Showing 8 results for Extract
Taghizadeh-Jahed M, Jarolmasjed Sh, Mohamadnejad S, Rezaii A, Delazar A,
Volume 66, Issue 9 (12-2008)
Abstract
Background: Because of eventual side effects of chemical drugs, the efficacy of natural wound healing accelerators in long-term diseases and some situations is demanded to practitioners. The initial aim of our study was to assess full thickness excisional skin wound healing and inflammation diminution, Morphometrically and Histopathologically, after topical application of dried extract of Echinacea purpurea aerial part in rats, compared with zinc oxide.
Methods: Sixty wistar rats received four full thickness excisional wounds with the aim of surgical punch on the back skin under surgical anesthesia. All rats were randomly divided into groups 1, 2 and 3, of Echinacea purpurea, zinc oxide and control, respectively. All of them were treated topically once a day for 21 uninterrupted days. Healing of the wounds was daily measured by taking digital photographs and analysis. Histopathologic assessment was carried out in the 0th, 3rd, 7th, 14th, and 21st days of treatment period as well, and wound healing was assessed using 1 to 6 healing grades.
Results: According to Morphometric findings, the wound contraction rate in group 1 after 21 days of skin punching, with wound size of 0.18±0.03 mm2 in contrast with group 2, 2.81±0.21mm2, was much higher than that in other groups. Group 1 with wound contraction rate of 2.5 times in the day 7 and 3 times in the day 14 more than group 2, had the best wound contraction (p<0.01). histopathologic assessment revealed that, overall healing rate in the group 1 was highest (p<0.01).
Conclusion: Echinacea purpurea dried herbal extract could be a new capable remedy to accelerate skin wound healing because of its potential anti-phlogosis and wound healing stimulatory properties.
Kabiri F, Nejati V, Tukmechi A, Delirezh N, Nikbakhsh P,
Volume 68, Issue 12 (3-2011)
Abstract
Background: Lactobacillus species are genetically diverse groups of Lactic Acid
Bacteria (LAB) that have been
introduced as probiotics, because of some characteristics such as their anti-tumor properties, helping the intestinal flora balance, production of
antibiotics, stimulation of host immune response, etc. The aim of this study was to investigate the effects of cytoplasmic extraction and cell wall of
Lactobacillus species isolated from the intestine of common carp on human chronic myelocytic leukemia or K562 cancer cell lines.
Methods: The intestinal contents of 115 common carp captured from the natural resources of West Azerbaijan province in Iran were examined for LAB. After isolation, the identification of Lactobacilli was done according to traditional and molecular bacteriological tests. Subsequently, a suspension of each bacterium was prepared and the protein content of the cytoplasm was extracted. Cell wall disintegration was done by cell lysis buffer and sonication. The effects of cytoplasmic extraction and cell wall on K562 cell line proliferation were investigated by MTT
assays.
Results: The cytoplasmic extraction of the isolated Lactobacilli had significant (p<0.05)
anti-proliferative effects on K562 cells. The cytoplasmic extractions of Lactobacillus paracasei and Lactobacillus casei inhibited K562 cell proliferation by 66.56% and 54.28% at 83.33 μg/ml concentration, respectively.Nevertheless, the Lactobacillus cell wall could not inhibit the proliferations
of K562 cells (p<0.05).
Conclusion: In this study, the cytoplasmic extractions of the isolated Lactobacilli from the
intestine of common carp had anti-proliferative effects on K562cell line.
Ahangari Aa, Ownagh A, Tehrani A, Tukmechi A,
Volume 69, Issue 1 (4-2011)
Abstract
Background: Propolis (bee glue) is a resinous substance obtained from bee hives
living on various plant sources. The purpose of this study was to evaluate the effects of ethanol extract of propolis (EEP) on the experimentally induced Candidial keratitis in rabbits.
Methods: The alcoholic extract of propolis was prepared by 80% ethyl alcohol. After suppressing the immune system of 24 male rabbits, experimental Candida albicans keratitis was induced in the animals under local anesthesia and sterile conditions. The animals were later divided into four groups including the control or glycerin group and a nystatin and two 500 and 1000µg/ml EEP groups. Treatment continued for 21 days and after sacrificing the animals by humane methods, histopathological samples of the rabbits’ eyes were prepared.
