Showing 5 results for Th1
Ahmadinejad Z, Mobaen A.r, Kariminia A, Afhami Sh, Hatmi Zn, Torkabadi E, Yalda A,
Volume 65, Issue 2 (3-2008)
Abstract
Background: Sepsis is the leading cause of death in critically ill patients throughout the world. The incidence is increasing despite the major advances in the development of antimicrobial agents and other supportive treatments. Based on multiple studies, it has been shown that patient outcome depends on Th1 and Th2 cytokine response. Moreover, whenever the Th2 response is predominant, the sepsis is more severe. The aim of this study was to evaluate the correlation between cytokine levels and the severity of sepsis in patients.
Methods: A cross-sectional study on the cellular levels of several pro-inflammatory cytokines was carried out in patients with sepsis and severe sepsis. The study included 37 patients (24 men and 13 women), 26 of them had sepsis and 11 had the severe form of sepsis Thirty-seven healthy volunteers served as controls. The average age of the patients was 57 years (±23.3 years), with a range of 21 to 92 years. From the whole blood of the subjects, we separated the monocytes and leukocytes, which were then cultured. Using an ELISA method, we measured levels of IFN- and IL-12 (associated with Th1), and IL-4 and IL-10 (associated with Th2) in the cultured cells with and without cell stimulation.
Results: No correlation was found for IFN- production in the cells of patients with sepsis and severe sepsis, regardless of whether the patients had died or survived. However, IL-12 levels were significantly decreased in severe sepsis compared with those of sepsis patients (P=0.048). Furthermore, the cells of expired patients also had significantly decreased IL-12 levels compared with those of surviving patients (P=0.028). We also found that the levels of IFN-, IL-4, and IL-10 were decreased in patients compared with those of controls, which correlated to their production. However, there was no correlation for IL-12 production between the cells of the patients compared with those of the controls. There was also no correlation for cytokine production between men and women with sepsis and in adults compared with that of elderly patients (>55 years old).
Conclusion: We have shown that the predominating T helper cell subset in patients with severe sepsis, as well as expired patients, is Th2. In conclusion, the correlation of Th1 cytokine production and progression of sepsis was demonstrated. Most probably IL-12 levels would be significantly lower in patients with severe sepsis and those who expired.
Mosleh A, Darbooy Sh, Khoshnevis Ansari Sh, Mohammadi M,
Volume 65, Issue 14 (3-2008)
Abstract
Background: Rationalize of drug use in societies is one of the main responsibilities of
health policy makers. In our country irrational use of dugs has increased in the recent
years, for example one study in 1998 has shown that average number of medicines per
prescription was 3.6, percentage of prescriptions containing antibiotics was 43% and
percentage of prescriptions containing Injections was 39%. One of the best tools for
evaluation of drug use is the WHO guideline for calculating prescribing indicators. In this
study, we had an assessment about prescribing patterns in South of Tehran, Islamshahr
and Rey Health Centers.
Methods: In order to evaluating prescribing indicators in Tehran University of Medical
Sciences region 35 facilities which had pharmacy were selected according to WHO
gridline and 4190 prescription from these facilities were studied. Indicators were
calculated according to formulas has explained in article.
Results: The average number of drug per prescription was 2.58, percentage of drug
prescribed by generic name: 99.8%, percentage of encounters prescribed Antibiotics:
62.39% percentage of encounters prescribed Injection: 28.96% & the percentage of drugs
prescribed from PHC formulary 99.46%. These findings were almost similar in the three
Health Centers.
Conclusions: Health facilities are one of the most important bases to improve rational
use of Drugs and general practitioners are the major chain in RUD cycle. Results show
that we need to design intervention especially educational interventions to improve two
WHO prescribing indicators, percentage of encounters prescribed Antibiotics &
Injections in this region. For reaching this goals we need to design educational programs
for physicians, pharmacists and people too. These educations can be as workshops,
seminars, conferences or printed materials such as books, leaflets and etc
Farhadi M, Tabatabaee A, Shekarabi M, Noorbaksh S, Khatib M, Javadinia Sh,
Volume 69, Issue 9 (12-2011)
Abstract
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Background: Too many studies are in the process
of determining the probable role of immune system in the etiopathogenesis of
nasal polyposis. This study was designed to identify the probable participation
of Th1, Th2 lymphocytes in the induction and progression
of nasal polyposis.
Methods: Seventy-five patients, 42 male and 33 female, with
nasal polyposis were examined for total serum IgE, specific serum IgE and reaction to skin test for differentiating allergic from
non-allergic participants in Rasoul Akram Hospital during 2010. To determine the possible correlation of allergic
reactions in the upper respiratory tract and nasal polyposis, cytokine gene
expression was evaluated on the extracted RNA by
RT-PCR. The data were analyzed by using c2, independent t-test, correlation and
Receiver operating characteristic (ROC)
curve.
