Showing 6 results for SMH
M Karimfar , Smh Nuri , B Minaiy , M Barbarestani ,
Volume 56, Issue 2 (30 1998)
Abstract
To study the lead effect, we selected 14 females rats in which we had observed vaginal plague after mating, and divided them into a group of 8 female rats as the case group and 6 females as the control group. As drinking water the case group was given 0.13% lead acetate solution and the control group pure distilled water. Immediately after parturition the pups were killed and their livers removed. A small part of the liver was fixed in glutaraldehyde and osmium tetroxide in order to be studied with transmission electron microsope. The fixated parts were dehydrated and embed in Epon 812. using ultramicrotomoe, we prepared semithin seclions of the tissues, stained them with toludine blue, and studied them under light microscope. Further more we prepared thin sections of the tissue to be placed on grids coated with formvar. These sections were stained by uranyl acelate and lead citrate. Electron microscopic study was performed on them. Results: T.E.M. study showed the swelling and blebbing of nuclear envelop and endoplasmic reticulum and mithochondria of hepatocytes.
Smh Nuri , B Minaiy , M Shamshiri , J Anarluki ,
Volume 56, Issue 2 (30 1998)
Abstract
With the intention of research of various methods of epithelial tissue culture we've studied five French Albino rabbits with an average of 8 weeks. In order to evaluate and control growth and proliferation of autologus cultured tissue samples were obtained on 1st, 5th and 8th days. After fixation of these samples and passing them through various processes, histologic sections were prepared. These sections were stained with H-E and studied by light microscope, we succeeded in developing the original donor surface by 18 times.
Mahmoodzadeh Sagheb Hr, Dezfoulian A, Noori Smh, Heidari Z, Chitnis P ,
Volume 60, Issue 2 (14 2002)
Abstract
Background: Stereologic methods are used to obtain quantitative information about three dimensional structures from histologic sections. The aim of present study was using new and unbiased stereological techniques to investigated changes in volume and number of glomeruli after chronic lead acetate intoxication. Lead is one of the heavy metals that have adverse effects on renal function. Its effects can involve both renal tubules as well as glomeruli. So many studies based on observation and qualitative reports. Some of which report changes in volume and number of glomeruli.
Materials and Methods: Male Wistar rats were randomly divided to four groups (n=9). During a period of 8 weeks, the treatment groups were given 0.5 percent and 1 percent lead acetate in drinking water and the control and sham control were given distilled water and 0.4 percent acetic acid solution respectively. Stereological analysis based on Cavalierie's principle was performed to determine the reference volume (VRefrence), the fraction volume of glomeruli (VVGlom) and total glomerular volume (VTGlom). Furthermore, for estimating the numerical density (NVGlom) and total number of glomeruli (NTGlom), the physical dissector was utilized.
Results: Results showed that the number of glomeruli in treatment group which received 1 percent lead acetate in drinking water decreased significantly (P<0.05), but no changes occurred in 0.5 percent group (P>0.05). On the other hand glomerular total volume in both 0.5 percent and 1 percent groups increased significantly in comparison to control and sham control groups (P=0.000).
Conclusion: This experiment is in agreement with other qualitative reports by using unbiased method of stereological methods and showed changes in volume and number of glomeruli following lead acetate intoxication.
Afsarian Smh, Zaini F, Kordbacheh P, Mahmoudi M, Rezaii S, Safara M,
Volume 64, Issue 12 (6 2006)
Abstract
Background: I Infections due to Candida spp. have increased dramatically in recent years through a rising number of predisposing factors and immunocompromised hosts. Although Candida albicans is the most prevalent and important causative agent of Candida infections, the importance of C. parapsilosis, C. tropicalis, C. krusei, C. glabrata, C. guilliermondii and C. kefyr have increased significantly as they tend to be more resistant to antifungal agents. Therefore, it is critical that infecting Candida spp. be identified and considered. Furthermore, clinical laboratories may need to expand their yeast identification capabilities in order to facilitate rapid identification of clinical yeast isolates.
Methods: In a discroptive – analytic study, the patients suspected of candidiasis were sampled. Direct examination and culture was carried out for all specimens. The isolated yeast colonies were then identified using various different tests such as culture on corn mealagar tween-80, CHROMagar Candida, and assimilation test by API 20C AUX kit.
