Abdolmaleki A, Zahri S, Bezaatpour A,
Volume 71, Issue 1 (4-2013)
Abstract
Background: Salen metal complexes are used successfully in a wide range of asymmet-ric reactions and important in the pharmaceutical and industry. On the toxicity of salen vanadium oxide (VOsalen) on embryo and cell cultures, little information is available. In the present study, the toxic and teratogenic effects of VOsalen was evaluated against chicken embryos as a animal model and liver and fibroblast cell cultures which was derived from the embryo.
Methods: The VOsalen compound was synthesized. The compound solution was inject-ed in triplicate examination, in the air sac of the eggs, at third day of incubation. Treat-ed and control eggs, on day 19 of incubation opened and embryos were weighted, then mortality rate was recorded. The liver and fibroblast cell culture were treated by this and survival fraction was recorded.
Results: The survived fraction of the embryos depends on the compound concentration. In concentration of 300μM/egg, 36/32% of the embryos survived and the Lethal dose 50% (LD50) was 226/37 μM/egg. Morphological study of the treated embryos showed retarded growth, and skeletal staining showed the deletion of caudal vertebrate. The compound was inhibited liver and fibroblast cells growth with IC50 1047/25 and 1036/82μM respectively. The cytoplasm of treated cells became dense and their interco-nnections were loosed.
Conclusion: The VOsalen compound had low toxic effects against the embryos and the cultured cells at the concentrations. Significant cytotoxic effect was not observed in the treated cells. However the proliferative cells were affected significantly in comparison with the cells which their growth was stopped. The effect of VOsalen compound against replication of liver cells were lower than fibroblast cells.
Iraj Ragerdi Kashani , Mohammad Ansari , Kobra Mehrannia , Kasra Moazzemi , Safura Vardasbi Joybary ,
Volume 71, Issue 8 (11-2013)
Abstract
Background: A number of studies on reproduction have mentioned Origanum Vulgare extract’s ability to reduce mortality rates and improve fertility rates. However, other studies have suggested that it is possible to use Origanum Vulgare extract to induce abortion. The aim of this study was to investigate the effect of different doses of Origanum Vulgare on embryo survival and macroscopic abnormalities in mice.
Methods: In this study, 24 mice Balb/c female weighting approximately 25-30 g were divided into 4 groups. Origanum Vulgare extract was prepared different concentrations (2.5, 12.5, and 25 mg in 0.25 ml distilled water) were administered, by oral gavage, to three experimental groups of mice between day 6 (starting gastrulation) until day 15 of pregnancy (end of organogenesis). The control group consisted of six mice that received 0.25 ml of distilled water daily. On day 16 of study, pregnant mice were anesthetized by chloroform and fetuses were removed and stained with Alcian Blue, Alizarin Red s and microwave irradiation. Morphological and skeletal abnormalities were investigated by light and stereomicroscopes.
Results: The results of this study showed that high doses of the Origanum Vulgare extract significantly decreased the mean number of embryos (100.5, P>0.05), mean number of live embryos (70.5, P>0.05) in each mouse and resulted in significant reduction in mean weight(11848 mg, P>0.05) and crown-rump length(11.90.23 mm, P>0.05) and the overall size of fetuses compared to control group, whereas there was no significant difference between the groups receiving low dose of Origanum Vulgare extract with control group. In addition, under the effect of the Origanum Vulgare extract the subcutaneous bleeding seemed (20.1, P>0.05) significantly more frequent compared to the control group.
Conclusion: Origanum Vulgare extract did not have any positive effect on fetal development and high dosages led to an increased incidence rate of abortion and fetal malformations in the fetuses of women who received it.
