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Showing 4 results for Takzaree
Male rat spermatogenesis influenced by Achillea millefolium L.
Takzaree N, Mortazavi H, Hassanzadeh G, Safaye S, Hossini M,
Volume 70, Issue 11 (3 2013)
Abstract

Background: Achillea millefolium or yarrow is a native plant in Europe and Iran. Yarrow has been used as a medicine historically, mainly because of its astringent effects. It is reported to be associated with the treatment of several ailments. Nowadays use of plants for medical purpose has become very common. Achillea millefolium L, Yarrow, is being used in traditional and modern medicine due to various chemical compounds. Considering the importance of birth control, finding a drug with less side effects inhibiting spermatogenesis seems to be necessary. The aim of the present study was to investigate the effect of ethanol extract of Achillea millefolium L. on spermato-genesis of male wistar rats.
Methods: In this study, 32 adult male wistar rats were used. The animals were divided to four groups of eight rats. The first group, received 200 mg/kg Achillea millefolium L. interaperitoneally, the second and third groups received 400, 800 mg/kg Achillea millefolium L. interaperitoneally, respectively. In the fourth group (control) distilled water was administered. After 20 days, the rats were sacrificed and testis tissues were histologically evaluated.
Results: Comparing to control group, in the experimental groups received the high doses of the extract, thickening of seminiferous tubules basement membrane, loss of germinal epithelium and testicular hyperemia were demonstrated (P<0.001).
Conclusion: Based on the results, high concentrations of Achillea millefolium L. leaded to structural and spermatogenesis changes in testis tissue.


Effects of L-NAME on morphometric parameters of stomach parietal cells in pregnant rats
Seyed Mohammad Hossein Noori Mugahi, Tahmineh Mokhtari , Ameneh Omidi , Nasrin Takzaree ,
Volume 72, Issue 2 (May 2014)
Abstract
Background: Considering nitric oxide (NO) has an important role in many biologic processes of cells and tissues such as in the digestive system and in this system act as a second messenger in pathological and physiological events in gastrointestinal region, in this study we investigated the effects of L-NG-Nitro arginine Methyl Ester (L-NAME) as the NO formation inhibitor on parietal cells of stomach in pregnant rats. Methods: Twenty four female rats were prepared and with eight weeks old and 200-250 g weight were used in this study. After matting of the female rats with the male rats, time of observing vaginal plaque considered as the zero day of pregnancy. Then the animals were divided into three groups of studying. Each group was containing eight rats. In this study, except the control group, the saline group received 2 ml/kg normal saline and experimental group received 20 ml/kg L-NAME interaperitoneally (IP), respectively on third, fourth, and fifth days of pregnancy for evaluation of its effects. On the 18th day of pregnancy, after anesthesia with ether, the animals were killed and dissected and the laparotomy was performed to separate the mother’s stomach. Then, the stomach was fixed in 10% formalin and after tissue passage, the sections were stained with Hematoxylin-Eosin (H&E). Then the changes of count and diameter in parietal cells were observed via light microscopy and Image Tools III. Results: Results of this study after analysis showed the significant changes in parietal cells count (mean 61.3±4.32) and its diameters (mean 16.12±1.18 µm) in L-NAME group in comparison to control and the sham groups in pregnant rats (P≤0.05). Conclusion: Results of this study showed L-NAME with effects on NO synthesis can reduce the count of parietal cells and increase its diameter in pregnant rats and has destructive effects on structure of stomach parietal cells in pregnancy rats.
Histological study of wound repair with topical aloe vera gel in rat
Nasrin Takzaree , Gholamreza Hassanzadeh , Mohammad Reza Rouini , Abbasali Keshtkar , Azadeh Manayi , Abbas Haajiakhondi ,
Volume 73, Issue 9 (December 2015)
Abstract

Background: One of the most important issues in medical sciences is wound healing and repair. Application of natural ingredients and herbs for treating ulcers has been in the history of human life. Nowadays, due to the lack of side effects of medicinal plants and a variety of effective compounds in plants, as well as numerous disadvantages of synthetic drugs there has been tendency to use medicinal plants in clinic. Aloe vera is an herbal drug used for treatment of dermal diseases. In this study we evaluated, effects of aloe vera on the wound healing through the microscopic techniques and cell counting.

