Rohani M, Khorshidi A, Moniri R, Torfeh M, Abddoshah F, Saffari M, Shajari Gh R, Moosavi Gh A,
Volume 67, Issue 3 (5 2009)
Abstract
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Background: Tuberculosis is an important cause of death
in some countries. The world health organization estimates that if stronger
measures are not taken up to control the prevalence of this disease, from 2000 to 2020 a billion people will be infected by the
bacterium. According to time consuming of common detection methods of Mycobact-erium
tuberculosis such as culture, it is necessary to evaluate a rapid detection
tests such as PCR. Rapid diagnosis of tuberculosis may have
profound effects in patients' care According to importance of rapid detection
and treatment of tuberculosis and for determine of sensitivity, specificity,
positive predictive value and negative predictive value of PCR by using IS6110 this study was done
in Kashan university of medical science.
Methods: A total of 248
sputum samples from patients suspected of mycobacterial diseases were studied. DNA was extracted by boiling method. IS6110 PCR method by a specific pair of primers designed to amplify 123bp and 245bp sequences of the insertion sequence, 6110, in the M. tuberculosis genome was used to
analyze sputum samples.
Results: 32 out of 248 (12.9%) of samples had positive culture. PCR yielded a sensitivity of 93.8% and
specificity of 99.1% for the diagnosis of TB patients with TB confirmed by culture. There were two out
of 32 (6.3%) PCR-positive cases
among the patients with non-TB disease.
Conclusion: The findings of the present study indicate that
Multiplex PCR
may provide a faster method of detecting tuberculosis, thus enhancing
diagnostic procedures and we conclude that the performance of an IS6110 PCR assay is valuable in the rapid diagnosis of
tuberculosis.
Khadije Sohrabi, Abbas Ali Gaeini , Elham Shirzad , Shahram Khorshidi , Shahriar Nafissi, Hamid Reza Fateh,
Volume 82, Issue 4 (July 2024)
Abstract
Background: Spinal Muscular Atrophy (SMA) is a neurodegenerative disorder caused by a mutation in the survival motor neuron 1 (SMN1) gene. It is classified into five types (from type 0 to 4) based on the age of onset of symptoms and maximum motor function. This autosomal recessive mutation results in progressive weakness and atrophy of the proximal muscles. Due to the high cost of treatment, the critical timing of intervention, and the varied responses of patients, many individuals do not sufficiently benefit from current therapeutic methods. This study evaluates the impact of resistance training on the quality of life and fatigue in patients with SMA type III, considering the potential benefits of such training.
Methods: The present study was developmental and semi-experimental. Fourteen ambulatory patients with SMA type III were randomly assigned to exercise and control groups. The exercise group engaged in lower limb resistance training at an intensity of 6 to 8 on an adult resistance exercise scale for 10 weeks (25 sessions). During this period, the control group maintained their usual daily activities. Patients in the exercise group did not engage in any exercise activity other than the protocol of the present study. Patients' quality of life was measured with the Short Form 36 (SF-36) questionnaire, and fatigue severity was measured with the Fatigue Severity Scale (FSS), both evaluated pre- and post-intervention. The assessments and exercises were conducted in the occupational therapy department of Shariati Hospital, Tehran, from July 2023 to September 2023.
Results: Analysis of the results showed significant improvements (P<0.05) in the exercise group compared to the control group in the subscales of physical functioning and fatigue within the quality of life assessment. Additionally, notable differences were found between the groups on the fatigue intensity scale. However, No significant difference was observed in the patients' Body Mass Index (BMI) measurements.
Conclusion: Progressive resistance training of the lower limbs enhances the quality of life and reduces fatigue in patients with SMA type III. |
