghomi S,
Volume 67, Issue 1 (4 2009)
Abstract
Background: The long-term effects of poliomyelitis are known in many of countries. In despite of one accrue title for these signs and symptoms there are similarity aspects in patients' problems. In the signs of explained, absence of strength and endurance, musculoskeletal difficulties, respiratory dysfunction, sleep disorders are more generalized. Prevalence of post polio syndrome (PPS) is aim of this study.
Methods: 150 subjects with history of poliomyelitis (80 male and 70 female) in Tehran city contributes in this study and complete question forms.
Results: Muscle pain was reported in 88% of subjects. Thigh muscle weakness was at 42/28%, also muscle spasm indicated at 66%. Recurrent falling of subjects appeared in 74/7%. Early fatigue reported 86%. Above five signs selected for PPS. In this study 85 subjects had four signs of above criteria or 56.66% of subjects had PPS.
Conclusion: Depended of evaluation and observation there is post polio syndrome in Tehran. Recommended for physical therapy of post polio syndrome attend to stages of progression of this syndrome. In aim to this procedure, physical treatment of these patients must limit to muscle fatigue and also severe physical and exercise activities must be reduce, also some mild aerobic activities without fatigue can be useful.
Talebian S, Olyaei Gr, Abbaszadeh M, Partabian L,
Volume 67, Issue 5 (6 2009)
Abstract
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Background: Muscle
weakness and fatigue are two factors that cause muscle injuries. The roll of
motor control is important in regulation and reduction of this effect.
Repetition of motion after muscle fatigue is due to the significant role of
motor control system. The aim of this study was to evaluate the quality of roll
control of central nervous system following fatigue in order to answer primary
questions in therapeutic exercises.
Methods: Twenty voluntary healthy subjects
participated in this study. Dominant abductor policis brevis after surface
electromyography (SEMG) of maximal voluntary contraction
(MVC) fatigued by maximum three
minutes isometric contraction in specific hand splint monitored by dynamometer
until reduction of activities to 50% MVC. Before and immediately after
fatigue test, 5 dynamic full range of motion in
abduction direction performed without resistance and muscle activities recorded
by SEMG. Three middle muscle activities
selected for comparison of Symmetrical Index (SI) as specificity of motor control assessment. Fatigue
indicators were percentage of reduction of MVC and median and mean frequency of power spectrum of MVC before and after fatigue test.
Results: Significant
reduction of MVC (46.77%), median (13.03%) and
mean (12.20%) frequency were showed (p=0.001),
whereas significant increase of SI (10.76%) appeared (p=0.000),
following fatigue test.
Conclusion: Fatigue reduces muscle
contraction velocity, therefore median and mean frequency decrease. Also after
muscle fatigue reduces of muscle proprioception sense appears, that can cause
slow dynamic joint motion. Upper central control systems try to correct this
situation by involving new motor units and changes of muscle timing.
Synchronization of primary and new motor units plays an important role in
increase of SI.
Mortezaeian Langroodi H, Rad Goodarzi M, Nakhostin Davari P, Shahmohammadi Aa, Mearaji Sm, Aarabi Moghadam My,
Volume 68, Issue 6 (6 2010)
Abstract
Background: Coarctation of aorta is narrowing of proximal descending aorta. Interventional procedures such as balloon angioplasty & stent implantation has been progressively in use as alternatives for surgery in increasing number of children with diagnosis of coarctation of aorta. The aim of this study was to evaluate the use of endovascular stent in children with coarctation of aorta.
Methods: We evaluated effectiveness and safety of stenting in all patients younger than 18 years old with coarctation and re-coarctation of aorta which treated by stenting between years 2004-9 at rajaei - heart centre in Tehran, Iran.
Results: we studied 53 patients younger than 18 years old with a follow up of six months. Totally 54 stents were implanted. Mean (±SD) age of the patients was 11.6±4.2yrs. Seventeen cases (32%) were younger than 10 years old, and 36 cases (68%) were 10 years and older. Mean (±SD) weight was 39.24±18kg. 16 cases weighting less than 25kg. Peak systolic pressure gradient (SPG) decreased from 46.26±17.07 to 1.03±0.19mmHg after procedure (p<0.001). There was no significant difference (p<0.001) in the gradient before and after stenting in the patients with native coarctation (Vs re-coarctation cases), less than 25 kg and under 10 years old groups. Complications developed in 44% of cases while dominantly were minor except in two cases re-dilatation of stent was not needed during six month of follow-up of the patients.
