Showing 11 results for Torabi
Sh Niroomanesh, Gh Behzadiannezhad, M Ebrahimi Torabi,
Volume 56, Issue 1 (30 1998)
Abstract
Between 270 patients complaining of symptoms of vaginitis, bacterial vaginosis was diagnosed in 68 women (%25) based on at least three of four criteria: 1. Homogenous vaginal discharge 2. Positive amine test 3. pH more than 4.5 4. Clue cell in wet smear. The results were compared to 55 normal controls.
Clue cell with more than %20 of epithelial cells was seen in %87 of cases and %5.5 of controls. A pH more than 4.5 showed the greatest sensitivity (%100) and the smallest specificity (%47). The most frequent complaints included increased discharge, bad odor, and sensing bad odor after coitus.
Mohammadi M, Mirjalili A, Habibi Gh, Falahi Sh, Sarafnejad A, Eatemadi A, Boutorabi Sm,
Volume 67, Issue 1 (4 2009)
Abstract
Background: Anti-dsDNA antibodies frequently found in the sera Systemic Lupus Erythematosus patients, particularly in active disease stage. Nowadays exploit different eukaryotic and prokaryotic dsDNA as antigen source and different reagents as binder. The aim of this study to compared two dsDNA different sources and tow different kinds of reagents for binder in ELISA test.
Methods: In this study bacterial genomic DNA from E.coli (ATCC 25922) and genomic DNA from calf thymus extracted with high purity and were used as antigens for IgG anti-dsDNA detection by ELISA. To coat dsDNA in microtiter wells, tow different kinds of reagents including methylated -BSA and poly-l-lysine (for pre-coating) are used. Sera from systemic lupus erythematosus patients and from normal blood donors are used to assess sensitivity and specificity of our ELISA test in compared with IF test and commercial kits.
Results: Our results displayed pre-coating of microtiter plates with methylated -BSA reduce nonspecific binding reaction and the relative sensitivity and specificity of ELISA increased when calf thymus DNA is employed as antigenic source in compared with IF test and commercial kits 80%, 88% and 100%, 98% respectively, but when E.coli DNA is used 73%, 69% and 85%, 79%, respectively.
Conclusion: The genomic DNA from calf thymus is a potentially useful source of antigen for detection of anti-dsDNA by ELISA. Also the use of methylatted- BSA could have an effective role in reducing of nonspecific binding reactions.
Nateghpour M M, Edrissian Gh, Torabi A, Raesi A, Motevalli-Haghi H, Abed-Khojasteh N, Ghobakhlo N,
Volume 67, Issue 3 (5 2009)
Abstract
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Background: Malaria is an
important parasitic vector-borne disease with considerable infectivity and
world-wide distribution. Since prevalence of chloroquine resistance in Plasmodium
falciparum at the malarious areas such as Iran
and reliable reports from many countries indicating emergence of chloroquine-
resistant strains of P.vivax, this study was conducted to monitor the
current response of vivax and falciparum plasmodia to chloroquine
in Bandar-Abbas district, a malarious area in Iran.
Methods: The study was
conducted at the Bandar-Abbas district in Hormozgan province, Iran. 123 patients were
enrolled and considered. The patients were treated with a standard 3-day regimen of
chloroquine and were followed-up clinically and parasitologically. The results
were interpreted as mean parasite clearance time (MPCT) in P. vivax and early
treatment failure (ETF), late treatment failure (LTF) and adequate clinical and parasitological response (ACPR) in P. falciparum.
Results: The patients with
vivax malaria were responded to the regimen of chloroquine within 24-216 hours. Most cases of
the parasite clearance time occurred at 48 hours (50.40%), and less of them at 120, 168, 192 and 216 hours with 0.81% for each of them. MPCT in this study was
calculated as 61.07 (±26/47) hours for all of the patients. 33.33% and 66.66% of the patients with falciparum malaria
were found at ACPR
and LTF groups, respectively.
Conclusion: This study confirms the efficacy of chloroquine on P.vivax.
