Search published articles


Showing 2 results for Khanehzad
The roles of Sertoli cells in fate determinations of spermatogonial stem cells
Maryam Khanehzad , Farid Abolhasani , Seyed Morteza Koruji , Iraj Ragerdi Kashani , Fereshteh Aliakbari ,
Volume 73, Issue 12 (March 2016)
Abstract

Background: Spermatogenesis is a complex and highly organized process of proliferation and differentiation of spermatogonial stem cells. Spermatogonial stem cells (SSCs) as a unique stem cell have the potential to self-renewal, differentiation and transmit genetic information to the next generation and play a vital role in maintaining fertility. Sertoli cells as the only somatic cells within the seminiferous epithelium play central roles in the formation of niche and balance between self-renewal and differentiation by secrete many growth factors. Given the importance and widespread use of SSCs, particularly in the treatment of infertility, the aim of this study was to create an optimal environment for the proliferation of SSCs. So we decided to study of undifferentiated (ID4) and differentiated (c-Kit) gene expression in SSCs followed by co-culture with Sertoli cells for a one-month.

Methods: This experimental study was conducted from November 2013 to December 2014 in Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, on immature NMRI mouse (6-3 days old). Initially, Sertoli cells and SSCs were isolated from neonates mouse testes during the two-step enzymatic digestion characteristics Sertoli cells with vimentin marker and SSCs with promyelocytic leukemia zinc-finger (PLZF) marker were confirmed. Then SSCs were cultured in two groups: co-culture with Sertoli and without co-culture (control). Undifferentiated (ID4) and differentiation (c-Kit) gene expression were evaluated by Real-time PCR technique.

Results: Spermatogonial stem cells purity was obtained 66.91% by flow cytometry. The relative expression levels of gene ID4 in co-culture group at the end of each week, compared to the control group showed a significant increase (P<0.05). While the expression of this gene significantly decreased in each group over time (P<0.05). The results of the comparison of the relative expression of c-Kit gene in co-culture group are indicated significant decrease than the control group at the end of each week (P<0.05). In addition, this gene expression was showed significant increase in each group individually over time (P<0.05) ID4 gene expression showed a significant (P<0.05) increase toward the control group, while in the expression of c-Kit was observed a significant (P<0.05) decrease compared with the control group at the end of each week.

Conclusion: According to the results of this study, co-culture with Sertoli cells maintains SSCs in the prolifration stage for long-term, so can be used to optimize the culture medium at the clinic.


Stature estimation by lower limb anthropometry in Iranian medical students
Shokoofeh Kazemzadeh, Maryam Khanehzad, Sina Mojaverrostami, Soheila Madadi, Tahereh Alizamir,
Volume 82, Issue 8 (November 2024)
Abstract
Background: Estimating the stature and identifying unknown bodies is an important challenge for forensic specialists and anthropologists, especially when dismembered and mutilated body parts are found. The purpose of this study was to predict the stature by measuring lower limb parameters and to determine the relationship between stature and thigh length, leg length, foot length and lower limb length in Iranian medical students.
Methods: This study was conducted in the Department of Anatomy, Tehran University of Medical Sciences, Tehran, Iran in 2019 (May-August). The study population consisted of 200 Iranian medical students (100 males and 100 females, aged 17-36 years). Four lower extremity parameters (thigh length, leg length, foot length, and lower limb length) and stature were measured on the left side according to standard measurement methods. All measurements were performed according to ethical standards provided by the Ethics Committee for Human Experimentations. The measurements were done using standard anthropometric instruments. Linear regression analysis was used to investigate the relationship between the stature and the lengths of the four lower extremity parameters. Then, stature prediction was done based on regression equations. The data were analyzed using SPSS version 25. Independent-samples t-test was used to compare the mean values of the anthropometric measurements between males and females. The relationships between quantitative data were evaluated using the Pearson’s correlation coefficient and linear regression was applied to determine the relationship between stature and lower extremity parameters.
Results: The results of this study indicated a strong correlation between stature and lower limb length (r=0.892, P=0.000), foot length (r=0.852, P=0.000) and thigh length (r=0.805, P=0.000) and a moderate correlation between stature and leg length (r=0.505, P=0.000).
Conclusion: The results of this study suggest that lower limb dimensions and regression equations are appropriate for stature estimation in forensic medicine. However in the future, further research should be conducted to analyze the applicability of these equations for stature estimation in other Iranian populations.

Page 1 from 1     

© 2026 , Tehran University of Medical Sciences, CC BY-NC 4.0

Designed & Developed by : Yektaweb