Results: Keratitis was developed in the eyes of all rabbits a week after the yeast inoculation. In the control group in which animals received glycerin, keratitis persisted until day 21. Clinical signs of keratitis disappeared in the Nystatin and 1000µg/ml EEP groups after 14 and 21 days, respectively. The clinical signs of keratitis partially ameliorated in the animals receiving 500µg/ml EEP. Histopathological examination revealed no differences between groups receiving nystatin or 1000µg/ml EEP. Conclusion: It is concluded that, ethanol extract of propolis could completely treat Candida albicans keratitis in 1000µg/ml concentrations. This extract can be used as a safe antifungal agent against Candida albicans and it is a good substitute for synthetic antifungal agents like nystatin.
Malekpour-Dehkordi Z, Javadi E, Doosti M, Paknejad M, Nourbakhsh M, Yassa N, Gerayesh-Nejad S, Heshmat R,
Volume 69, Issue 3 (6-2011)
Abstract
Background: ATP-binding cassette transporter A1 (ABCA1) is a key mediator of
cholesterol efflux to apoA-I in lipid-laden macrophages, the first step of reverse cholesterol transport (RCT) in vivo and a critical step in preventing atherosclerosis.
Enhanced ABCA1 expression may inhibit foam cell formation and consequently reduce atherogenic risk. On the other hand, garlic, Allium sativum, and garlic extracts have been demonstrated to have potential cardiovascular benefits. Moreover, garlic has direct antiatherogenic and antiathersclerotic effects on artery walls. The aim of this study was to evaluate the effects of alcoholic garlic extract on the expression of ABCA1
in macrophages.
Methods: Cell viability assay was used in order to detect the cytotoxic dose of alcoholic garlic extract on macrophages. Real-time PCR and Western blotting were performed to study the effects of alcoholic garlic extract on the expression of ABCA1. Macrophage cells were treated by different concentrations of alcoholic garlic extract for 48 h. The total RNA of the treated macrophages were extracted and analyzed by real-time PCR. ABCA1 protein expression was also analyzed using the Western blotting technique.
Results: Alcoholic garlic extract increased the ABCA1 mRNA (20-23%) and protein expression (18-37%) in THP-1 macrophage cells compared with the controls (untreated cells).
Conclusion: The results of this study are suggestive of the potential effects of alcoholic garlic extract in increasing ABCA1 expression in macrophages, the possibility of promoting reverse cholesterol efflux in macrophages and preventing atherosclerosis
Tanhaye Kalate Sabz F, Farokhi F, Delirezh N, Chapari H, ,
Volume 69, Issue 4 (7-2011)
Abstract
Background: Cell-therapy provides a promising alternative for the treatment of type 1 diabetes. Monocytes which have a reprogramming or differentiation potential and are more available than any other types of stem cells, have been recognized as candidates for such investigations. The aim of the present study was to evaluate the differentiation potential of rat peripheral blood monocytes into insulin-producing cells by the use of rat pancreatic extract (2 days after a 60% pancreatectomy).
Methods: Rat peripheral blood monocytes were isolated and cultured. Adherent monocytes were induced to differentiate into programmable cells in RPMI supplemented by 10% FCS, &beta-mercaptoetanol, M-CSF and IL-3 for six days. The dedifferentiated cells were analyzed by invert microscopy. Cultures of Programmable Cells of Monocytic Origin (PCMOs) were continued in RPMI, containing 10% FBS, pancreatic extract and 5 mmol/L glucose for 15 days. The medium was replaced every three days. At the end of the protocol, insulin and c-peptide excreted by the differentiated cells were tested by radioimmunoassay on days 6, 14, and 21. In order to verify insulin production in the cells, dithizone-staining, which is a method for insulin identification, was employed.
Results: The results showed that the cells cultured in rat pancreatic extract secreted insulin and c-peptide relative to the control group. Dithizone-staining was positive in the aforesaid cells (P<0/05).
Conclusion: The results of the current study showed that pancreatic extract treatment can differentiate rat peripheral blood monocytes into insulin-producing cells which can be regarded as a potential source for the treatment of diabetes.
Asgharian Am, Mohammadi M,
Volume 69, Issue 10 (1-2012)
Abstract
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Background: Amphibian
skins possess various antibacterial compounds that are effective against some
microbial pathogens and are mostly released in response to environmental
stress. In fact, the skin of Rana ridibunda, a large green frog, is a
rich source of antimicrobial compounds that can be developed for therapeutic
use. In the present study, the skin extract of Iranian Rana ridibunda was
evaluated for its antimicrobial, hemolytic and cytototoxic activities.
