K Pakshir , F Zini , E Aliakhooni ,
Volume 57, Issue 4 (7-1999)
Abstract
In the present study, we investigated total cell protein patterns of ten isolates of Epidermophyton Floccosum Var.pigmented by SDS-PAGE on 10% polyacrylamide resolving gels. Densitometric analysis of the gels allowed to detect more than 31 clearly detectable mycelial protein bands with molecular weights in the range of 12 to 98 KD proteins of 12.5, 13.5, 14.4, 16, 18.4, 19.7, 20.1, 23.5, 26, 27, 29, 30, 32.5, 34, 35.5, 37, 39.5, 40.5, 43, 47.5, 50, 63, 68, 75, 79, 82.5, 74.5, 90, 94, 96, 97-5 KD were present but their frequencies varied among the isolates. Protein bands of 18.4, 19.7, 20.1, 27, 29, 34, 37, 43, 47.5, 50, 63, 79, 94, KD were stronger and common among the isolates and could be specific to recognize genus differences. Protein analysis by SDS-PAGE could be considered an useful technique in identifying differences among the dermatophyte isolates.
