Showing 5 results for Colonization
Gh Khataie , N Shahrokhi ,
Volume 56, Issue 6 (7-1998)
Abstract
Group B streptococcus (GBS) is the most important pathogen identified in bacterial cultures in neonatal sepsis, sepecially with early-onset in developed countries (approximately 1-5/1000 deliveries). Neonatal colonization with group B streptococcus results primarily from vertical transmission during the birth process. GBS carrier rate in pregnant women varies from 4.6 to 41 percent in different geographic populations. Contamination of neonates during passage through the birth canal is high (more than 50%). Of the 191 pregnant women screened in this study, 28 (14.7%) were found to be colonized with GBS, by the culture method. Direct CIE and SCA tests on SBM (Selective Broth Medium) containing mixed flora showed that only 11.5% and 18.3% had positive reaction. A total of 530 patients were studied. GBS was isolated from the blood of 4 infants (5.5%, 4 vs 73 positive cultures). Of 181 cultures of CSF only one case was positive for GBS (8.3%) and had meningitis. In another part of experiment, two false positive reactions were found using serum specimen for detection of GBS antigen by CIE. Sensitivity of CIE and SCA both were 75%, specificity, 99.3% and 98.7%. Conclusion: Although specimen collection and microbiologic methods are important factors in identification of women colonized with GBS, there is significant variation in the proportion of women colonization with GBS. This study suggests that GBS is a much less important cause of neonatal sepsis, but further studies are needed to explore these important issues.
Hasibi M, Iravani Bm,
Volume 65, Issue 3 (6-2007)
Abstract
Background: Staphylococcus aureus is one of the most common causes of nosocomial infections with high morbidity and mortality rate. Traditionally, methicillin resistant staphylococcus aureus has been considered a major nosocomial pathogen in healthcare facilities, but in the past decade, it has been observed emerging in the community as well. Informations regarding hospital microbial colonization could be an important step for prevention of nosocomial infections. Our objective was clarifying the prevalence of methicillin resistant and vancomycin resistant staphylococcus aureus colonization in nasopharynx.
Methods: A descriptive cross sectional study was carried on 106 patients and nursing staff of surgery and hemodialysis wards in Amir-Alam hospital from April 2005 to July 2005. The samples were collected from nasal region of cases using cotton swab by two experienced technician and were sent to laboratory for culture and antibiogram.
Results: Twenty six (29.5%) out of 106 cases were nasopharyngeal carriers of staphylococcus aureus. Eight cases (7.5%) had methicillin resistant staphylococcus aureus. The most frequent colonization rate was seen in hemodialysis nursing staff and in all of them methicillin resistant staphylococcus aureus was reported. Carrier rates in hemodialysis patients were twice compared to surgery ward patients. The interesting point was that no sample of vancomycin resistant staphylococcus aureus was isolated.
Conclusion: Prevalence of methicillin resistant staphylococcus aureus colonization seems to be increased therefore proper management for controlling this problem is mandatory. The results of the present study suggest that the prevalence of methicillin resistant staphylococcus aureus infections is higher than was expected in Iran and vigorous preventive strategies should therefore be taken to stop the growth of this major health problem.
Khosravi N, Noorbakhsh S, Tabatabaei A, Ghavami Y,
Volume 70, Issue 11 (2-2013)
Abstract
Background: Infection with group B streptococcus (GBS) can present with respiratory distress, Pneumonia, meningitis and Osteomyelitis in neonates. The aim of this study was to determine the prevalence of GBS colonization in trachea of intubated neonates.
Methods: This observational analytic study was performed upon 33 intubated neonates due to respiratory distress in neonatal intensive care unit (NICU) Rasoul Hospital in Tehran, Iran during 2010-2012. Tracheal secretions cultured upon TODD-HEWITT BROTH and sheep blood agar 5%., chi-square test was used for compare the qualitative variables. P<0.05 was considered meaningful.
Results: Three cases had positive streptococcal culture (9.1%) and four cases had posi-tive culture for non-streptococcal organisms. no meaningful relation observed between positive GBS culture and neonatal gender, kind of delivery, PROM.
Conclusion: Prevalence of GBS positive results (9%) in present study is very close to GBS colonization in pregnant women although the higher colonization rate of pregnant women are expected.