Conclusions: Stenting of coarctation of aorta can decrease complications and can be used safely in children weighing bellow 25kg and in children below 10yrs old.
Hashemi Sj, Zaini F, Charsizadeh A, Daiedaie Ghazvini R, Grami Shoar M,
Volume 69, Issue 1 (4 2011)
Abstract
Background: Infections caused by opportunistic yeasts such as Candida species,
Trichosporon, Rhodotorula and Saccharomyces have increased in immunocompromis- ed patients and their identification is crucial as intrinsic and acquired resistance of some yeast species to antifungal agents are on the rise. The aim of this study was to identify the organisms to the species level in order to suggest accurate and effective antifungal therapies.
Methods: In this study that carried out in Tehran, Iran in 2009, 200 patients with yeast infection were medically examined and clinical specimens were prepared for direct examination and culture on Sabouraud dextrose agar. Subsequently, the isolated yeast
colonies were identified using various tests including culture on Corn Meal agar with
Tween 80, CHROMagar Candida and casein agar. For the definite identification of organisms some biochemical tests were done based on carbohydrate assimilation by RapID Yeast Plus System kit, and, finally, a molecular method, PCR-RFLP, using Hpa II enzyme, was performed for the remaining unknown yeast species.
Results: A total of 211 yeast isolates were identified in 200 patients with yeast infections. The most frequent isolated yeasts were Candida albicans, 124 (58.77%), followed by Candida parapsilosis, 36 (17.06%), Candida tropicalis, 17 (8.06%),
Candida glabrata, 13 (6.16%), Candida krusei, 8 (3.79%), Candida guilliermondii, 2
(0.96%), Trichosporon, 3 (1.14%), Rhodotorula, 1 (0.47%), Saccaromyces cerevisiae, 1 (0.47%) and other yeast species, 6 (2.84%).
Conclusion: Nail candidiasis was the most prevalent type of yeast infection in the patients and Candida albicans was the most frequent isolated species from all clinical specimens.
Nakhostin Davari P, Mortazaeian Langrodi H, Ghaemi Hr,
Volume 69, Issue 8 (6 2011)
Abstract
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Background: Isolated
pulmonary valve stenosis represents 8-10% of congenital cardiac anomalies. This study was performed to
evaluate the late outcomes of Balloon Pulmonary Valvuloplasty (BPV) in children with residual
infundibular stenosis.
Methods : Seventy-eight patients underwent BPV
in Rajaee Heart Center in Tehran Iran, from
2008-2010.
The patients were divided into two groups: with and without infundibular
stenosis. The group with infundibular stenosis was subdivided into two groups:
with and without propranolol administration. Gradient measurement follow-ups by
Doppler echocardiography were done on the first day and 1, 3, 6 and 12 months afterwards.
Results: Thirty five (44.9%) patients were male and
43 (55.1%)
were female. The mean age of participants was 4.29±3.5 years and the mean weight 16.18±8.8
kg 33 patients (43%) did not show residual infundibular stenosis but 45 (57%) did so in the
evaluations. There were no significant differences between the two groups
regarding age, weight, sex, kind of balloon valvuplasty, balloon to body
surface area ratio and pulmonary regurgitation (PR). Propranolol was administered to 27
(60%) patients with residual stenosis for six
months but 18 (40%) did not receive the medication. In both groups, the gradient
significantly decreased immediately after BPV
(P<0.0001). In the propranolol group a constant
decrease in residual gradient was seen. There was a significant relationship
between balloon to body surface area ratio (387±94mm2/m2) with moderate to severe PR (P<0.015).
Conclusion: BPV is a safe and effective procedure
to treat PS. Residual infundibular gradient after BPV decreases over time and
propranolol helps decrease the gradient.
