Showing 4 results for Malassezia
Zareei M, Zibafar E, Daie Ghazvini R, Geramishoar M, Borjian Borujeni Z, Hossein Pour L, Hashemi Sj,
Volume 70, Issue 12 (3-2013)
Abstract
Background: The etiologic role of Malassezia furfur in onychomycosis, because of its controversial keratinolytic ability, has not been proven. The most reported cases are distal subungual onychomycosis (DSO). In our knowledge no cases of proximal onychomycosis (PO) has been reported. For the first time we report proximal onychomycosis. This case report describes the isolation of Malassezia furfur from fingernails.
Case presentation: An Iranian 56- year- old women had been referred to mycology lab with hyperkeratosis in proximal regions of right hand nails and clinical diagnosis of onychomycosis without paronychia in May 2012. She used several medicines for her cardiac disease, mental illness, severe stress and blood glucose fluctuation diseases. Scraping and sampling from nail lesions were done, budding yeast cells with broadband connections were observed in 15% KOH wet mounts. Also, other differentiation tests, consist of staining with methylen blue, cultures and biochemical tests were done. In order to rejecting the probable etiologic role of any dermatophytic or non-dermatophytic fungi in this case, samples were collected from other parts of the body by scotch tape and scraping with scalpel blade too, but the results of direct microscopy and culture were negative. Finally, Malassezia furfur was identified as the causative agent of onychomycosis.
Conclusion: Despite failure to prove Malassezia furfur keratinolytic ability, it can be the etiologic agent of proximal onychomycosis that shows the aggressive properties of this species. Its clinical importance is the easier transmission to hospitalized patients and other people.
Mahdi Zareei, Parivash Kordbacheh , Roshanak Daie Ghazvini , Ensieh Zibafar , Mohsen Geramishoar , Zeinab Borjian Borujeni , Mehdi Nazeri, Leila Hossein Pour, Mohammad Mirbulook Jalaly, Seyed Jamal Hashemi ,
Volume 71, Issue 5 (8-2013)
Abstract
Background: Malassezia Species are often commensal of the human skin and scalp that opportunistically in exist of particular predisposing factors, their proliferation increases as, in dandruff and seborrheic dermatitis which both togather affect more than 50% of humans, the excess proliferation of yeast in scalp, leads to scalp-flaking and causes physical and mental disorder in peaple, spacially in youth that their health and hiar hygiene and beauty is more important for them. Thus, this survey has been done for rapid, easy and inexpensive method to diagnosis of abnormal proliferation and invasive condition of Malassezia yeast and can be more benefical for proper treatment.
Methods: Sampling with scalpel scraping from scalp of volunteer persons that had not bathed at least two day ago were done and preparation of direct microscopic slides and staining with methylene blue were accomplished. Then, survey of morpholgic characte-ristics, yeast quantification and mycelium detection were done by direct microscopic examination.
Results: From 140 scalp samples of adult persons of both gender (male and female) with different age groups, observation of malassezia yeast in 93.5% (131) were positive and 6.5% (9) were negative in direct microscopic examination. Results of yeast quanti-fication in positive cases were: mild or normal flora 25.2%, intermediate 24.5%, severe 50.3%. Detection of mycelium in positive cases were 22.9% (30) (P=0.007 df=2).
Conclusion: Application of an accessible, easy and inexpensive method and a determi-nated pattern (yeast quantification with direct microscopic examination) to distinguish normal flora from abnormal condition (excess proliferation and mycelium production) in cases of Malassezia yeasts can be more useful to rapid diagnosis of abnormal pro-liferation and invasive condition in order to initiate a proper antifungal treatment.
Sanaz Tajiki , Roshanak Daie Ghazvini , Seyed Jamal Hashemi , Ensieh Zibafar , Mir Saeed Yekaninejad , Mahdi Zareei , Zeinab Borjian Boroujeni ,
Volume 75, Issue 9 (12-2017)
Abstract
Background: Seborrheic dermatitis (SD) is a chronic dermatitis with 1-3% prevalence and even with 33-83% in immunocompromised patients. Often, because of some of predisposing factors, abnormal proliferation of Malassezia yeasts (as a normal flora) is seen in patients that lead to observation of clinical symptoms in their bodies. The aim of this study was to evaluate of Malassezia yeasts colonization rate in patients with seborrheic dermatitis that has key importance to appropriate treatment.
Methods: This descriptive cross-sectional study was carried out during one-year period from January 2015 to February 2016 on 45 patients. According to the sample size formula, after visiting by a dermatologist and refer to the Medical Mycology Laboratory, Tehran University of Medical Sciences, patients were subjected to mycological examinations schedules. After recording the clinical symptoms and their specifications, sampling from all patients’ lesions was performed with sterile scalpel and a piece of scotch tape. Direct smears were prepared with 10% potassium hydroxide as a fungal clearing solution (KOH) and stained with methylene blue. The presence and rate of Malassezia yeast colonization was determined according to the standard procedures by direct microscopic examination.
Results: From a total of 45 under studied patients, 66.7% had moderate to severe colonization of Malassezia yeast. In existence of sweating and stress factors, severe colonization with significant differences was seen (P< 0.05). In 8 cases (17.7%), mycelium form of yeast was observed. The highest mycelium observation was seen in existence of stress with significant differences (P< 0.05).
Conclusion: In most of patients, yeast colonization rate was more than mild status, that emphasize on the etiological role of Malassezia yeasts at least as exacerbating factor in seborrheic dermatitis. On the other hand, the role of factors such as sweating and stress in inducing of severe colonization and invasive form of Malassezia yeasts in SD was confirmed, that should be considered to be treated as well as SD in therapeutic procedure.
Mahdi Zareei , Mohammad Shokri , Vida Mohegh, Reza Nedaei , Zeinab Borjian Boroujeni , Atefeh Ashenaei ,
Volume 76, Issue 6 (9-2018)
Abstract
Background: Tinea versicolor (TV) is common superficial fungal infection of the human skin characterized by scaling macula and mild disturbance of skin observed as pigmented and depigmented regions. Typically, it affects the chest, upper back, neck and shoulders. However, rarely, involvement of other unusual regions of the body such as the scalp and face, arms and legs, palms and soles, groin, vagina and axillary region has been reported.
Case presentation: This case report is a case of axillary TV caused by Malassezia furfur that involved a 32-year-old woman that referred to Ghaem Medical Mycology Laboratory, Department of Health, Rescue and Treatment of Iran Police Force, in Tehran at February of 2018. Clinical appearance of lesions was erythematous or brownish pigmented macula with mild scaling of skin in involved areas. After sampling, to diagnosis, direct smears of 15% Potassium hydroxide (KOH) with scales and stained once with methylene blue were prepared. In direct microscopic examination, budding yeast cells with typical scar and short curved mycelium were observed. To identifying, culture on Sabouraud dextrose agar and modified Dixon agar media and other differential tests were performed. Finally, Malassezia furfur was identified as a causative agent of disease. The patient was taken on treatment of ketoconazole ointment for 4 weeks, approximately and lesions were disappeared.
Conclusion: Malassezia furfur can causes Tinea versicolor in uncommon region such as axillary location. The clinicians must be aware of these variations in location of TV and perform the appropriate diagnostic workup when lesions have the characteristic morphology of TV despite an unusual location in order to differentiation from other disease such as candidiasis and erythrasma.
