Showing 5 results for Shayegan
Tarabadi Fa, Shayegan M, Babaeie G, Talebian A,
Volume 60, Issue 1 (13 2002)
Abstract
CMV belongs to herpes viridea family and it is the largest human virus. Prevalence of CMV depends on age, race, geographic and socioeconomic factors. CMV infection has been a recognized complication of transfusion for about three decades, in an immature or compromised immune system situation. If a transplant candidate has not been infected with CMV and no CMV specific antibodies can be detected by serology, a primary infection could be transmitted via transfusion or transplantation. Patient who are under dialysis are susceptible to CMV infection, in addition of increase serum levels of p2-MG(Beta-2 Microglobulin).
Materials and Methods: we detected anti CMV (IgM -IgG) antibodies for 128 renal transplant candidates who were under intermittent heamodialysis and 1040 blood donors, as controls and compared serum p2-MG levels in 48 of the patients with 35 controls with ELISA technique. For 15 patients, R5 (Cuprophan) and for 7 patients S2 (Polysulphone) filters were used.
Results: Our data showed:
1-90 percent of normal blood donors and 89.8 percent of the patients were IgG positive. Statistical analysis showed no significant difference between these two groups (pO.Ol). This reflected high prevalence of CMV .
2- 0.2 percent of normal individuals and 2.3 percent of the patients were positive for IgM. There was significant difference in IgM between these two groups (p<0.01).
3- p2-MG levels in patient group were elevated and there was a significant differences between two groups (P <0.05 ) and no differences between common used dialysis filter in this study.
Iravani M, Shayegan M, Babaei Gh, Talebian A, Ghavamzadeh A, Babak Bahar, Aghaeipoor M,
Volume 62, Issue 3 (11 2004)
Abstract
Background: Graft-versus-host disease is one of the major complications after allogenic bone marrow transplantation, but it is not easy to anticipate the onset. Cytokines released by type 1 T-helper cells are thought to play a pivotal role in acute graft-versus-host disease (aGVHD). The ability to predict the likely occurrence of graft-versus-host-disease (GVHD) after BMT would be extremely valuable. By serially measuring serum levels of soluble IL-2 receptor (sIL-2R), IL-18 and following allogeneic bone marrow transplantation (BMT), we tried to define their relationship to aGVHD as complication of the transplantation and determine useful markers for aGVHD predictors.
Materials and Methods: Serum sIL-2R, IL-18, and levels were measured by sandwich ELISA in 219 sera samples from 39 patients (with hematological disorders before and after allogeneic BMT) and 28 controls. All patients received BMT from HLA-identical siblings.
Results: 25 patients developed aGVHD and serum levels of sIL-2 R and IL-18 , in sera drawn before transplantation , in patients with acute graft-versus-host disease (aGVHD +) , were increased in comparison of patients without acute graft-versus-host disease (aGVHD ¯) and control group and there wasn’t any significant differences in serum levels of sIL-2 R and IL-18 in aGVHD ¯ patients and controls. Serum level of IL-18, in aGVHD+ patients, was increased during day 3 - 24 after BMT, and there was a significant difference in patients with GVHD 0 – GVHD III. In majority of patients with acute GVHD (60 %) , the peak levels of IL-18 and IL-2R was achieved on day 10 after BMT and the rise in sIL-2R and IL-18 preceded of clinical signs of GVHD (mean day 15 after BMT). Level of IL-18 in patients with aGVHD had strongly correlated with the severity of aGVHD on Day 10 after BMT. IL-18 level mean (before BMT), in patients who received Busulfan and Fludarabin to treat aGVHD, was lower than in patients who received Busulfan - Endoxan, or Cyclophosphamide.
Conclusion: Our data concluded that IL-18 plays an important role in the development of aGVHD and IL-18 level might be an indicator for aGVHD, reflecting the severity of the disease. These findings suggest that IL-18 may play important roles in the pathogenesis of aGVHD and that measurement of serum IL-18 levels can be useful predictor of aGVHD.
