Tehran University Medical Journal

Background and Aim: Due to the increased usage of carbamazepine, phenytoin and Sodium Valproate, a higher number of intoxication is to be expected. The aim of this Study is demographic evaluation of this agents.

Materials and Methods: In this cross-sectional survey we studied 93 patients who were poisoned with these drugs from July 2003 until July 2004, in Loghman Hakeem hospital, Tehran, Iran.

Results: In this study we found these results: 36.6 % were male and 63.4 % of patients were female mean age of patients were about 24.5 y. twenty nine present (29) % were routinely on these drugs. Mean time between consumption and admission was 6 hours and 56% of them had decreased level of consciousness and 90% had high serum level of drugs. 80% of them showed complications such as respiratory distress, Urinary tract infection and etc and 4.5% of patients admitted in ICU and mortality rate was 2%

Conclusion: Results of this study are compatible with other studies and according to high prevalence of overdose with these drugs we must focus on prevention factors like education for suitable management, good care, psychiatric consult and proper Treatment.

Normal 0 false false false EN-GB X-NONE AR-SA MicrosoftInternetExplorer4 Background: Leishmaniasis is a parasitic infectious disease which causes skin sores. There is no effective laboratory screening tests for leishmaniasis. Some diagnostic techniques exist that allow parasite detection and species identification by special culture and microscopy, biochemical (Isoenzymes), immunologic (immunoassays), and molecular (PCR) approaches. Specific major objectives of this study was to genotyping of Leishmania species in Bam and Shiraz city.
Methods: A total of 83 samples of Leishmania were collected from patients clinically suspected of cutaneous leishmaniasis. The geographic distributions of the samples were 55 samples from Bam and 28 from Shiraz city. For this propose samples of skin and bloods were blotted on filter paper. Genomic DNA extracted with a Genomic DNA extraction kit (AccuPrep, BIONEER). Aliquots of extracted DNA were kept at -20°^C. region of ITS1 amplified with the published Leishmania-specific primers. 15-20mL of these amplicons, containing the amplified ITS1 region, was digested for 2h with HaeIII.
Results: All 55 samples from Bam were considered as L. tropica and the positive samples from Shiraz considered as L. tropica and just one sample was L. major which was belonged to a patient had previously traveled to Isfahan and Khuzestan.
Conclusion: In the current study a PCR technique was employed for amplification of Leishmania DNA directly in biological materials. Characterization of genus of Leishmania using RFLP-PCR method is too sensitive and too rapid, and there is no need for culturing the parasite for diagnosis.