Showing 6 results for Urine
M Soleimani, S Nadri , R Izadpanah ,
Volume 66, Issue 4 (7-2008)
Abstract
Background: MSCs have been isolated from a variety of mammals by the plastic adherence method. However, this method can be problematic due to the unwanted growth of hematopoietic cells and non-MSCs. The potential of MSCs to differentiate along multiple lineages is the key to the identification of stem cell populations in the absence of molecular markers. In the present study, we describe a homogeneous population of MSCs from mouse bone marrow isolated using an improved plastic adherence method that employs frequent medium change (FMC) at the initial hours of harvested bone marrow cell culture.
Methods: Balb/c mice were sacrificed and whole bone marrow cells were aspirated from the femur and tibia and then cultivated in six-well plates. After 3-4 hours of culture, old medium was removed and fresh medium was added. FMC was performed every eight hours over a 72 hour period. When primary cultures became nearly confluent, the first passage was performed. These cells were then used for further examination. To investigate their mesenchymal nature, the cells were allowed to differentiate into mesenchymal lineages and examined at each passage up to the tenth passage for surface antigens by flow cytometry.
Results: We achieved purified populations of fibroblast-like cells in the two weeks after culture initiation. The cells were capable of differentiating into osteocytes and adipocytes. Isolated MSCs were reactive to the CD44, Sca-1, and CD90 cell surface markers. MSCs were negative for hematopoietic surface markers such as CD34, CD11b, CD45, CD31, CD106, CD117 and CD135.
Conclusions: This protocol provides an efficient isolation of homogeneous populations of MSCs from mouse bone marrow.
Chamani-Tabriz L, Tehrani Mj, Zeraati H, Asgari S, Tarahomi M, Moini M, Ghasemi J,
Volume 66, Issue 7 (10-2008)
Abstract
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Background: Chlamydia
trachomatis is a common and curable STI that may be
symptomatic or asymptomatic. The few studies on C. trachomatis among Iranian women have
had, for the most part, small sample sizes and are therefore unsuitable for
epidemiological deductions. The aim of this study was to estimate the
prevalence of urogenital C. trachomatis infections by PCR on urine samples of married women in their
fertile years in order to determine the need for a C. trachomatis screening program for
asymptomatic women in Iran.
Methods: This descriptive-analytical and cross-sectional study
was performed on 991 married women. The research material consisted of
questionnaires and urine samples, which were transported daily to Avesina
Research Institute, Tehran,
Iran, to
extract their DNA and prepare them for PCR tests. The gathered data were
analyzed by SPSS, version 13, and evaluated statistically by t-test, chi-square
test, Fisher's exact test and logistic regression, considering p<0.05 as
significant.
Results: Of all the subjects, 127 (12.8%) were positive by PCR for C. trachomatis.
The mean age of the participants was 28.88±
6.19 years. Infection was more prevalent among
those with lower levels of education, who were employed and not pregnant. This
infection was more prevalent among those who were using contraception, especially
condoms. Reproductive history revealed that infection was more prevalent among
participants with a history of vaginal discharge, pelvic pain, infertility and
low birth-weight infants, and less prevalent among those with a history of abortion,
preterm delivery and ectopic pregnancy. However, these patterns were not statistically
significant.
Conclusion: In populations with C. trachomatis prevalences higher than 4%,
screening programs are recommended. Thus, Chlamydia screening should be part of the health care program in Iran to reduce the
burden of this disease.
Yahyazadeh Sr, Mehraban D, Ghaffari Sh, Alimoghadam K, Ghavamzadeh A, Naderi Gh, Kazemeyni Sm, Rasteh M,
Volume 67, Issue 1 (4-2009)
Abstract
Background: Transitional Cell Carcinoma (TCC) of bladder is the second most common urogenital malignancy and because of its high rate of recurrence (two third of tumors recur) vigilant surveillance is necessary. There have been a lot of efforts to find a proper biomarker for detecting urothelial cancers because available methods are expensive and invasive (like cystoscopy) or have a low degree of sensitivity (like urine cytology). Urothelial malignancies, like other cancers tend to express a large amount of telomerase. The aim of this study was to evaluate the possible application of voided urine human telomerase reverse transcriptase (hTERT) mRNA assay in detecting low-grade bladder carcinoma in comparison with urine cytology.
Methods: Voided urine samples were collected from 49 patients who were supposed to go under operation. Samples were examined by both Quantitative Real-time RT-PCR (for measuring hTERT mRNA level) and cytology the results were then compared to the final pathologic studies.
