Volume 25, Issue 1 (4-2025)
ijdld 2025, 25(1): 35-43 |
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Sohrabi R, Mousavi S N, Shapouri R, Heidarzadeh S, Shokri R. Relationship Between the Gut Actinobacteria and Bifidobacterium Population with Macronutrient’s Intake in Diet of Mothers with Gestational Diabetes Compared to the Healthy Mothers: A Case-Control Study. ijdld 2025; 25 (1) :35-43
URL: http://ijdld.tums.ac.ir/article-1-6358-en.html
URL: http://ijdld.tums.ac.ir/article-1-6358-en.html
1- Department of Microbiology, Islamic Azad University, Zanjan Branch, Zanjan, Iran
2- Department of Nutrition, School of Public Health, Zanjan University of Medical Sciences, Zanjan, Iran ,neda.mousavi@zums.ac.ir
3- Department of Microbiology and Virology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
2- Department of Nutrition, School of Public Health, Zanjan University of Medical Sciences, Zanjan, Iran ,
3- Department of Microbiology and Virology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
Abstract: (1965 Views)
Background: The Actinobacteria and Bifidobacterium population, which are the beneficial intestinal bacteria, was compared between pregnant women with gestational diabetes (GDM) and healthy-one, and their relationship with the amount of dietary macronutrients was investigated.
Methods: Fifty pregnant women with GDM, who were identified in the 24-28 weeks of pregnancy, based on the oral glucose tolerance test, and 50 healthy pregnant women with normal results were selected. Stool samples were collected. Bacterial DNA was extracted and 16S rRNA gene amplification was done by qPCR method, using universal bacterial primers. Food information was collected using a food frequency questionnaire.
Results: In total, Actinobacteria phyla (P= 0.02) and Bifidobacterium (P= 0.001) were significantly lower in the pregnant women with GDM than the normoglycemic. Adjusting to all factors and grouping, age (P= 0.02), and education (P= 0.04) showed a significant effect on the gut Actinobacteria population. Higher daily calorie intake decreased the intestinal Actinobacteria population by 11.1 times (P=0.01). Dietary carbohydrate and fat showed a positive effect on the gut Actinobacteria (P= 0.02 and P= 0.003, respectively). Dietary cholesterol showed a negative effect on the Bifidobacterium population (OR= -0.54, P= 0.02).
Conclusion: Increase in dietary carbohydrates and fat with an emphasis on mono- and poly-unsaturated fatty acids, decrease in daily calorie intake and cholesterol lead to increase in the Actinobacteria and Bifidobacterium, respectively as the beneficial gut bacteria.
Methods: Fifty pregnant women with GDM, who were identified in the 24-28 weeks of pregnancy, based on the oral glucose tolerance test, and 50 healthy pregnant women with normal results were selected. Stool samples were collected. Bacterial DNA was extracted and 16S rRNA gene amplification was done by qPCR method, using universal bacterial primers. Food information was collected using a food frequency questionnaire.
Results: In total, Actinobacteria phyla (P= 0.02) and Bifidobacterium (P= 0.001) were significantly lower in the pregnant women with GDM than the normoglycemic. Adjusting to all factors and grouping, age (P= 0.02), and education (P= 0.04) showed a significant effect on the gut Actinobacteria population. Higher daily calorie intake decreased the intestinal Actinobacteria population by 11.1 times (P=0.01). Dietary carbohydrate and fat showed a positive effect on the gut Actinobacteria (P= 0.02 and P= 0.003, respectively). Dietary cholesterol showed a negative effect on the Bifidobacterium population (OR= -0.54, P= 0.02).
Conclusion: Increase in dietary carbohydrates and fat with an emphasis on mono- and poly-unsaturated fatty acids, decrease in daily calorie intake and cholesterol lead to increase in the Actinobacteria and Bifidobacterium, respectively as the beneficial gut bacteria.
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