Methods: In this experimental study, sixty Wistar rats weighing 200-250 g were placed under general anesthesia and sterile conditions. Then a square shape wound with 1.5´1.5 mm dimension was made on the back of the neck. Rats were randomly divided to control and experimental group’s. Each group was divided to three subgroups with 4, 7, and 14 study days. In 1st experimental group aloe vera was used twice on the wound surface and in 2nd experimental group was used once daily and the positive control group were applied phenytoin cream 1% from the zero days of surgery. The control group did not get any treatment on the wound surface. For histological studies, during the fourth, seventh and fourteenth day’s rats were sacrificed and samples were taken from the wound area and adjacent skin. After histological staining with hematoxylin and eosin (H&E) and Masson's trichrome stains, the cells were counted, wound surface and wound healing were investigated.

Results: The macroscopic and microscopic evaluation showed that wound healing increased because the fibroblast numbers in two experimental groups improved compared with control group. The percentage of wound healing on different days in the experimental and control groups were significant. Data were analyzed by using one-way ANOVA test and P< 0.05 was significant.

Conclusion: Present study showed that the twice application of topical aloe vera mucilage can result in rapid wound healing in rats.


Evaluation of Combination Treatment of BM-MSCs And Lotion containing honey, Aloe, propolis in the Healing of Second Degree Burns in Animal Models.
Tahere Abbasi Moayyer, Aziz Ghahhari,, Tayebeh Rastegar, Fateme Malek, Farzane Rezaei Yazdi, Kamyar Ghaffari Dafchahi, Nasrin Takzaree,
Volume 80, Issue 3 (June 2022)
Abstract
Background: One of the most important issues in medical science is the healing of burn wounds. The use of medicinal plants has been common for many years and today cell therapy offers new approaches to the management of skin wound healing. The present study aimed to evaluate the the combination treatment of lotion containing honey, Aloe, and propolis with BM-MSCs in the healing of second-degree burns in animal models.
Methods: this experimental study from October 2020 to November 2020, was performed in the animal house of Tehran University of Medical Sciences. 72 rats with an age range of 3-4 months and a weight of about 200-250 gr, after burns were randomly divided into 6 groups of 12 with study periods of 7, 14 and 21 days. The groups were as following: control (no treatment), Positive control (SSD1%), First experimental (Stem cells), Second experimental(lotion), Third experimental(Stem cells and lotion), and Fourth experimental (DMEM). To measure the percentage of healing from the wound surface on days 0, 4, 7, 10, 14, 17, and 21 photos were taken and the wound surface area was calculated by Image J software. After sacrificing rats, tissue samples were taken on days 7, 14 and 21 after burning induction. Samples were prepared for staining H&E and Trichrome Masson’s, as well as RT-PCR examination. The results were analyzed using Graph Pad Prism8 software and Tukey and one-way variance tests.
Results: Treatment preference was with the combination therapy group and then with the lotion group. According to macroscopic and microscopic images of H&E and Trichrome Mason, the highest amount of wound coverage and the presence of mature collagen fibers were also observed in the combination therapy group. The results of statistical analysis and scoring also showed increased epithelization, granulation tissue formation, collagen deposition, angiogenesis and fibroblast cell proliferation, and decreased inflammation in the combination therapy group. The combination therapy group also had the highest expression of the TGF-β gene. (P<0/05)
Conclusion: Due to its epithelialization, anti-inflammatory and angiogenesis properties, the lotion has healing properties in second-degree burn wounds and its simultaneous use with mesenchymal stem cells leads to healing and acceleration of burn wound healing.

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