Mahmoudi Rad M, Zafarghandi As, Shivaei M, Mahmoudi Rad N, Abbasabadi B, Amel Zabihi M, Amiri Z,
Volume 67, Issue 11 (2-2010)
Abstract
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Background: Vulvovaginal
candidiasis is a common mucosal infection among immunocompetent, healthy women,
and is caused by opportunistic yeasts that belong to genus Candida. In this study, we isolated and identified the Candida species in the vagina of patients who admitted in
Gynecology Department of Mahdieh Hospital in Tehran, Iran to evaluate the in
vitro activities of fluconazole, miconazole, itraconazole and flucytosine against 191 clinical Candida isolates by the NCCLS microdilution method.
Methods: 191 Candida were isolated from vaginal secretions and identified
with conventional mycological methods in the diagnosis
of Candida species. The identity of all strains was confirmed genotypically by multiplex PCR. In vitro
susceptibility testing of vaginal Candida isolates was
performed by the NCCLS broth microdilution
method. The results were read at 48 h.
Results: Most C. albicans isolates (>90%) were sensitive in vitro to the antifungal agents
tested. Most C. glabrata isolates showed sensitivity to miconazole and then flucytosine while they were
more resistant to Itraconazole and fluconazole. Many isolates of C.
tropicalis were susceptible to
miconazole and then fluconazole. They
showed a little resistance to all antifungals tested and flucytosine-resistance
was the most frequent in the C. tropicalis isolates. High susceptibility to miconazole was observed in isolates of C. krusei and their susceptibility to the rest of the
antifungals tested was dose-dependent. fluconazole -resistance was
the most frequent in the C. krusei isolates.
Conclusion: Most isolates tested were susceptible to miconazole. A
trend toward increased resistance among C. glabrata and C.
krusei strains to antifungals tested was noted. Our
findings suggest that, miconazole should be the agent of choice for the treatment of resistant
vaginal candidiasis.
Hashemi Sj, Zaini F, Daie R, Zibafar E, Zakeri Ma,
Volume 69, Issue 2 (5-2011)
Abstract
Background: Different studies have shown that despite the expanding number of antifungal agents, death rate caused by Aspergillus species has been increased during the recent decades due to drug-resistance occurrence, increased minimum inhibitory concentration (MIC) and cross-resistance among the isolated species. Regarding the lack of effective response to conventional treatments and antifungal susceptibility patterns of the most common isolated Aspergillus species, this study was undertaken to draw a clearer picture in the Iranian setting.
Methods: During 13 months from September 2009 to October 2010, 50 clinically isolated Aspergillus cases were identified based on the method described by Klich (2002) and their morphological features. Subsequently, their susceptibility test was carried out according to NCCLS- M38A broth microdilution method.
Results: We found that 7.5% of the isolated A. flavus with an MIC>2 µg/ml to amphotericin B were probably clinically resistant types, and 25% of them with an
MIC<8 µg/ml to itraconazole were less sensitive isolated species. The isolates were less
sensitive to voriconazole too. The MIC range of 9 strains of A. niger and the MIC of one strain of A. fumigatus had increased to all the three medications in comparison with similar foreign studies.
Conclusion: In this study we found that the MICs of most isolates were in the range of the reference strains and the MICs of some isolates were in the range of similar foreign studies. In some significant cases, the MICs were beyond the known ranges showing the lower sensitivity of Iranian isolates and their increased MIC patterns.