The extended parasite clearance time in a number of patients may be an early
sign for reduced susceptibility of P.vivax to chloroquine in the studied
areas. Most of the patients with falciparum malaria (66.66%)
considered in this study did not respond to the regimen of chloroquine because
of chloroquine- resistance in P.falciparum at the area.
Masood Etemadifar, Mehrdokht Mazdeh, Hamid Reza Torabi, Majid Ghaffarpour, Mojtaba Azimian, Shiva Salami, Sayyed Mohammad Amir Shahkarami,
Volume 68, Issue 1 (4 2010)
Abstract
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Background: Recent studies present a
high prevalence of multiple sclerosis (MS) in Iran. Treatment with interferon is now the first choice in management
of MS. CinnoVexTM (an interferon beta 1-a) is available in
Iran, with achievement of the technology of producing beta interferon. The aim of
the present study is to evaluate the efficacy and safety of CinnoVexTM in a national study named CINA study.
Methods: This study was conducted from 2007 to 2008 in cities of Tehran, Isfahan, Mashhad,
Tabriz, and Shiraz. Patients with relapsing/remitting MS with 16-50 years of age and EDSS of <4 received CinnoVexTM (30µg/week, IM) after diagnosis by a neurologist. EDSS, drug side effects, and frequency of relapse were evaluated for one year
in four 3-month visits.
Results: A total of 1050 patients entered the study. Complete data
were collected from 627 (60%) patients. Mean age was 30.7±8.6 year and 514 (82%) were female. The most common onset presentations
were sensory symptoms (44%). Changes of EDSS through the study showed a significant decrease in the last 3-month of evaluation (p<0.05). Drug side effects
were observed in 47%, 50%, 61%, and 61.4% (p>0.05) and relapse was occurred in 13.4%, 15.7%, 16.9%, and 2.4% of the patients in the first, second, third,
and forth evaluation visits (p=0.001), respectively.
Conclusion: CinnoVexTM prevents progression and improves clinical course of MS. The conventional side
effects of beta interferon therapy, however, are observed with CinnoVexTM.
Somayeh Zamani, Fatemeh Fotouhi Chahouki, Zahra Nourmohammadi , Saeideh Sadeghi Neshat, Vahideh Mazaheri , Ali Torabi , Behrokh Farahmand ,
Volume 73, Issue 7 (October 2015)
Abstract
Background: The influenza virus is one of the most important factors for higher morbidity and mortality in the world. Recently, researchers have been focused on influenza conserved antigenic proteins such as hemagglutinin stalk domain (HA2) for vaccine production and serological studies. The HA2 plays a major role in the fusion of the virus with host cells membrane. The immunity system enables to produce antibody against HA2. The aim of this study is polyclonal antibody production against influenza HA2. Methods: This study was done in the Influenza Research Lab, Pasteur Institute of Iran, Tehran for one year from September 2013 to October 2014. In the present study, recombinant HA2 protein was produced in prokaryotic system and purified using Nickel affinity chromatography. The purified HA2 was mixed with Freund’s adjuvant (complete and incomplete) and injected into two New Zealand white rabbits by intramuscularly and subcutaneously routes. Immunization was continued for several months with two weeks interval. Before each immunization, blood was drawn by venous puncture from the rabbit ear. Function of rabbit's sera was evaluated using radial immunodiffusion (RID) in both forms, Single RID (SRID) and Double RID (DRID). Finally, antiserum activity against HA2 was evaluated using western blotting as serological assay. Results: Sedimentary line and zone was observed in RID assays (SRID and DRID) represent interaction between HA2 protein and anti- HA2 antibody. As well as, western blotting results was positive for HA2 protein. Therefore, these results showed that polyclonal antibody produced against HA2 protein can identify HA2 protein antigenic sites. Conclusion: These findings show that humoral immune responses have properly been stimulated in rabbits and these antibodies can identify HA2 protein and may be suitable for other serological methods.
