Sh Niroomanesh, Gh Behzadiannezhad, M Ebrahimi Torabi,
Volume 56, Issue 1 (3-1998)
Abstract
Between 270 patients complaining of symptoms of vaginitis, bacterial vaginosis was diagnosed in 68 women (%25) based on at least three of four criteria: 1. Homogenous vaginal discharge 2. Positive amine test 3. pH more than 4.5 4. Clue cell in wet smear. The results were compared to 55 normal controls.
Clue cell with more than %20 of epithelial cells was seen in %87 of cases and %5.5 of controls. A pH more than 4.5 showed the greatest sensitivity (%100) and the smallest specificity (%47). The most frequent complaints included increased discharge, bad odor, and sensing bad odor after coitus.
N Rahimifard , M Ahi , A Kahnamooei ,
Volume 57, Issue 4 (7-1999)
Abstract
The object of this study was to find a suitable staining method for P.carinii. This parasite is not easily stained and clinical signs are not specific for the diagnosis of P.carinii pneumonia and therefore optimal laboratory methods for observing the organism are extremely valuable. In all 17 new conventional and modified staining techniques were used on lung impression smears and tissue section of sprague Dawley Rat treated with cortisone. Of these methods modified methylene blue 1 & 2, modified cresy violet 1, 2, 3, 4 modified Gram, modified Giemsa 2 and modified Griedley techniques have not previously been reported. After comparing readability of the slides, ease of performances, rapidity, availability and sensitivity of these 17 techniques for the diagnosis of P.carinii pneumonia, modified toluidine blue 01 & 2, modified methylene blue 1 & 2 and modified cresyl violet 3, 4 are suggested as the methods of choice for the rapid diagnosis of P.carinii pneumonia.
Mehdi Golchin, Fatemeh Noori, Ali Akbar Khalili-Yazdi,
Volume 67, Issue 12 (3-2010)
Abstract
Normal
0
false
false
false
EN-US
X-NONE
AR-SA
MicrosoftInternetExplorer4 !mso]>
ject classid="clsid:38481807-CA0E-42D2-BF39-B33AF135CC4D" id=ieooui>
Background: Recombinant antibodies are new versions of
monoclonal antibodies that are produced by recent molecular biology techniques.
These antibodies can be isolated by phage
display technology from immune or non-immune libraries. Recombinant
antibodies are applied to treatment of some diseases and also are increasingly
used for diagnosis and detection of many antigens. In
the latter case, the presence of antigen-antibody complexes has to be detected
by further approaches. The aim of current
research was to stain an anti-K99 phage antibody with
two different protein dyes and to apply them directly for detection of E.
coli K99
fimbriae.
Methods: In order to stain above antibody, a phagmid
vector carrying the anti-K99 single-chain Fv
(scFv) antibody was isolated, purified and
transformed into TG1 strain of E.
coli. Afterward,
the antibody was expressed in this cell as phage-scFv
antibody. Phage antibodies were
subsequently eluted, purified and stained with Disperse
Red dye 60
and Coomassie Brilliant
Blue. Finally,
the binding activity of coloured phage antibodies towards the purified K99
fimbriae was verified by immunoblotting.
Results: The results showed that anti-K99
phage antibody was stained with both dyes and the coloured phages were able to
recognize the corresponding antigen.
Conclusions: These protein stains that they usually do not
alter the protein structure can be used for staining phage antibodies. The
coloured phage antibodies retain their binding affinity for the antigens, and
therefore can be applied to detection of relevant antigens.
