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Shokoufeh Akbari, Monireh Rahimkhani, Reza Mirnejad,
Volume 81, Issue 10 (1-2024)
Abstract

Background: Today, Methicillin-Resistant Staphylococcus Aureus (MRSA) has become one of the public health concerns due to its resistance to antimicrobial drugs, and this problem makes treating patients with infections caused by this bacterium difficult. Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) strains are pervasive in both community and hospital settings, primarily attributable to Staphylococcus aureus' capacity to colonize areas like the nose or skin. In this study, with the aim of comparing phenotypic (disc diffusion method) and genotypic (PCR) methods, to detect methicillin-resistant Staphylococcus aureus isolated from patients of hospitals under supervision of Tehran university of medical sciences, and also detection of nor A, that is the one of the most important genes in efflux pump cluster genes.
Methods: The present research was a cross- sectional study that was conducted from February 2022 to September 2023. In this research, 43 isolated strains of Staphylococcus aureus from wound discharge and blood samples, were collected from different departments of Tehran hospitals and had submitted to the research laboratory of the school of allied medical sciences in Tehran university of medical sciences. After identifying the strains, the resistance of the isolates to 14 types of antibiotics was checked by disk diffusion method.
Results: Staphylococcus aureus diagnostic tests including gram staining on colonies, catalase, coagulase, DNase tests were performed and it was found that all strains were Staphylococcus aureus. In the next step, all samples were resistant to Cloxacillin by disc diffusion method, and the presence of mec A gene in them was confirmed by PCR method, thus the presence of MRSA strains was confirmed from the genotypic point of view. Of the 43 Staphylococcus aureus strains, 26 samples were identified as having the nor A gene by PCR and electrophoresis.
Conclusion: The results of the present research have shown that the prevalence of Staphylococcus aureus bacteria in hospital samples is significant and resistance to methicillin and ciprofloxacin has increased in the strains of this bacteria.

Mahdieh Soltani , Seyyede Zohreh Seyyedsalehi, Reyhane Mahdavi,
Volume 82, Issue 9 (12-2024)
Abstract

With the rapid expansion of artificial intelligence across clinical disciplines, a variety of artificial neural networks (ANNs) have become indispensable tools for endowing computer systems with advanced analytical power. Dentistry, as an informationrich branch of medicine, routinely generates and must interpret large, complex datasets from imaging and diagnostic records. Consequently, researchers have increasingly directed their attention toward intelligent, automated techniques for analyzing dental data. This study therefore surveys and synthesizes the methods that have been applied to the intelligent and automated analysis of such data, highlighting the prevailing trends in current literature.The majority of the examined investigations relied on panoramic radiographic images of the teeth orthopantomograms (OPG) as their primary source material. Three overarching technical objectives repeatedly emerged: first, tooth diagnosis, meaning the reliable separation and identification of each individual tooth from its neighbors; second, sample segmentation, that is, the piecebypiece analysis of visual information within the image; and third, semantic segmentation, namely, the contextual interpretation of information extracted from the radiograph. Depending upon which of these objectives was pursued, researchers selected different neuralnetwork architectures and configurations. Across the reviewed corpus, input images were typically subjected to preprocessing steps such as normalization, noise reduction, and contrast enhancement before being supplied to a neural network for training, thereby preparing the data for subsequent machine interpretation. In several instances, the raw output produced by the neural network underwent additional postprocessing, a stage designed to refine the preliminary results and enhance overall accuracy. The comparative analysis presented here concentrates on how effectively the various neuralnetwork models fulfilled the three technical objectives described above. The surveyed articles reveal two dominant analytical approaches. In the intelligent problemsolving paradigm, convolutional neural networks (CNNs) overwhelmingly predominate. Conversely, in the automated paradigm, investigators favor classical, nonlearning algorithmic techniques. Work employing ANNs consistently emphasizes image comprehension, segmentation, feature extraction, feature classification, network modeling, and careful variable tuning to promote effective learning that aligns with each study’s stated objectives.


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