Results: Regardless of clinical stage and or pathological grade of tumor, sensitivity of telomerase test and urine cytology was 74% and 16% respectively. There was a strong correlation between results of urine cytology and stage and/or grade of tumor however, sensitivity of telomerase test was acceptable regardless of stage and or grade of tumor. There was a statistically significant difference between sensitivity of urine cytology and telomerase test (p<0.001).
Conclusion: Detection of hTERT-mRNA can potentially be used as a non-invasive method for diagnosis and follow up of bladder carcinoma instead of urine cytology.
Khazardoost S, Noorzadeh M, Abdollahi A, Mirrokni Sm,
Volume 68, Issue 5 (8-2010)
Abstract
Background: Preeclampsia, a specific syndrome in pregnancy, may summits mortality or morbidity in mother and fetus. Diagnostic methods are based on 24 hours urine protein measures which may be tedious, thus it is desirable to apply a faster and more applicable method for this goal. In this study we evaluate measurement of 8-hours urine protein in order to estimate 24-hours urine protein measure.
Methods: Fifty pregnant women were entered in a cross sectional study in Vali-e-asr hospital located in Tehran- Iran, during 2008-2009. A urine sample was given after 8-hours and urine volume as well as protein levels were calculated and compared with the same values of 24-hours urine measures. Other necessary data was obtained by history taking and physical examination as well as using patient's medical records.
Results: Mean of age was 27.5±5.4 years and mean of gestational age was 30.5±4 weeks. Mean of urine protein in 8 and 24 hours urine protein were 79±119 and 532±1218 mg respectively. Sensitivity, specificity, positive predictive value and negative predictive value of 8 hours urine protein were 61%, 98%, 88% and 90% respectively, in order to diagnosis of 24 hours urine protein to consider cut off point 105 mg for 8 hours urine protein. Mean protein levels were significantly higher in group with proteinuria ≥300 mg/24h in these two types of urine samples (p< 0.001).
Conclusion: Sensitivity of 8-hours urine protein is low but its specificity is suitable for normal mothers. We offer measuring of 8-hours urine protein as a valuable method for diagnosis of preeclampsia.
Najafi Mr, Sonbolestan F, Aghaghazvini Mr, Sonbolestan Sa,
Volume 68, Issue 12 (3-2011)
Abstract
Background: Diagnosis of multiple sclerosis (MS), as a major cause of neurological disability in young adults, is difficult to establish, especially at the onset of the disease process, due to lack of reliable molecular markers.The goal of the present study was to evaluate serum and urinary concentrations of cystatin C and to find their relationship with patients' expanded disability status scale (EDSS).
Methods: Based on McDonald's criteria, 54 adult patients with M.S.(11 males and 43 females,
with a mean age of 32.18±8.37 years) were enrolled as the case group and 24 age and sex-matched healthy, non-M.S. individuals (7
males and 17 females, with a mean age of 34.31±10.07 years) were recruited as the controls. Serum and urinary concentrations of cystatin C
were measured in all the participants.
Results: The means of serum cystatin C concentrations (mg/Lit)
in the case and control groups respectively were 0.90±0.01 and 0.89±0.02, (p=0.84) and the means for its urinary concentrations were 25.37±1.91
and 21.11±2.54 (p=0.18).The means of serum and urinary cystatin C concentrations were 0.90±0.01 and 25.11±2.33 in patients whose EDSS was ≤2.5
and 0.90±0.03 and 26.30±2.84 in patients whose EDSS was ≥2.5,respectively, although, the differences between the two groups of patients were
not statistically significant (p=0.80 and 0.74,respectively for serum and urinary concentrations of cystatin C).
Conclusions: This
study showed that serum and urinary cystatin C concentrations cannot be used for multiple sclerosis diagnosis or even as a marker in its treatment follow ups or for the determination of disease
severity.
Razaghy-Azar M, Tabatabaei F,
Volume 70, Issue 7 (10-2012)
Abstract
Background: Gitelman syndrome is a rare autosomal recessive disorder that typically presents with recurrent muscle cramps, carpopedal spasms, hypokalemic metabolic alkalosis, hypocalciuria and hypomagnesemia and high urine magnesium during adolescence. Mutation in the gene encoding for sodium chloride co-transporter in distal convoluted tubule causes electrolyte imbalance.
Case presentation: We present a 10-year-old boy complaining of carpopedal spasms, tingling of fingers and facial parestesia for three years prior to his admission in endocrinology clinic of H. Ali-Asghar Pediatric Hospital. The patient had metabolic alkalosis, hypokalemia, hypocalciuria, increased urine fraction excretion of Mg, serum magnesium of 1.8 mg/dl, normal serum calcium and phosphorus and normal blood pressure. His clinical manifestations recovered after potassium and magnesium administration.
Conclusion: A patient with Gitelman syndrome with normal serum Mg. is presented.