Results: In the present study, 304 yeast colonies were isolated from referral patients to mycology laboratory of 304 isolated colonies 204 were identified as C. albicans and 100 were identified as non albicans candida as follow 35% C. parapsilosis, 32% C. tropicalis, 8% C. glabrata, 8% C. kefyer, 6% C. krusei, 3% C. guilliermondii, 3% C. famata, 3% C. lusitaniae, 1% C. zeilanoides and 1% C. homicola. C. parapsilosis was the most frequent species. The result showed that clinical specimens were obtained from various infected sites of body and nail samples (59 cases) were found to be the most frequent among those specimens.
Conclusion: In conclusion, our results suggest that no single phenotypic test has proven to be highly effective for definitive identification. Moreover since these organisms can vary greatly in their susceptibility to the current antifungal agent and causing significant patient management problem therefore evaluation of susceptibility of these isolates against antifungal drugs is need to be investigated.
Rafiei M, Sadr Bafghi Smh, Nasirian M, Namayandeh Sm, Abdoli Am, Sadr Bafghi Sm,
Volume 66, Issue 7 (6 2008)
Abstract
Normal
0
false
false
false
EN-US
X-NONE
AR-SA
MicrosoftInternetExplorer4 !mso]>
ject classid="clsid:38481807-CA0E-42D2-BF39-B33AF135CC4D" id=ieooui>
Background: Atherosclerosis and the side effects thereof
are a major cause of mortality and morbidity in diabetic patients. Diabetic
dyslipidemia is defined by a decrease in blood levels of HDL cholesterol and increases
in triglycerides and LDL
cholesterol. Diabetic dyslipidemia is
atherogenic, inducing cardiovascular disease in diabetic patients at a
frequency that is two to three times greater than that of nondiabetics.
Methods: This study analyzes
the data from the first phase of the Yazd Healthy Heart Program, a community
intervention project focused on the prevention of cardiovascular disease. Using
the cluster sampling method, we analyzed data from 2000 subjects from Yazd, Iran,
ranging in age from 20
to 74 years. Clinical and
paraclinical data were recorded by trained health providers using a
questionnaire with over 700
items.
Results: The most frequent
lipid disturbance was TG>150 in 67.1% of the type-II diabetic patients (p<0.000), 54.6% of whom were unaware
of their TG level. The mean lipid
and TG levels are
significantly higher (p<0.000). Furthermore, women as a group have higher mean cholesterol, LDL-C and HDL-C levels than men (p<0.000). Additionally, diabetic patients were more obese than the
nondiabetic population. (p<0.000).
Conclusion: The high prevalence of
diabetes mellitus in Yazd and hypertriglyceride-mia among diabetics in this
city, in addition to the lack of awareness among more than half of these patients
about their illness and diagnosis, indicate an urgent need to immediately
control dyslipidemia in these high-risk patients.
Karambaksh A, Noori Mougahi Smh, Hassan Zadeh Gr, Tak Zaree N,
Volume 70, Issue 10 (4 2013)
Abstract
Background: Nitric oxide (NO) is produced in different body organs in mammals and numerous physiological and pathological properties are attributed to this small molecule. The precursor of this substance in the body, L-arginine, is synthesized by the enzyme nitric oxide synthase (NOS), and it is catalyzed, and is inhibited by a substance called L-NG-nitroarginine methyl ester (L-NAME). In this study we investigated the qualitative and quantitative effects of nitric oxide on cerebellar histopathology in vivo environment via increasing and decreasing its production.
Methods: Forty Wister rats, weighing 200- 250 gr with a mean age of 8 weeks, were divided into 5 groups after making sure the rats were pregnant. Except the control group, the other pregnant groups, respectively received: 2 ml/kg normal saline, 200 mg/kg L-arginine, 20 mg/kg L-NAME and a mixture of the same doses of L-arginine and L-NAME on the third, fourth and fifth days of pregnancy. On day 18 of pregnancy, we anesthetized the rats, excised the cerebellum after craniotomy and fixed the organs in 10% formalin. We later prepared 5 to 6-micron in thickness tissue sections and dyed them by the routine Hematoxylin and eosin (HE) and Masson's Trichrom staining methods before studying them by light microscopy.
Results: There was a significant difference between the rats receiving L-arginine and the rats in other groups (P<0.01).
Conclusion: This study showed that L-NAME is capable of significantly decreasing the injury caused by nitric oxides in rat cerebellum.