Shahla Torabi, Mohammad Reza Asad, Arezoo Tabrizi,
Volume 74, Issue 6 (September 2016)
Abstract
Background: Diabetes is associated with many pathological changes and one of the most important consequences of the diabetes is hepatic injury. The present study was performed to investigate the effect of eight weeks endurance training with consumption of cinnamon supplementation on plasma concentrations of liver enzymes, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in women with type II diabetes.
Methods: In this quasi-experimental study, 36 female volunteers with type II diabetes (age 52.72±2.64 years and body mass index 29.28±2.94 Kg/m2) were participated. The subjects were homogenized regarding their body mass index and then were divided randomly into four groups (each group=9 patients): Training, training-cinnamon, cinnamon, and Control. Endurance training was performed for eight weeks (three sessions per week) at the intensity of 60-75% of maximum heart rate for 40-60 minutes. The consumption of cinnamon supplementation was 1.5 gr per day. Plasma concentrations of ALT and AST were measured following 12 hours fasting, 48 hours before and after performing the experiment, by the enzymatic method. Data were analyzed by paired t-test and factorial ANOVA, using SPSS version 21 (Chicago, IL, USA) and at the significant level of P<0.05.
Results: The levels of ALT was reduced in three experimental groups, which only its reduction was significant after consumption of cinnamon P<0.05. Also, plasma concentrations of AST increased in training-cinnamon and decreased in training and cinnamon groups which none of them was not significant. All interventions had no effect on blood fasting glucose in all experimental groups P>0.05. There was no significant difference between groups in pre and posttests.
Conclusion: The results confirm that cinnamon supplementation may be effective in improving the plasma levels of ALT but the intensity and duration of an effective exercise training especially with consumption of cinnamon supplementation simultaneously need more study in diabetic patients.
Farideh Zafari Zangeneh , Masoumeh Masoumi , Elahe Seyed Aboutorabi ,
Volume 76, Issue 9 (December 2018)
Abstract
Background: Genetic polymorphism is responsible for variations and individual differences. Polymorphism is a major factor of complex diseases with unknown etiology and cancer. Inconsistency in the symptoms of polycystic ovary syndrome (monthly disorder, hirsutism, obesity, diabetes, infertility) is one of the major pathological complications of this syndrome. The present study was conducted to evaluate the polymorphism gene β2 and β3 adrenergic receptors in women with polycystic ovary syndrome.
Methods: This cross-sectional study was performed on 200 patients with polycystic ovary syndrome (PCOS) in Imam Khomeini Hospital, Vali-e-Asr Infertile Clinic in Tehran, Iran, from March 2016 to April 2017. Blood samples in two groups (study and control) were obtained for genomics approved by the DNA Company based on the gene bank. Polymerase chain reaction (PCR) samples were extracted and then the primer design was performed by using Primer Express software, version 3.0 (Applied Biosystems, Foster City, CA, USA) and confirmed by using of the primer blast tool at the NCBI site in terms of compliance with the beta 3 adrenergic receptor gene. Analysis of protein changes was performed by using CLUSTALW (https://www.genome.jp/tools-bin/clustalw). Polymorphism was investigated on codons 16, 27, 113 and 164 from the beta2 adrenoceptor gene and codon 64 of the beta3 adrenergic receptor gene.
Results: The study of the codon beta2 of adrenoceptors showed that only codon 164 (Thr164Ile) polymorphism (44.4%) was significant (P<0/002) in study group. Homozygote and heterozygote ratios also show a significant difference between the study and control groups (P<0/004). Polymorphism exon 1 in codon 64 of beta3 adrenoceptor; which codes the amino acid tryptophan, indicates that the nucleotide T has changed to C. This finding confirms that mutagenic genotype can raise chance of getting to the polycystic ovary syndrome in women. OR: 2.546 (95% CI: 1.02-5.367) (P=0.012).
Conclusion: These results show that polymorphisms of codon 164 (Thr164Ile) of beta2 receptor gene and beta3 adrenergic receptor gene polymorphism Thr164Ile (rs 4994) associate with polycystic ovary syndrome and the risk of PCOS are significantly increased in mutation genotype women.