Khalili Gr, Sajedi P, Danesh H,
Volume 69, Issue 11 (4 2012)
Abstract
Background: Emergence agitation (EA) is a post-anesthetic problem which interferes with a child's recovery and presents a challenge in terms of assessment and management. In this study, we compared the effects of midazolam and ketamine as premedication in the management of EA in children aged 1-6 years.
Methods: In this prospective, randomized clinical trial study, 58 children aged 1-6 years who were undergoing general anesthesia for elective surgery in Alzahra Hospital in Isfahan during 2008 until 2009. The patients were randomly assigned to receive 0.1 mg/kg midozolam (28) or 0.5 mg/kg ketamine (29) by IV route in the premedication room. All patients received a standardized anesthetic regimen and isoflurane was used for the maintenance of anesthesia. The incidence and severity of agitation (agitation score), severity of pain (pain score), anesthesia, recovery and extubation durations were recorded postoperatively.
Results: The prevalence of agitation in midazolam (21.4%) was lower than ketamine group (34.5% P<0.05). In addition, the duration of agitation in ketamine group (21±16.67 min) was significantly higher than midazolam group (6.83±6.55 min), (P<0.05). However, no significant differences were seen in agitation score, pain score, anesthesia, recovery or extubation durations in the two groups (P>0.05).
Conclusion: The study showed that midazolam could reduce the frequency of agitation better than ketamine but both drugs were able to reduce the severity of agitation after short-time surgeries in young children.
Heydari Sv, Ramak Hashemi Sm, Abasnejad Ea, Abbasi Gravnd F, Ghorbani Yekta B,
Volume 70, Issue 5 (5 2012)
Abstract
Background: The purpose of this study was to determine the prevalence and associated factors for postoperative pulmonary complications (PPCs) and extubation failure in patients having undergone intracranial surgery.
Methods: In this retrospective study done in Firozgar Hospital during 2008-2010, we followed up 254 patients through a clinical questionnaire and observation of the clinical course of participants in pre- and post-operative periods.
Results: Overall, 40 (15.74%) patients had postoperative pulmonary complications. The most common PPC was pneumonia, which was seen in 24 patients (60% of complications). The average duration of ventilation in patients with PPC was significantly higher (16.8.±10 vs. 5.09±4.5 days P=0.001) than patients without the complication. The mean Glasgow coma scale (GCS) after surgery in patients with PPC was significantly lower (11.±4 vs. 13.2±3 P=0.001) than the rest of the patients. Moreover, the mean age of patients with PPC was significantly higher (64.02±14 vs. 41.6.±17 years P=0.001). Average duration of stay in ICU in patients with PPC was also higher (24.±27 vs. 8.7±0.5 P=0.001).
Conclusion: GCS before surgery and failed extubation independently of other variables were significantly associated with pulmonary complications independent of other variables.
Sattarzadeh Badkoobeh R, Derakhshan L, Farhang Zand Parsa A, Pasha Meysamie A, Khosravi B, Grayly B, Jabari Sm,
Volume 70, Issue 8 (5 2012)
Abstract
Background: Measurements of mitral valve area (MVA) are essential to determine the severity of mitral stenosis (MS) and adopt the best management strategies. The aim of the present study was to compare MVA determined by two-dimensional (2D) planimetry to MVA measured by continuity equation (CE) in patients with moderate to severe MS.
Methods: We evaluated 73 consecutive patients with the diagnosis of MS scheduled for balloon mitral valvuloplasty or with moderate to severe rheumatic MS admitted at the echocardiography clinic of Imam Khomeini Hospital in 2010. Using 2D images of mitral valve obtained from paraesternal short axis view, 2D planimetry of the mitral orifice area was performed by an experienced cardiologist. MVA by CE was calculated from aortic forward stroke volume divided by transmitral time-velocity integral.
Results: The mean value of MVA by 2-D planimetry was 1.0±0.3 cm2. The average values of MVA measured by PHT and CE were 1.0±0.3 cm2 and 0.9±0.4 cm2, respectively. The MVA determined by planimetry correlated well with CE (r=0.832, standard error of estimation [SEE]= 0.166, P<0.001). The mean values of MVA calculated by CE highly correlated with those calculated by 2-D planimetry in patients presenting with both non-significant (r=0.701) and significant (r=0.761) AIs.
