Gholampour Azizi I, Khoushnevis S H, Hashemi S J,
Volume 65, Issue 13 (Vol 65, Supplement 1 2008)
Abstract
Background: Aflatoxins are severe toxic secondary metabolites found in most plant
products. When animals consume contaminated feed stuff to Aflatoxin B1 (AFB1), the
toxin is metabolized by liver and is excreted as Aflatoxin M1 (AFM1) via milk. Aflatoxins
are acute toxic compounds, immunosuppressive, mutagen, tratogen and carcinogen.
Methods: During the winter of 2006, pasteurized and sterilized (ultra high temperature)
(UHT) milk packages were collected from supermarkets in Babol city. 78 pasteurized and
33 sterilized milk, totally 111 samples were tested for AFM1 by competitive Enzyme
Linked Immunosorbent Assay (ELISA). Solid phase in plastic micro wells coated whit
anti-Aflatoxin M1 antibodies. We added 100 microliter skimmed milk and Aflatoxin M1
standard solutions in each well. In each plate, we appointed seven wells for standards.
Plates were incubated at 20-25° centigrade for 45 min. Each well was washed four times
by washing buffer 20X concentration. Then 100 micro liter conjugated solution (100X)
was added to each well, and the plate was incubated at 20-25 centigrade for 15 min. After
that, the wells were washed. After adding the substrates to wells, we incubated the plate
at 20-25° centigrade in a dark place for 15 min. The reaction was stopped by stop solution.
After one hour, light absorption was read at 450 nm by ELISA reader.
Results: AFM1 were detected in 100% of all samples. 100% of samples were above of
European community regulations (50ng/l). AFM1 contamination mean levels pasteurized
and sterilized milk were 230.5 and 221.66 respectively. Therefore more than four fold
levels European community. There is not a significant relationship between AFM1
contamina-tion level and different months of winter applying statistical test.
Conclusion: The results showed the need for introducing safety limits for AFM1 levels in
child milk under Food Legislative liable of Iran. Aflatoxin M1 contamination is a serious
problem for public health, and it is potentially hazardous for human health.
