Mehrnaz Mesdaghi, Mohammad Vodjgani, Eisa Salehi, Jamshid Hadjati, Abdolfattah Sarrafnejad, Masoud Movahedi, Farideh Berjisian, Tahereh Shahrestani,
Volume 68, Issue 1 (4-2010)
Abstract
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Background: Allergic rhinitis is a common disorder with great morbidity. Its
prevalence has increased during recent years, therefore attracting attentions
to its mechanisms. Type 2 cytokines play a major role in allergies.
It has been proposed that Natural killer (NK) cells may
be able to produce type 2 cytokines. This study was done to
evaluate NK cells number and subtypes in patients
with allergic rhinitis, comparing healthy subjects.
Methods: In a case control study, patients with allergic rhinitis
were compared to healthy non-atopic subjects. Allergic rhinitis was diagnosed
according to ARIA guidelines. NK cells quantity was studied by staining of peripheral blood mono
nuclear cells with anti-CD16-FITC and anti-CD56-PE
and evaluated by two color flowcytometry. Intracellular cytokines were evaluated by tri-color flowcytometry.
NK cells were separated by magnetic beads,
and cultured for 72 hours. Secretion of IL-4, IL-5, IL-10, IL-13, and IFN-γ was measured by ELISA, in stimulated and unstimulated conditions.
Results: Patients had more CD16+ CD56+ NK cells than control group. IL-4+ NK cells were significantly higher in patients (p<0.001), but the number of IFN-γ+ NK cells was not different. Cytokine secretion of NK cells was similar in case and control groups. Although IL-13 level after stimulation seemed higher in patients, the difference
was not significant.
Conclusion: NK cells number is increased in patients with allergic rhinitis and a
considerable number of them produce IL-4.
Rasool Molatefi, Adel Ahadi, Hossein Salehzadeh, Elham Safarzadeh, Hadi Abbasi,
Volume 78, Issue 8 (11-2020)
Abstract
Background: Because of geographic variation and regional types of allergens, it is recommended that allergists identify common allergens in their area of activity. In the present study, the prevalence of respiratory allergens in patients with asthma and allergic rhinitis was investigated.
Methods: This study is descriptive and retrospective. The required data of this study were extracted from the records of 661 patients who were referred to the Asthma and Allergy Clinic of Ardabil University of Medical Sciences (Ardabil province, Northwest of Iran) from April 2016 to March 2017. After confirmation of allergic asthma or rhinitis by the asthma and allergy specialist, these patients were subjected to a prick test with allergen extracts. In the group of grasses the extract of nine grasses, in trees the extract of 11 Tree mix, in weeds the extract of Common weed mix, in animal allergens the three extracts of Standard cat, Dog epithelia, Cockroach mix, in weeds the Mite mix and in fungi, three extracts of Mold mix1, Alternaria and Aspergillus mix were used. All the data were analyzed using SPSS software, version 25.
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Results: According to results, of the 661 patients who were studied, 462 ones had positive and 273 had significant prick test results. The grasses group with 293 positive (44.3%) and weeds group with 259 positive results (39.2%) were more common than the other categories. Among the significant positive tests, these two categories with 161 and 142 significant positive tests were indicated as the most common allergens. Respiratory allergens were also more common in patients with a family history of allergies in weeds and trees categories, but there was no significant difference in sensitization pattern according to the history of tonsillectomy.
Conclusion: Patients in Ardabil with allergic rhinitis and asthma were more sensitized to weeds and grass mixtures. It is recommended to provide preventive recommendations to allergic patients and the development of localized skin testing packages and additional studies should be conducted focusing on these two categories of allergens.
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