Conclusion: When planimetry is not feasible, such as in severe calcification of mitral valve or after percutaneous balloon valvuloplasty, CE could be an alternative method for MVA measurement in comparison with PHT.
Karambaksh A, Noori Mougahi Smh, Hassan Zadeh Gr, Tak Zaree N,
Volume 70, Issue 10 (4 2013)
Abstract
Background: Nitric oxide (NO) is produced in different body organs in mammals and numerous physiological and pathological properties are attributed to this small molecule. The precursor of this substance in the body, L-arginine, is synthesized by the enzyme nitric oxide synthase (NOS), and it is catalyzed, and is inhibited by a substance called L-NG-nitroarginine methyl ester (L-NAME). In this study we investigated the qualitative and quantitative effects of nitric oxide on cerebellar histopathology in vivo environment via increasing and decreasing its production.
Methods: Forty Wister rats, weighing 200- 250 gr with a mean age of 8 weeks, were divided into 5 groups after making sure the rats were pregnant. Except the control group, the other pregnant groups, respectively received: 2 ml/kg normal saline, 200 mg/kg L-arginine, 20 mg/kg L-NAME and a mixture of the same doses of L-arginine and L-NAME on the third, fourth and fifth days of pregnancy. On day 18 of pregnancy, we anesthetized the rats, excised the cerebellum after craniotomy and fixed the organs in 10% formalin. We later prepared 5 to 6-micron in thickness tissue sections and dyed them by the routine Hematoxylin and eosin (HE) and Masson's Trichrom staining methods before studying them by light microscopy.
Results: There was a significant difference between the rats receiving L-arginine and the rats in other groups (P<0.01).
Conclusion: This study showed that L-NAME is capable of significantly decreasing the injury caused by nitric oxides in rat cerebellum.
Razieh Zarifian Yeganeh , Abbas Shakoori Garakani , Saman Mehrabi , Nader Ebadi, Maziar Motiee Langroudi , Mohammad Reza Noori Daloii,
Volume 75, Issue 7 (October 2017)
Abstract
Background: Head and neck squamous cell carcinoma (HNSCC) is the malignancy of squamous cells (the epidermal layer of skin) in cavities in head and neck includes: larynx, pharynx, paranasal sinuses and oral cavity. The main goal of this research was to understand the effect of mutations in two important genes (KRAS and BRAF) in RAS/MAP kinase (EGFR) signaling pathway in tumor cells with head and neck squamous cell carcinoma in Iran.
Methods: The present cross-sectional study performed from October 2015 to September 2016 on 40 patients suffering from head and neck squamous cell carcinoma, all confirmed by pathology department of Imam Khomeini hospital. Tumor samples were achieved from the surgical cancer department of Imam Khomeini hospital and stored in liquid nitrogen until starting tests. The tests done in genetic laboratory of Tehran University of Medical Sciences. Techniques we used in this research, were DNA extraction based on phenol-chloroform approach, Multiplex PCR (M-PCR) to amplify mentioned exons and KRAS/BRAF strip assays to detect mutations in mutated hotspots in exon 2 of KRAS and codon V600E in BRAF gene.
Results: In this study, we observed 7 mutations in codons 12 and 13 exon 2 in KRAS gene (about 17.5%) and 4 mutations in codon V600E in BRAF gene (about 10%) of obtained tumor samples. The hotspot mutation in codon 12 were Asp (10%) and Ser (5%) respectively. In BRAF, the most common mutation, as we expected according to other researches, was observed in codon V600E. We also observed that 29 people of these patients were male (about 72.5%) and 11 patients were female (about 27.5%). Moreover, 28 patients were over 50 years, while 7 patients were below the age of 50.
Conclusion: The results of this study showed that mutations in genes KRAS and BRAF especially in studied hotspots, and the effects on their molecules in EGFR signaling pathway are important in involving head and neck squamous cell carcinoma, as other cancers. These findings may be considered in choosing drugs for targeted chemotherapy.
