Showing 2 results for Poor Fath
M Shayegan, A.a Poor Fath , M Nomeiri, Gh Babaei, F.a Tarabadi,
Volume 62, Issue 4 (11 2004)
Abstract
Background: As increase of cytokines (like IL-1 , IL-6 , IL-8 and TNF- ) in platelet concentrates (PCs) during storage are involved in Febrile Non Hemolytic Transfusion Reactions (FNHTRs) after Platelet transfusion, the aim of this study was the survey of these cytokines level in PCs produced in Tehran Blood Center. Materials and Methods: This study proposed to determine if WBC reduction in PCs by pre-storage leuckodepletion filters reduced the levels of these cytokines during storage uo 3 days. Each of the PCs (n = 54) was prepared from single random donor (RD) by Platelet-Rich-Plasma (PRP), then were divided in four groups: 1- unfiltered non-irradiated RD-PCs (n= 13) 2- unfiltered and -irradiated RD-PCs (n=16) 3 - filtered non-irradiated RD-PCs (n=14) 4-filtered and -irradiated RD-PCs (n = 11). Cytokines levels in PCs supernatants were measured by ELISA kits according manufacture‘s instructions. Results: Our results showed : IL-8 was increased in unfiltered non-irradiated and IL-18 in -irradiated RD-PCs but not in the filtered RD-PCs in day 3 . Compared to unfiltered PCs in filtered units, pre-storage filtration prevented a rise in the IL-8 and TNF- in day 3 of storage .The concentration of IL-1 was lower than the minimal detectable concentration by the kits used for this purpose. IL-6 was detected only in 7 units of all filtered PCs in day 3. Conclusion: These data indicate that pre-storage leuko-reduction of PCs can prevent accumulation of IL-6, IL-8 and TNF- during storage.
Shayegan M, Poor Fathollah A.a, Namiri M, Babaei Gh,
Volume 63, Issue 4 (13 2005)
Abstract
Background: Tumor necrosis factor alpha (TNF-) is a major mediator of inflammatory responses and also plays a role in Febrile non-hemolytic reactions (FNHRs) after platelet concentrates (PCs) injection. It is thought contaminating WBCs are the source of these cytokine so inactivating or decreasing of these WBCs will be effective to decline of the cytokines. Due to difference results between different methods for TNF measurement, in this study we compared Immunoassay and Bioassay methods to determine TNF- in supernatants of PCs.
Materials and Methods: TNF- was measured by ELISA (Immunoassay) and by the L929 cytotoxicity Bioassay in supernatant of random donor PCs (RD-PC) prepared by platelet rich plasma (PRP). These platelet concentrates were divided in 4 groups: 1- unfiltered , non-irradiated RD-PCs (N=13) 2- unfiltered and -irradiated RD-PCs (N=16) 3- filtered , non-irradiated RD-PCs (N=14) 4- filtered and -irradiated RD-PCs (N=11)
Results: Our results showed: • TNF- was increased (by ELISA) in unfiltered non-irradiated units during storage but not in -irradiated and filtered RD-PCs in day 3. Compared to unfiltered PCs in filtered units, pre-storage filtration and irradiation prevented a rise in the TNF- in day 3 of storage. • There was no correlation between ELISA and the cytotoxicity L929 bioassay .
Conclusion: It has been previously reported that different quantitative results can be obtained when TNF alpha is measured in biological fluids by Bioassay and Immunoassay. This is thought to be related to the presence of antigenic forms of TNF alpha that cannot be detected by bioassay, such as complexes with soluble receptors (sTNF-R) or TNF alpha monomers.
