Saeed Eshraghi, Mohammad Mehdi Soltan Dalall, Fatemeh Fardsanei, Taghi Zahraii Salehi, Reza Ranjbar, Bahram Nikmanesh, Farzaneh Aminharati, Zahra Abdosamadi, Abolfazl Akbari,
Volume 67, Issue 12 (3-2010)
Abstract
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MicrosoftInternetExplorer4
Background: Salmonellosis is a bacterial gasteroenteritis
caused by different serovars of Salmonella. In
the recent years, Salmonella
enterica subspecies. Enterica
serovar enteritidis is a major cause of gastroenteritis and food poisoning in
the worldwide. Different
genus of salmonella is increasingly being resistant to common antibiotics. The
aim of this study was to determine the frequency and the antibiotic resistance
patterns of Salmonella enterica isolated from medical health centers in Tehran.
Methods: In this descriptive cross- sectional study from April
to December 2008, 1950
fecal specimens from children with diarrhea were cultivated to identify Salmonella
enteritidis. We
used Clinical and Laboratory Standard
Institute (CLSI) protocol
to determine resistance patern of the isolates to 16
different antibiotics.
Results: In this study, out of 26
isolates 14(54%) were S.
enteritidis, 2(8%) S.
para B, 6(23%)
S. para C,
3(11%) S. arizonea and 1(4%) S. para A.
all of them were sensitive to ceftazidime, cephalexin, cefotaxime, ceftiraxone,
ciprofloxacin, imipenem, meropenem, gentamicin and colistin sulfate. All
of the isolates were resistant to nitrofurantoin whereas 71.4%
of them were resistant to nalidixic acid.
Conclusions: The
most prevalent isolated salmonella was S.enteritidis. According
to high sensivity of these isolates to cephalosporin and flourqouinolon family,
they can used as infective treatment for salmonellosis infections.
Mohammad Mehdi Soltan Dallal , Mona Moshiri, Abbas Mirshafiey, Masoumeh Douraghi , Farhad Rezaie, Mehrdad Gholami ,
Volume 76, Issue 11 (2-2019)
Abstract
Background: Probiotics are living organisms that are beneficial for human health. Lactobacillus species has been considered as probiotic bacteria due to their adjustment of human immune responses and therapeutic effects in inflammatory disorders. The aim of the present study was to evaluate the effects of Lactobacillus probiotic strains on toll-like receptors (TLR2 and TLR4) expression in HT29 cell line (a human colon cancer cell line) infected with S. enteritidis.
Methods: This experimental study was done in Food Microbiology Research Center of Tehran University of Medical Sciences, Iran, from March 2016 to February 2017. In this study, two strains of Lactobacillus acidophilus PTCC 1643 and Lactobacillus casei PTCC 1608 were used. HT29 cells were cultured in RPMI medium containing fetal bovine serum and antibiotics. Then, the cells were treated with the Lactobacillus strains, after or before challenge with S. enteritidis. After total RNA extraction and cDNA synthesis, the capacity of probiotic lactobacilli to modulate TLR2 and TLR4 expression on treated and un-treated HT29 cells were assessed quantitatively using Real-time polymerase chain reaction technique with specific primers.
Results: Our findings indicated that after treatment of non-infected HT29 cells, with both the probiotics, the expression of TLR2 and TLR4 genes significantly increased. In contrast, the expression of these two genes in HT29 cells which were infected with S. Enteritidis was significantly reduced before and after treatment with each one of the probiotic bacteria. The anti-inflammatory effect of probiotic lactobacilli on S. enteritidis were confirmed in tests. This study showed that L. acidophilus and L. casei play a major role in boosting the innate immune responses, the TLR2 and TLR4 expression levels also decreased, pre and post-infection with S. enteritidis.
Conclusion: According to the results, both Lactobacillus strains have remarkable anti-inflammatory effect in pathogenicity of S. enteritidis, but L. acidophilus display greater anti-inflammatory activity than L. casei in this work. Additional in vivo and in vitro studies are required to further elucidate the mechanisms underlying this anti-inflammatory effect.
Kasra Mardani, Farhad Nikkhahi, Fatemeh Fardsanei,
Volume 81, Issue 9 (12-2023)
Abstract
Background: Salmonella enterica subspecies enterica serotype Enteritidis (S. Enteritidis) is one of the leading causes of food-borne infections associated with the consumption of contaminated food products of animal origin in humans. gastroenteritis due to Salmonella is usually a self-limiting disease and does not require antibiotic therapy. However, antibiotic treatment for salmonellosis may be lifesaving for patients with severe infections.The objective of the present study was to examine antimicrobial resistance and determine its genetic basis in recently isolated S.Enteritidis strains.
Methods: During this study, in a cross-sectional descriptive study, 44 isolates of Salmonella enteritidis from human sources were investigated between September 2021 and September 2022.. After identification of the isolates using phenotypic and molecular methods by Multoplex-PCR, antibiotic resistance testing was performed according CLSI 2023. The strains were examined for the presence of qnrA,qnrB,qnrS and gyrA resistance genes by PCR.
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Results: In a cross-sectional descriptive study, 44 isolates of Salmonella Enteritidis from human sources were investigated between September 2022 and September 2023. 100% of the strains were sensitive to imipenem and meropenem, and the sensitivity to the antibiotics ceftriaxone, ceftazidime, and cefotaxime were 93.2%, 90.9%, and 94.1%, respectively. 81.8% of isolates were sensitive to cotrimoxazole, sensitivity to ampicillin was 84.1%. Only 9.1% of isolates were sensitive to ciprofloxacin. Based on MIC results, 16 isolates had MIC between 0.002 and 0.064 and were placed in the sensitive area. 28 isolates had MIC between 0.125 and 0.5 and were placed in the area of reduced sensitivity. None of the strains resistant to disk diffusion method were resistant to MIC method. qnrA, qnrB and qnrS genes were not observed among ciprofloxacin resistant strains. All nalidixic acid resistant strains had gyrA gene.
Conclusion: In general, it was shown in this study that the resistance to the fluoroquinolone family is increasing among Salmonella Enteritidis isolates. On the other hand, we see a decrease in the sensitivity and prevalence of strains resistant to broad-spectrum cephalosporins among serovar Enteritidis, which is the drug of choice for extraintestinal infections.
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