Showing 6 results for P53
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Volume 66, Issue 4 (7-2008)
Abstract
Background: Ewing's sarcoma is one of the most malignant tumors in children and young adults. Only a few established cell lines of Ewing's sarcoma have been reported, which makes it difficult to study the biological features of these tumors. We have recently established a new Ewing's sarcoma cell line designated SS-ES-1, originating from a thoracic tumor of a 16-year-old female patient. The SS-ES-1 cells have been grown continuously in culture for over 90 passages. In this report, some characteristics of SS-ES-1 cells are presented.
Methods: The cells were grown in DMEM medium supplemented with 10% fetal bovine serum (FBS), 100 mg/ml streptomycin and 100 U/ml penicillin in a humidified atmosphere with 7% CO2 at 37 ºC. The cells were immunocytochemically characterized using a panel of monoclonal and polyclonal antibodies. Furthermore, the chemo-sensitivity of this cell line to some anticancer drugs was assessed using MTT assay and IC50 values were determined.
Results: Morphologically, the SS-ES-1 cell line is composed of poorly differentiated small round cells growing in a multilayer pattern. The immunocytochemical staining demonstrates strong reactivity to CD99, cytokeratin, neurofilament, p53 and Ki67 proteins, but no reactivity to GFAP. Based on IC50 values, SS-ES-1 cells display considerable sensitivity to vinblastine (2±0.7 pM), followed by vincristine (0.3±0.12 nM), doxorubicin (0.05±0.03 µM), etoposide (0.64±0.28 µM) and cisplatin (0.67±0.45 µM).
Conclusions: In conclusion, the SS-ES-1 cell line demonstrates unique cellular properties, which make it a useful model for studying various aspects of the biology of Ewing's sarcoma.
Fouladdel Sh, Mohammadi-Karakani A, Ghazi-Khansari M, Azizi E,
Volume 66, Issue 7 (10-2008)
Abstract
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MicrosoftInternetExplorer4
Background: Paraquat
is an herbicide produced and used prevalently worldwide. Studies have shown
that lung fibrosis induced by paraquat can be prevented or delayed by certain
antioxidants, iron chelating agents, melatonin, and, recently, blood pressure
lowering drugs such as captopril.
Methods: The protective effects of captopril on paraquat
toxicity were studied using RT-PCR and immunohistochemistry to determine the gene and
protein expression of p53 and Bcl-2 in lung tissue samples from rats treated with captopril
before and after exposure to paraquat.
Results: We found no significant difference in the gene and
protein expression of p53 in different tissue samples, except for mRNA levels in the
lung tissue of captopril-treated rats. However, the protein expression of Bcl-2 is greater in tissue
from rats exposed to paraquat alone and paraquat together with pre- and
posttreatment with captopril compared to tissue from untreated control rats and
from those treated with captopril alone, which can be due to inflammatory
responses of lung tissue. By RT-PCR, we were unable to detect Bcl-2 in lung tissue
samples.
Conclusion: These results show that paraquat does not induce
significant DNA
damage therefore, the gene and protein expression of p53 was not changed.
Paraquat does induce lung tissue inflammation, which in turn increases Bcl-2 protein expression.
Finally, captopril had no significant effect on the lung tissue toxicity
induced by paraquat. Considering these results and the cellular interactions in
lung tissue, we suggest that complementary assays and in-vitro cell culture experiments be performed to further elucidate
the molecular events underlying paraquat lung toxicity.
Madani Kermani Z, Khorsandi Mt, Yazdani N, Mirashrafi F,
Volume 67, Issue 7 (10-2009)
Abstract
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Background: Neck
lymph node metastasis has the prognostic role in SCC of the tongue and the
importance of the biologic markers in tumor invasion and metastasis has been
stated in the medical literature. The aim of this study was to evaluate the
relationship between two biomarkers, p53 and EGFR (which had the main role in cell proliferation) and
two other biomarkers, CD44 and E-cadherin, in lymph node metastasis.
Methods: In an analytic descriptive study fifty three patients
with SCC (Squamous Cell Carcinoma) of the tongue who underwent the resection of
tumor and dissection of neck lymph nodes were assessed during the year of 2002-2009.
Histological samples from 53 patients were immunohistochemically stained and the
analysis of these markers were performed due to clinicopathological variable
and metastasis of the neck lymph nodes.
Results: The result showed that among the clinicopathological
factors, the relationship between Age (p=0.01), history of having risk factors (p=0.002), clinical
lymphadenopathy (p=0.002), the size of the tumor (p=0.001),
decreasing of CD44 (p=0.02) and lymph node metastasis of
the neck were statistically significant. No significant relationship were found
between sex and other biomarkers including p53, EGFR, E-cadherin.
Conclusion: CD44 is an important
indicator of prognostic markers that can also be used as an indicator of
clinocopathological markers.
Bagheri Hossein-Abadi Z, Rajabalian S, Kalantari-Khandani B, Poya F, Saleh Moghaddam M, Motamedi B,
Volume 69, Issue 3 (6-2011)
Abstract
Background: Ewing sarcoma family tumors (ESFTs) are among the most malignant tumors in children and young adults. ESFTs include Ewing sarcoma (ES) and peripheral primitive neuroectodermal tumors (pPNETs). As there seemed to be few studies on the molecular biology of ESFTs, we investigated the frequency of CD99, Ki67, p53 and Fli- 1 protein expression in 15 Iranian patients with ESFTs. In addition, the correlation between expression rate of these proteins and various clinical factors, including age, sex and survival was computed.
