Showing 3 results for Shokrzadeh
Shokrzadeh Sh, Saidijam M, Dehghan A, Esna-Ashari F, Farimani Sanoee M, Bahmanzadeh M, Alizadeh Z,
Volume 69, Issue 10 (5 2012)
Abstract
Normal
0
false
false
false
EN-US
X-NONE
AR-SA
MicrosoftInternetExplorer4
Background: The
techniques used in assisted reproductive technologies have progressed
considerably, but many embryos do not implant after transfer upon the use of
these techniques. One of the causes of infertility is repeated implantation
failure due to decreased endometrial receptivity. Furthermore, in clinical
conditions such as endometriosis and myoma, implantation decreases after embryo
transfer. In this case-control study the expression patterns of HOXA-10 and BTEB1 mRNAs were evaluated at the time
of implantation in patients with myoma and endometriosis.
Methods : In this study performed in Hamadan University of Medical Sciences
during 1389, the cases included 16 patients with endometriosis and myoma (8 in each group) and the
control group consisted of 8 fertile women. Endometrial sampling was performed at mid-secretory
phase. Later, the expression patterns of HOXA-10
and BTEB1
mRNAs were evaluated using a semi-quantitative RT-PCR method.
Results : The optimal PCR cycles determined were 30,
32 and 26 for HOXA10, BTEB1 and β-actin, respectively. Endometrial HOXA-10
and BTEB1 mRNA expression levels (normalized to ß-actin expression) at the time of implantation were significantly
decreased in the endometrium of infertile patients with endometriosis compared
with that of healthy fertile controls (P<0.05).
A similar pattern was seen in patients with
myomas for both HOXA10 and BTEB1 genes, (P<0.05).
Conclusion: It seems that lower
expression of HOXA-10 and BTEB1 mRNAs in the implantation window of endometrium that increase normally, could
account for some aspects of infertility in patients with endometriosis and
myoma.
Jeivad F, Abediankenari S, Shokrzadeh M, Ghasemi M, Taghvaei T, Ansari Z, Najafi Fard M, Hassannia H, Sayiari Mazandarani M, Biranvand E,
Volume 69, Issue 10 (5 2012)
Abstract
Normal
0
false
false
false
EN-US
X-NONE
AR-SA
MicrosoftInternetExplorer4
Background: Gastric cancer is one of the most common diseases of digestive
system with a low 5-year survival rate and metastasis is the main cause of death. Multi-factors,
such as changes in molecular pathways and deregulation of cells are involved in
the disease development. Epidermal growth factor receptor pathway (EGFR) which is associated with cell
proliferation and survival can influence cancer development. EGFR function is governed by its
genetic polymorphism thus, we aimed to study the tyrosine kinase domain gene
mutations of the receptor in patients with gastric cancer.
Methods : In this experimental study, 123 subjects (83 patients with gastric cancer and 40
normal subjects) were investigated in
north of Iran for EGFR gene polymorphisms during 1 year. Genomic DNA was extracted by DNA extraction kit according to the manufacture's protocol. Polymerase
chain reaction single-stranded conformation polymorphism (PCR-SSCP) and silver staining
were performed for investigating EGFR gene polymorphisms.
Results : The participants included 72 men and 44 women. Gene polymorphism in exon 18 was present in 10% of the study population but SSCP pattern in exon 19 did not show different migrate bands neither in patients nor in
normal subjects.
Conclusion: It seems that
screening for tyrosine kinas gene polymorphism of epidermal growth factor receptor
in patients with gastric cancer and use of tyrosine kinas inhibitors could be useful
in the prevention of disease progress and improvement of treatment process for
a better quality of life in these patients.
Saeid Abediankenari, Mohammad Shokrzadeh, Hamed Haghi Aminjan, Nafiseh Nasri, Ahad Alizadeh,
Volume 71, Issue 8 (November 2013)
Abstract
Background: Gastric cancer is the most prevalent cancer with poor survival in gastrointestinal tract. Caspase 3 and 9 play an important role in the development and progression of cancer. Polymorphisms in the genes for these enzymes can affect gene activity and thus may influence susceptibility to gastric cancer. In this study, caspase 3 and 9 genes polymorphisms in patients with gastric cancer were examined.
Methods: In a case - control study, 100 patients with gastric cancer and 100 healthy individuals were evaluated in the region rs4647601: G> T for caspase-3 and -1263 A> G gene promoter for caspase 9. DNA extraction was performed from whole blood according to manufacture protocol. RFLP-PCR method was carrying out for detection of caspase 3 and 9 genes genotype in two groups.
Results: In this study, 143 men and 57 women were evaluated. All of them were selected from the same race and geographical area. The results indicated an increase of the mutant G allele in the control group, which leads to a decreasing in the incidence of gastric cancer (P<0.0001, OR: 0.096, (%0.95CL) =0.04-0.23).
Conclusion: It seems that screening of -1263 A> caspase 9 polymorphism could be a useful marker in personal sensitivity to gastric cancer and help to cancer treatment and prevention process. It is concluded that caspase gene variation may be a diagnostic factor in the gastric cancer.