Methods : The frog specimens were collected from Minoodasht located in Golesten
province in Iran, during 2009. Subsequently, their skins were removed and the intended compounds
were extracted. The crude extract was partially purified by gel filtration
chromatography. The antimicrobial effects of skin extract were assessed against
various microorganisms such as Escherchia coli, methicillin-resistant
and -sensitive Staphyloccus aureus, vancomycin-resistant and -susceptible
Enteroccus fecalis, Pseudomonas aeroginosa and Candida albicans. In
addition, its minimum inhibition concentration, cytotoxic and hemolytic
activities were determined.
Results : The crude extract of Rana ridibunda skin had valuable
antimicrobial effects against methicillin-resistant and -susceptible S. aureus in comparison with E.coli and vancomycin-resistant
and -susceptible E. fecalis. Besides, no
antimicrobial activities were seen against P. aeroginosa or C. albicans. Moreover, the hemolytic and cytotoxic activities of the skin
extract were minimal.
Conclusion: The
antimicrobial activity of Iranian Rana ridibunda was comparable to those
isolated from other Rana species. In conclusion, the skin extract of Rana
ridibunda had the potential for a new therapeutic agent against the emerging
drug-resistant bacteria, particularly methicillin-resistant and -sensitive S.
aureus.
Takzaree N, Mortazavi H, Hassanzadeh G, Safaye S, Hossini M,
Volume 70, Issue 11 (2-2013)
Abstract
Background: Achillea millefolium or yarrow is a native plant in Europe and Iran. Yarrow has been used as a medicine historically, mainly because of its astringent effects. It is reported to be associated with the treatment of several ailments. Nowadays use of plants for medical purpose has become very common. Achillea millefolium L, Yarrow, is being used in traditional and modern medicine due to various chemical compounds. Considering the importance of birth control, finding a drug with less side effects inhibiting spermatogenesis seems to be necessary. The aim of the present study was to investigate the effect of ethanol extract of Achillea millefolium L. on spermato-genesis of male wistar rats.
Methods: In this study, 32 adult male wistar rats were used. The animals were divided to four groups of eight rats. The first group, received 200 mg/kg Achillea millefolium L. interaperitoneally, the second and third groups received 400, 800 mg/kg Achillea millefolium L. interaperitoneally, respectively. In the fourth group (control) distilled water was administered. After 20 days, the rats were sacrificed and testis tissues were histologically evaluated.
Results: Comparing to control group, in the experimental groups received the high doses of the extract, thickening of seminiferous tubules basement membrane, loss of germinal epithelium and testicular hyperemia were demonstrated (P<0.001).
Conclusion: Based on the results, high concentrations of Achillea millefolium L. leaded to structural and spermatogenesis changes in testis tissue.
Loghmanni A, Delirezha N, Ownagh A, Mohebalianc H,
Volume 70, Issue 12 (3-2013)
Abstract
Background: Multiple Sclerosis (MS) is an autoimmune disease with impairment in function of central nervous system. Macrophages and dendritic cells play important roles in alleviating or progression of the disease. These cells can cause inflammation and damage to the myelin of nerve cells by realizing of harmful substances when these cells get matured. We studied the effect of Alternaria alternata extract on maturation of monocyte- derived dendritic cell (modc) and T-cell responses in the presence of Myelin Basic Protein (MBP) as a laboratory model of multiple sclerosis (MS). The purpose of this study is suitable dendritic cells production for usage in MS immunotherapy.
Methods: For this study plastic adherent monocytes were cultured with granulocyte/ macrophage- colony stimulating factor (GM-CSF) and interleukin -4 for converting these cells to modc and pulsed with MBP and matured in the presence of monocyte-conditioned medium (MCM) in control group and MCM + Alternaria alternata extract in treatment groups. Anti-CD14, anti-CD83, anti-human leukocyte antigen-DR (anti HLA-DR) monoclonal antibody were carried out for phenotyping. Autologos T cell responses and cytokine production were evaluated.
Results: The results showed that the expression of CD14 decreased and CD83, HLA-DR increased in treatment groups in comparison with control groups. The production amount of IL-10 overcame IL-12 and in T cell the production of cytokines, IL-17 and Interferon-γ (IFN-γ) decreased and IL-4 was increased (P<0.05). These effects escalated with increasing of dosage from 50 to 100 (mg/ml) (P<0.001).
Conclusion: Alternaria alternata extract can cause maturation of MBP-pulsed modc and skewing of T- lymphocyte toward Th2 and thereby can evolve into a new strategy in immunotherapy of MS.