Isa Khaheshi, Taraneh Faghihi Langroudi , Sima Salimi, Marjaneh Karimi, Abbas Arjmand Shabestari , Maral Edalati, Shahabeddin Gorji, Elham Mahmoudi ,
Volume 78, Issue 4 (July 2020)
Abstract
Background: Systemic sclerosis is an autoimmune disease affecting connective tissues, (including epidermal, subepidermal, microvasculature, etc.), leading to various extent of end-organ damage. The leading cause of mortality among these patients is lung involvement. The cardiovascular events happen more frequently in patients suffering systemic scleroderma, comparing to healthy population. This study was designed to clear the correlation between development of coronary calcification (as an indicator of atherosclerosis) and lung disease in these patients.
Methods: All patients with definite diagnosis of systemic sclerosis, who referred to Shahid Modarres Hospital between March 2011and March 2014, entered to the study. Patients suffering hypertension, hyperlipidemia and who had a past or current history of smoking were excluded from the study. Atherosclerosis was determined by coronary calcium score (based on Agatston score) and the severity and extent of lung disease was assessed by wells scoring system and Warrick scoring system (based on lung CT scan without contrast). The spearman correlation analysis was done on the data by SPSS software, version 20 (IBM SPSS, Armonk, NY, USA). All the patients had informed consent and no additive charge was delivered.
Results: The study population consisted of 25 patients with systemic sclerosis. 21 patients were female and the 4 other ones were male. The mean age was 67±4 years old. The mean Wells score in patients was 13±2.59, the mean Warrick severity score was 6.54±6.16 and the mean Warrick extent score was 14.42±14.59. No correlation was observed between presence of calcification in coronary arteries (Wells score r2=0.63 P=0.77, severity score r2=0.27, P=0.2, extent score r2=0.11, P=0.6), aorta annulus (Wells score r2=0.04, P=0.83, severity score r2=0.06, P=0.77, extent score r2=0.06, P=0.76) and thoracic aorta (Wells score r2=0.05, P=0.83, severity score r2=0.03, P=0.9, extent score r2=0.03, P=0.9) with the severity and extent of lung involvement.
Conclusion: It seems that the presence of coronary atherosclerosis or calcifications in aorta annulus and thoracic aorta (assessed by Agatston calcium score) has no significant correlation with the severity and extent of lung disease (assessed by Wells and Warrick score) in patients with systemic sclerosis. |
Arash Bostani, Hadi Gharabaghian Azar, Mehdi Jafari, Mastane Babaei Gramkhani,
Volume 80, Issue 1 (April 2022)
Abstract
Background: Multiple sclerosis (MS), as an inflammatory autoimmune disease and chronic degenerative central nervous system degeneration, often occurs in early adulthood. One of the common and debilitating symptoms of this disease is fatigue, which can affect up to 80% of patients with MS. This study aimed to evaluate the effectiveness of magnetic field therapy on fatigue in patients with MS.
Methods: Present study is a single-blind randomized clinical trial (RCT) that Was conducted on patients with multiple sclerosis from March 2019 to September 2021. In this study, 46 patients who met our inclusion criteria were divided into two groups randomly: magnetotherapy intervention and control group. The intervention was performed in the form of a pulsed magnetic field with a frequency of 15 Hz and an intensity of 4.5 Millie Tesla. In order to blind patients, they were unaware of the intervention or control group, and the technician in charge of the treatment and the statistician knew about it. Data were then collected and recorded using the Fatigue Severity Scale, the Patient Health Questionnaire, and the Epworth Sleepiness Scale Questionnaire.
Results: Of the 46 patients studied, 22 (48%) were male and the rest of them were female [24 cases (52%)]. The mean age of men and women was 34.4±7.3 and 33.5±1.7 years, respectively. Among the intervention and control groups, 8 people took amantadine equally in each group. In the magneto-therapy intervention group, the mean severity of fatigue reduced from 4.91±0.86 to 4.27±1.10, which was significant (p=0.024). In our control group, the mean intensity of fatigue reduced from 4.83±0.83 to 4.37±0.81, which was significant statistically (p=0.028). Although, the difference between the response of the two groups to treatment was not significant (p=0.382).
Conclusion: Due to the lack of significant differences in the reduction of fatigue severity, this treatment is not recommended for the treatment and management of fatigue in patients with MS.