Minoo Movahedi, Maryam Sadat Torabipoor, Mahsa Soltan Mohammadi, Mamak Shariat, Fedyeh Haghollahi, Maryam Hajihashem,
Volume 79, Issue 5 (August 2021)
Abstract
Background: Normal vaginal delivery causes sexual dysfunction as well as urinary and fecal incontinence, which can threaten a person's quality of life. It is believed that pelvic floor muscle strength is the most important factor in improving sexual satisfaction and function and urinary control. Therefore, This study aimed to investigate the role of physiotherapy and pelvic floor exercises in reducing sexual dysfunction and incontinence in primiparous women.
Methods: This study was performed as a prospective randomized clinical trial on primiparous women who were referred to Al-Zahra and Shahid Beheshti educational hospitals in Isfahan between march 2019-Mars 2020. In this study, after obtaining ethics approval from the Medical Ethics Committee of Isfahan University of Medical Sciences, patients were divided into control (n=57) and intervention (n=57) groups. In the control group, no intervention was performed and only routine postpartum recommendations (multivitamin tablets and 500 mg calcium tablets once daily for two months) were prescribed. For the intervention group the pelvic floor physiotherapy was performed Sexual function and urinary and fecal incontinence were assessed in both groups at 8 and 16 weeks postpartum by completing two questionnaires.
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Results: In two groups of intervention and control; Mean maternal age, body mass index, and birth weight were not significant (P>0.05) In the intervention group, at the 8th and 16th weeks after delivery, the mean score of total sexual function, and the total score of female urinary and fecal incontinence was a significant difference. (P<0.05). Compared between the groups, the mean sexual function and pelvic floor irritation at 16 weeks postpartum were significantly different between the two groups. Physiotherapy and pelvic floor exercise intervention reduce pelvic floor irritation and improve sexual function.
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Conclusion: it seems that simultaneous performance of physiotherapy (biofeedback) and pelvic floor exercises by strengthening the strength of the pelvic floor muscles increases sexual function as well as a relative improvement of pelvic floor irritation in the postpartum period.
Ali Torabi, Behrokh Farahmand, Mohammadreza Zolfaghari , Fatemeh Fotouhi, Mohsen Zargar,
Volume 79, Issue 11 (February 2022)
Abstract
Background: Influenza vaccines based on conserved proteins are being developed persistently. The conserved protein vaccines based on Nucleoprotein (NP) are highly protected vaccines against influenza viruses that can be used as a Universal vaccine. Aluminum hydroxide (Alum) is the most common adjuvant used in vaccine formulation to improve immunization by altering the epitopes’ folds. However, due to its toxic effects on the nervous system, especially in infants and young children exposed to multiple vaccine injections during brain development, it is better to use more desirable options such as carbohydrate-based adjuvants. Sucrose ester (SE) is a carbohydrate and non-ionic surfactant that is compatible with the human body and environmentally friendly. This study evaluated the immunogenicity of recombinant NP molecule prepared in a prokaryotic with the accompaniment of sucrose ester adjuvant against lethal influenza virus challenge in a Balb/c mice model.
Methods: The recombinant vector of PET-28a-NP was used to produce NP molecule. The vaccines containing an NP with or without Alum or sucrose ester adjuvants were injected into the mice. The Effectiveness and immunogenicity were examined by evaluating the humeral immunity induction by Immunoglobulin G (IgG), and its subunits production, and cellular immunity induction by Interferon-Gamma (IFN-γ) and Interleukin-4 (IL-4) production by ELISA Method and also animal’s
surveillance was documented. The study took part at the Influenza and other respiratory viruses department of Pasteur institute of Iran in November 2018.
Results: The animals’ surveillance in the Np group was 57.1%, NP+SE was (71.4%), and NP+SE was 64.28%. Also, IgG and its subunits, IL4, and IFN-γ production in both Alum and SE combined vaccines compared to NP alone were significant.
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Conclusion: In combination with the carbohydrate adjuvant containing sucrose ester compared to the formulation with alum adjuvant, the NP could provide proper and considerable protection and immunity against the homologous strain (H1N1) of the Influenza A virus. It is recommended that SE usage as an adjuvant results in an adequate immune response and less toxic effect.