Narges Sadat Taherzadeh , Farideh Zaini , Roshanak Daie Ghazvini , Sasan Rezaie , Mahmoud Mahmoudi , Maliheh Kadivar , Fatemeh Sadat Nayeri , Mahin Safara , Parivash Kordbacheh ,
Volume 73, Issue 11 (2-2016)
Abstract
Background: Over the last two decades invasive candidiasis has become an increasing problem in neonatal intensive care units (NICUs). Colonization of skin and mucous membranes with Candida spp. is important factor in the pathogenesis of neonatal infection and several colonized sites are major risk factors evoking higher frequencies of progression to invasive candidiasis. The aim of this study was to detect Candida colonization in NICU patients.
Methods: This cross-sectional study was conducted on 93 neonates in NICUs at Imam Khomeini and Children Medical Center Hospitals in Tehran. Cutaneous and mucous membrane samples obtained at first, third, and seventh days of patients’ stay in NICUs during nine months from August 2013 to May 2014. The samples were primarily cultured on CHROMagar Candida medium. The cultured media were incubated at 35°C for 48h and evaluated based on colony color produced on CHROMagar Candida. In addition, isolated colonies were cultured on Corn Meal Agar medium supplemented with tween 80 for identification of Candida spp. based on their morphology. Finally, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was performed for definite identification of isolated species.
Results: Colonization by Candida spp. was occurred in 20.43% of neonates. Fifteen and four patients colonized with one and two different Candida spp., respectively. Isolated Candida spp. identified as; C. parapsilosis (n: 10), C. albicans (n: 7), C. tropicalis (n: 3), C. guilliermondii (n: 2), and C. krusei (n: 1). In present study non-albicans Candia species were dominant (69.56%) and C. parapsilosis was the most frequent isolate (43.47%). Using Fisher's exact test, the correlation between fungal colonization with low birth weight, low gestational age, and duration of hospital stay was found to be statistically significant (P=0.003).
Conclusion: The results of this study imply to the candida species colonization of neonates. Neonates in NICU are at the highest risk for severe infection with Candida parapsilosis. Therefore, isolation of C. parapsilosis as the most common species (43.47%) in present study was noteworthy.
Sanaz Tajiki , Roshanak Daie Ghazvini , Seyed Jamal Hashemi , Ensieh Zibafar , Mir Saeed Yekaninejad , Mahdi Zareei , Zeinab Borjian Boroujeni ,
Volume 75, Issue 9 (12-2017)
Abstract
Background: Seborrheic dermatitis (SD) is a chronic dermatitis with 1-3% prevalence and even with 33-83% in immunocompromised patients. Often, because of some of predisposing factors, abnormal proliferation of Malassezia yeasts (as a normal flora) is seen in patients that lead to observation of clinical symptoms in their bodies. The aim of this study was to evaluate of Malassezia yeasts colonization rate in patients with seborrheic dermatitis that has key importance to appropriate treatment.
Methods: This descriptive cross-sectional study was carried out during one-year period from January 2015 to February 2016 on 45 patients. According to the sample size formula, after visiting by a dermatologist and refer to the Medical Mycology Laboratory, Tehran University of Medical Sciences, patients were subjected to mycological examinations schedules. After recording the clinical symptoms and their specifications, sampling from all patients’ lesions was performed with sterile scalpel and a piece of scotch tape. Direct smears were prepared with 10% potassium hydroxide as a fungal clearing solution (KOH) and stained with methylene blue. The presence and rate of Malassezia yeast colonization was determined according to the standard procedures by direct microscopic examination.
Results: From a total of 45 under studied patients, 66.7% had moderate to severe colonization of Malassezia yeast. In existence of sweating and stress factors, severe colonization with significant differences was seen (P< 0.05). In 8 cases (17.7%), mycelium form of yeast was observed. The highest mycelium observation was seen in existence of stress with significant differences (P< 0.05).
Conclusion: In most of patients, yeast colonization rate was more than mild status, that emphasize on the etiological role of Malassezia yeasts at least as exacerbating factor in seborrheic dermatitis. On the other hand, the role of factors such as sweating and stress in inducing of severe colonization and invasive form of Malassezia yeasts in SD was confirmed, that should be considered to be treated as well as SD in therapeutic procedure.