Results: The mean
age of participants was 38 years (18-81 years). IFN-γ
and IL-4 gene expressions were more prevalent
in allergic than non-allergic individuals (IFN-γ:
39.5% vs. 14.2%, P=0.3 and IL-4: 44.7% vs. 18.9%, P=0.02, respectively). IL-10 and IL-12 (P35 and P40 fractions) genes were not
significantly different between the two groups. IL-10 and IL-12 (P35, P40) genes did not differ significantly either.
Conclusion: This research suggests that overproduction of
cytokines and an imbalance of Th1
and Th2 cell production may
play an important role in the pathophysiology of allergic or non-allergic nasal
polyp formation. Thus, although nasal polyposis is a multifactorial disease
with several different etiological factors, chronic persistent inflammation is
undoubtedly a major factor irrespective of the etiology.
Etaati Z, Moazzami Godarzi R, Kalhori F, Sobhani Sa, Solati M, Alavi A, Tashnizi Sh, Naderi N,
Volume 70, Issue 1 (4-2012)
Abstract
Background: Diabetes mellitus (DM) is a group of metabolic disorders such as DM I, DM II, secondary causes of DM and gestational diabetes mellitus characterized by hyperglycemic phonotype. The etiology of gestational diabetes mellitus is unknown. Recent studies address the chronic activity of immune system against infections (not autoimmunity) as an important cause of gestational diabetes mellitus. This study aimed to compare T-helper cells 1 and 2 cytokines and associated antibodies in patients with gestational diabetes mellitus and normal pregnant women.
Methods: This cross-sectional study was performed on 45 female patients with GDM and 45 healthy pregnant women in Bandar Abbas, Iran, from 2008- 2009. The exclusion criteria were presence of any infectious diseases or autoimmune disorders such as SLE or RA. Present and past medical histories were taken from the participants thorough physical examination. Blood samples (10 mL) were drawn and sent to laboratory for measuring serum IgE, IgG1, IgG2, IgG3, IgG4, interleukin-10 (IL-10), interleukin-12 (IL-12), transforming growth factor-beta (TGF1), and interferon-gamma (IFN) measurements. T-test and Kolmogorov-Smirnov test were used for data analysis.
Results: The mean age of the patients with GDM and healthy pregnant women was 32.5 and 27.9 yrs, respectively. T-helper 1 and 2 associated antibodies and cytokines had no significant differences between the case and control groups.
Conclusion: The changes in T-helper 1 and 2 associated antibodies and cytokines are not associated with gestational diabetes mellitus and could not be considered as a predictor for gestational diabetes mellitus.
Reza Yazdani , Mazdak Ganjalikhani Hakemi, Roya Sherkat , Abbas Rezaei , Rahim Farahani , Behrouz Beiranvand ,
Volume 72, Issue 9 (12-2014)
Abstract
Background: Asthma as an airway disease is identified by increase network respon-siveness of the trachea and bronchus to a specific stimulus. Th17 cells through produc-tion of IL-17 have important role in inflammation and autoimmune diseases .In some studies has been shown which IL-17 as major cytokine of Th17 probably has im-portant role in the pathogenesis of allergy and asthma.
Methods: Total mRNA extracted from whole blood samples and sputum of 23 asthma patients and 23 normal controls. Then, total RNA was converted into cDNA according to the manufacturer’s instructions. Finally, the transcript levels of IL-17 were quanti-fied by the real-time quantitative PCR. Twenty-three patients with asthma were diag-nosed and selected according to the global initiative for asthma (GINA) and none of the patients had taken the medication at least three week before sampling. Healthy in-dividuals did not have any history of allergy, asthma and other inflammatory diseases at the time of sampling. All of experiments have done in Isfahan University of Medical Sciences, Iran during May to February, 2013.
Results: This study showed a significant increase in transcript levels of IL-17 in the blood (287±79 versus 1/18±0/13) and sputum samples of the patients (64±28 versus 0/9±0/1) in comparison with normal individuals (P= 0.000, P= 0.029 respectively). It al-so revealed that the expression levels of the cytokines in the serum samples of the asthmatics were significantly more than their levels in patient’s sputum samples (P= 0.000). However, there was no significant difference between the cytokines expression levels in serum samples and sputum samples of the controls (P> 0.05).
Conclusion: In this study, we showed which the expression of IL-17 was increased in serum and sputum of asthmatic patients compared to healthy controls, this could re-sult in elevation of neutrophils population and activation of pulmonary neutrophil.