Methods: The expression of the aforesaid proteins was studied by immunohisto- chemistry in formalin-fixed and paraffin-embedded blocks of 15 ESFTs specimens. Stained sections were classified according to the percentage of stained tumor cells. Results: The results showed the membrane expression of CD99 protein in all of the specimens. The nuclear expression of Fli-1 protein was observed in 86.7% and the over- expression of p53 nuclear protein was seen in 53.3% of the specimens. The expression rate of Ki67 protein was 60%. Although a significant correlation was not shown between the expression levels of Ki67, p53 or Fli-1 proteins with age, sex or survival of the patients, there was a significant correlation between expression levels of p53 and Ki67 proteins (P=0.003).
Conclusion: The results underline the role of p53 and Ki67 proteins in the development and progression of ESFTs and suggest the simultaneous immunohistochemical staining of Fli-1 and CD99 proteins for the diagnosis of ESFTs.
Narges Izadi-Mood, Soheila Sarmadi , Banafsheh Rajabian , Fariba Yarandi , Afsaneh Rajabiani ,
Volume 71, Issue 10 (1-2014)
Abstract
Background: Recently the use of “two tier" grading system in which ovarian serous carcinoma was classified as low-grade or high-grade in comparing to preceding system has improved authority in prognosis and survival. This approach is simplistic, reproducible, and based on biologic evidence. In this study, we reclassified ovarian serous carcinoma by a new two-tier system for grading and then evaluation of P53 expression in these tumors by immunohistochemistry method.
Methods: We retrospectively reviewed 32 cases of ovarian serous carcinoma with previous diagnosis of well differentiated (eight cases) and moderate to poorly differentiated serous carcinoma (24 cases) and according "two tier" grading system in low-grade vs. high-grade serous carcinoma reclassified. Subsequntly all cases immunostained by P53 marker. Also clinical data related to survival of patients (with or without recurrence of tumor and death) and paraclinical findings such as presurgical blood serum level of CA125 are gathered.
Results: Out of total eight patients with previously diagnosis well diferentiated serous carcinoma and of 24 patients with moderate to poorly differentiated serous carcinoma reclassified as low-grade and high-grade ovarian serous carcinoma respectively and a statistically significant difference was found between two groups. (P<0.005) Also of total 24 cases with high grade serous carcinoma, in 12 cases (54%) P53 immunostaining was detected but in non of all low grade serous carcinoma was seen. All 8 low grade serous carcinoma were alive without recurrence of tumor. In 10 and 12 out of 24 cases with high grade serous carcinoma recurrence of tumor and death were seen respectively.
Conclusion: Since the presence of P53 negative expression in all of low-grade serous carcinoma by immunostaining and low-grade serous carcinoma accounts for small pupulation of all ovarian serous carcinoma and also few cases in our study, we did not find significant differences between P53 expression and survival in two low-grade vs high-grade serous carcinoma groups.
Hossein Shirvani , Amin Isanejad , Mostafa Rahimi , Behzad Bazgir , Ali Mohammad Alizadeh ,
Volume 76, Issue 5 (8-2018)
Abstract
Background: Recent evidence suggests that regular exercise training is effective in treating various aspects of cancer. Therefore, the purpose of this study was to determine the effect of 8 weeks of aerobic interval training on monocarboxylate transporter 1 (MCT1) protein and expression of p53 gene in tumor of colon cancer mice.
Methods: The present study was conducted experimentally from May to October 2014 at the Exercise Physiology Research Center of Baqiyatallah University of Medical Sciences, Tehran, Iran. Twenty BALB/c mice of age 3 weekly with a mean weight of 17.6±1.4 grams were selected and randomly divided into 4 groups: control (N=5), interval training (N=5), colon tumor (N=5) and interval training+colon tumor (N=5). The cancer was induced by subcutaneous injection of a carcinogenic azoxymethane (10 mg/kg) once a week for three weeks, and aerobic exercise was performed with rodent treadmill for 8 weeks and 5 days a week. Forty-eight hours after the last training session, the mice were cleared and colon removed. Measurement of MCT1 protein was performed by ELISA and commercial kits (ZellBio, Germany). Real-time polymerase chain reaction (PCR) was used to determine the relative expression of p53 gene. Data were analyzed by Kruskal-Wallis test and Mann-Whitney U tests.
Results: The results showed a significant increase in MCT1 protein (P< 0.01) and significant reductions in p53 gene expression (P< 0.001) in a colon tumor group compared to other groups. Also, there was a significant decrease in the level of MCT1 protein (P< 0.01) and significant increase in p53 gene expression (P< 0.001) in the exercise training group and exercise training+colon tumor group compared to control group and the tumor group was observed.
Conclusion: The findings of the study showed that aerobic interval training reduced the protein content of MCT1 and increased the expression of p53 gene (as a tumor inhibitor) in the tumor of colon cancer mice. These factors are portions of the mechanisms involved in cancer cell metabolism by which aerobic interval training shows part of its therapeutic effect in colon cancer.