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Farkhondeh Jamshidi, Maryam Mirsalar, Mehdi Torabizadeh, Mehrnoosh Zakerkish , Maryam Rostami, Maryam Moradi ,
Volume 82, Issue 9 (December 2024)
Abstract
Background: Vitamin D deficiency is associated with various complications, including preterm birth, low birth weight, and neonatal infections. This study aimed to measure the level of vitamin D in the umbilical cord blood of full-term and premature babies and consider the prevalence of vitamin D deficiency in pregnant women.
Methods: In this case-control study, maternal vitamin D levels and umbilical cord blood vitamin D levels were estimated in March 2023 at Imam Khomeini Hospital in Ahvaz. The information obtained from questionnaires and laboratory measurements was statistically analyzed. Two groups of term or preterm infants delivered vaginally were enrolled in this study. As part of routine delivery room protocols, 2 mL of umbilical cord blood was collected from each neonate in a non-heparinized tube. The results were determined, and the prevalence of vitamin D deficiency was determined. The samples were categorized into three groups based on vitamin D serum levels: less than 20 ng/ml (severe deficiency), 20-29 ng/ml (moderate deficiency), and higher than 30 ng/ml (normal group).
Results: In this study, 39 term infants (55.7%) and 31 preterm infants (44.3%) participated. The mean umbilical cord blood vitamin D levels were 44.15 ± 24.33 ng/mL in term infants and 30.98 ± 17.05 ng/mL in preterm infants. Similarly, maternal vitamin D levels averaged 17.09 ± 7.87 ng/mL for term pregnancies and 18.76 ± 6.6 ng/mL for preterm pregnancies. Preterm babies had higher odds of underlying diseases (OR: 2.31, 95% CI: 0.51-10.53). Multivitamin consumption was more prevalent among mothers of premature babies (71%). No significant differences in vitamin D levels were found between term and preterm babies or their mothers (P > 0.05).
Conclusion: This study highlights the importance of multivitamin consumption among pregnant women, particularly those at risk of preterm delivery. The increased likelihood of underlying conditions and delayed recovery of vitamin D levels in preterm infants underscores the need for targeted interventions. Moreover, healthcare providers should emphasize vitamin D supplementation and monitoring.
Naseh Yousefi, Katayoun Moradi, Korosh Mansouri , Simin Sajadi , Masoud Torabi ,
Volume 82, Issue 12 (March 2025)
Abstract
Background: The most common cause of Shoulder pain is inflammation in the subacromial bursa. Intra-bursal corticosteroid injections are one of the most common methods in cases of bursitis. Recently, botulinum toxin A injection has also been used as a safe treatment in these patients. This injection does not have many of corticosteroid disadvantages. The most important disadvantages of this method are its cost. This study was performed to compare corticosteroids with botulinum toxin A in the treatment of subacromial bursitis by single injection.
Methods: This study is a prospective randomized clinical trial performed on patients with rotator cuff tendinopathy who were referred to the physical medicine and rehabilitation clinic of Iran University of Medical Sciences from November 2020 to October 2021. 54 patients with signs and symptoms of rotator cuff tendinopathy, were randomly assigned to one of the two groups of botulinum toxin A or corticosteroid injection. Routine treatment included exercise training for all patients. Patient's symptoms were assessed using VAS, Constant Score and SPADI criteria before treatment, two weeks and two months after injection.
Results: In the VAS study, both groups showed an improvement over baseline (P=0.000), which was significantly greater in the corticosteroid group. VAS between the first and second follow-up showed a decrease in the botulinum toxin A group and an increase in the corticosteroid group. In the Constant Score and SPADI study, both groups showed an improvement over baseline.
Conclusion: This study showed that a single-session injection of botulinum toxin A is less effective than corticosteroid in controlling symptoms of these patients. However, the therapeutic effect of botulinum toxin A, remains 2 months and unlike corticosteroid, has more reliability and durability.
