Ethics code: IR.MEDILAM.REC.1402.072
1- Assistant Professor, Department of Gastroenterology and Hepatology, School of Medicine, Razi Hospital, Ilam University of Medical Sciences, Ilam, Iran
2- Professor, Department of Microbiology, Clinical Microbiology Research Center, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran
3- General Physician, School of Medicine, Razi Hospital, Ilam University of Medical Sciences, Ilam, Iran ,nosratpoorgolnaz@yahoo.com
2- Professor, Department of Microbiology, Clinical Microbiology Research Center, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran
3- General Physician, School of Medicine, Razi Hospital, Ilam University of Medical Sciences, Ilam, Iran ,
Abstract: (18 Views)
Background and Aim: Helicobacter pylori is one of the most common causes of human infection. Most infected individuals develop chronic active gastritis, which can lead to gastric ulcers, atrophic gastritis, gastric cancer, and gastric MALT lymphoma. Antibiotic resistance is a multifaceted problem and encompasses medical, social, economic, and anthropological domains. The emergence of antibiotic-resistant strains in clinical settings has raised concerns among public health authorities. The aim of this study was to determine the antibiotic resistance of Helicobacter pylori to amoxicillin, levofloxacin, metronidazole, and clarithromycin in the gastroenterology clinic of Razi Hospital, Ilam.
Materials and Methods: This is a descriptive study conducted over a period of 1 year on patients suspected of having infection caused by Helicobacter pylori who were diagnosed and treated in Razi Hospital as an inpatient or outpatient. In this study, a group of 50 people suspected of having gastrointestinal infections were selected for endoscopy. The obtained categorical data were analyzed using Chi-square and Fisher’s exact tests, while continuous variables (age) were analyzed using Student’s t-test in SPSS software, with a significance level of P<0.05.
Results: A total of 50 patients were studied. None of the 50 patients had taken antibiotics during the previous month. Of these, 25 (50%) were men and the rest were women. The mean age of men in this study was higher than that of women, but this difference was not statistically significant (P-value=0.097). Of the 50 samples collected, 13 were confirmed as Helicobacter pylori based on a combination of positive rapid urease test (RUT), culture, biochemical testing, and genotypic verification via PCR. The PCR test result in all 13 cases was positive. Thus, the prevalence of Helicobacter pylori among patients who had referred to the hospital with gastrointestinal symptoms was 26%. In addition, the test result did not differ statistically significant according to the patient’s gender. Antibiotic sensitivity and resistance testing showed that all 13 samples studied (100%) were resistant to metronidazole and amoxicillin. Ten out of 13 samples (76.9%) were resistant to tetracycline and levofloxacin, and 12 samples (92.3%) were resistant to clarithromycin.
Conclusion: The findings of this study indicated that H.pylori resistance to metronidazole and amoxicillin is high, and resistance to clarithromycin is also significant. Therefore, the choice of treatment regimen should be guided by local resistance patterns. Due to the small number of positive isolates, the results of this study should be interpreted with caution, and larger studies are required for generalization.
Materials and Methods: This is a descriptive study conducted over a period of 1 year on patients suspected of having infection caused by Helicobacter pylori who were diagnosed and treated in Razi Hospital as an inpatient or outpatient. In this study, a group of 50 people suspected of having gastrointestinal infections were selected for endoscopy. The obtained categorical data were analyzed using Chi-square and Fisher’s exact tests, while continuous variables (age) were analyzed using Student’s t-test in SPSS software, with a significance level of P<0.05.
Results: A total of 50 patients were studied. None of the 50 patients had taken antibiotics during the previous month. Of these, 25 (50%) were men and the rest were women. The mean age of men in this study was higher than that of women, but this difference was not statistically significant (P-value=0.097). Of the 50 samples collected, 13 were confirmed as Helicobacter pylori based on a combination of positive rapid urease test (RUT), culture, biochemical testing, and genotypic verification via PCR. The PCR test result in all 13 cases was positive. Thus, the prevalence of Helicobacter pylori among patients who had referred to the hospital with gastrointestinal symptoms was 26%. In addition, the test result did not differ statistically significant according to the patient’s gender. Antibiotic sensitivity and resistance testing showed that all 13 samples studied (100%) were resistant to metronidazole and amoxicillin. Ten out of 13 samples (76.9%) were resistant to tetracycline and levofloxacin, and 12 samples (92.3%) were resistant to clarithromycin.
Conclusion: The findings of this study indicated that H.pylori resistance to metronidazole and amoxicillin is high, and resistance to clarithromycin is also significant. Therefore, the choice of treatment regimen should be guided by local resistance patterns. Due to the small number of positive isolates, the results of this study should be interpreted with caution, and larger studies are required for generalization.
Type of Study: Original Research |
Subject:
Laboratory Sciences
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