Sarraf Nejad A, Hoodei E, Siavoshi F, Maserrat S, Jadali Z, Shahrestani T,
Volume 59, Issue 4 (9 2001)
Abstract
Helicobacter pylori (H.pylori) is the most prominent causative agent of gastroduodenal diseases all over the world. Other manifestations such as urticaria and coronary heart diseases, also are suspected to be induced by H.pylori. Non invasive methods are preferred for diagnosis and ELISA, because of its reliability, speed, sensitivity and specificity is widely preferred as diagnostic tool. Previously we have used IFA, and here, we report an indirect ELISA technique for H.pylori diagnosis. First, 9 strains, of H.pylori isolated from biopsies, were cultured, and the soluble crude antigen was used to coat ELISA plates. Antigen concentration and conjugated antiserum dilution were optimised using checker board method. In this study the gold standard was: rapid urease test, culture and direct smear. Patient serum dillution and the cut-off value was determind, using 22 negative and 30 positive confirmed samples according to ROC curve and the results were compared with a commercial kit. The sensitivity and specificity of this method were 93.2 percent and 95.4 percent respectively. A commercial ELISA Kit, was used and compared simultaneously. The sensitivity and specificity were 87.8 percent and 73 percent respectively. Therefore, regarding the acceptable sensitivity and specificity, ease of work of ELISA, being economical and non-invasive, it can be employed in diagnosis of H.pylori infection and also in epidemiological studies.
Mehrnaz Mesdaghi, Mohammad Vodjgani, Eisa Salehi, Jamshid Hadjati, Abdolfattah Sarrafnejad, Masoud Movahedi, Farideh Berjisian, Tahereh Shahrestani,
Volume 68, Issue 1 (4 2010)
Abstract
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MicrosoftInternetExplorer4
Background: Allergic rhinitis is a common disorder with great morbidity. Its
prevalence has increased during recent years, therefore attracting attentions
to its mechanisms. Type 2 cytokines play a major role in allergies.
It has been proposed that Natural killer (NK) cells may
be able to produce type 2 cytokines. This study was done to
evaluate NK cells number and subtypes in patients
with allergic rhinitis, comparing healthy subjects.
Methods: In a case control study, patients with allergic rhinitis
were compared to healthy non-atopic subjects. Allergic rhinitis was diagnosed
according to ARIA guidelines. NK cells quantity was studied by staining of peripheral blood mono
nuclear cells with anti-CD16-FITC and anti-CD56-PE
and evaluated by two color flowcytometry. Intracellular cytokines were evaluated by tri-color flowcytometry.
NK cells were separated by magnetic beads,
and cultured for 72 hours. Secretion of IL-4, IL-5, IL-10, IL-13, and IFN-γ was measured by ELISA, in stimulated and unstimulated conditions.
Results: Patients had more CD16+ CD56+ NK cells than control group. IL-4+ NK cells were significantly higher in patients (p<0.001), but the number of IFN-γ+ NK cells was not different. Cytokine secretion of NK cells was similar in case and control groups. Although IL-13 level after stimulation seemed higher in patients, the difference
was not significant.
Conclusion: NK cells number is increased in patients with allergic rhinitis and a
considerable number of them produce IL-4.