M Shayegan, A.a Poor Fath , M Nomeiri, Gh Babaei, F.a Tarabadi,
Volume 62, Issue 4 (11 2004)
Abstract
Background: As increase of cytokines (like IL-1 , IL-6 , IL-8 and TNF- ) in platelet concentrates (PCs) during storage are involved in Febrile Non Hemolytic Transfusion Reactions (FNHTRs) after Platelet transfusion, the aim of this study was the survey of these cytokines level in PCs produced in Tehran Blood Center. Materials and Methods: This study proposed to determine if WBC reduction in PCs by pre-storage leuckodepletion filters reduced the levels of these cytokines during storage uo 3 days. Each of the PCs (n = 54) was prepared from single random donor (RD) by Platelet-Rich-Plasma (PRP), then were divided in four groups: 1- unfiltered non-irradiated RD-PCs (n= 13) 2- unfiltered and -irradiated RD-PCs (n=16) 3 - filtered non-irradiated RD-PCs (n=14) 4-filtered and -irradiated RD-PCs (n = 11). Cytokines levels in PCs supernatants were measured by ELISA kits according manufacture‘s instructions. Results: Our results showed : IL-8 was increased in unfiltered non-irradiated and IL-18 in -irradiated RD-PCs but not in the filtered RD-PCs in day 3 . Compared to unfiltered PCs in filtered units, pre-storage filtration prevented a rise in the IL-8 and TNF- in day 3 of storage .The concentration of IL-1 was lower than the minimal detectable concentration by the kits used for this purpose. IL-6 was detected only in 7 units of all filtered PCs in day 3. Conclusion: These data indicate that pre-storage leuko-reduction of PCs can prevent accumulation of IL-6, IL-8 and TNF- during storage.
Shayegan M, Poor Fathollah A.a, Namiri M, Babaei Gh,
Volume 63, Issue 4 (13 2005)
Abstract
Background: Tumor necrosis factor alpha (TNF-) is a major mediator of inflammatory responses and also plays a role in Febrile non-hemolytic reactions (FNHRs) after platelet concentrates (PCs) injection. It is thought contaminating WBCs are the source of these cytokine so inactivating or decreasing of these WBCs will be effective to decline of the cytokines. Due to difference results between different methods for TNF measurement, in this study we compared Immunoassay and Bioassay methods to determine TNF- in supernatants of PCs.
Materials and Methods: TNF- was measured by ELISA (Immunoassay) and by the L929 cytotoxicity Bioassay in supernatant of random donor PCs (RD-PC) prepared by platelet rich plasma (PRP). These platelet concentrates were divided in 4 groups: 1- unfiltered , non-irradiated RD-PCs (N=13) 2- unfiltered and -irradiated RD-PCs (N=16) 3- filtered , non-irradiated RD-PCs (N=14) 4- filtered and -irradiated RD-PCs (N=11)
Results: Our results showed: • TNF- was increased (by ELISA) in unfiltered non-irradiated units during storage but not in -irradiated and filtered RD-PCs in day 3. Compared to unfiltered PCs in filtered units, pre-storage filtration and irradiation prevented a rise in the TNF- in day 3 of storage. • There was no correlation between ELISA and the cytotoxicity L929 bioassay .
Conclusion: It has been previously reported that different quantitative results can be obtained when TNF alpha is measured in biological fluids by Bioassay and Immunoassay. This is thought to be related to the presence of antigenic forms of TNF alpha that cannot be detected by bioassay, such as complexes with soluble receptors (sTNF-R) or TNF alpha monomers.
Shahidi Sh, Seirafian Sh, Shayegan Nia B, Adilipoor H,
Volume 64, Issue 9 (1 2006)
Abstract
Background: Long term use of immunosuppressive therapy in transplant
recipients in order to prevent acute and chronic rejection increases the long
term risk of cancer. This study evaluates the incidence of different organs’
cancer after renal transplantation and immunosuppressive therapy.
Methods: This is a retrospective analysis of malignant tumors in renal graft
recipients with more than one year graft survival. Patients were assessed
according to their age, sex, diagnosis of cancer, immunosuppressive drugs,
donors and period of dialysis before transplantation.
Results: Evaluating all existing files in selected private clinics in Isfahan 350
patients were reviewed and 289 of them had entrance criteria. A total of 186
men and 103 women (mean age: 42.17±13.09 years) were included. They were
followed up over a mean period of 52.46±33.24 months. A total of six cases
(2.1%) of cancer were diagnosed in six recipients: All patients with cancer
were male with a mean age of 51.17±14.7 years (range: 26-68 years). Tumor
presented at a mean time of 51 months (rang: 15-82 months) after
transplantation. There were two patients with BCC, two patients with SCC and
two patients with lymphoma. Two patients died of progressive malignant
disease. Age, period of dialysis before transplantation, and using
immunosuppressive and anti-rejection drugs had no significant impact on
development of post transplant malignancy.
Conclusion: The frequency of tumors in these patients is lower than what
reported by other centers, probably due to short period of follow up and low
incidence of cancer in our general population. The risk of malignancy was 28
fold higher among transplant recipients than in general population. High risk
of cancer in this group, confirms the necessity of routine examination for
organ transplant recipients both before and after transplantation.
