Ethics code: IR.MODARES.REC.1401.213
1- Master of Science in Medical Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2- Assistant Professor, Department of Medical Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran ,mahin_tavakoli@modares.ac.ir
3- Associate Professor, Department of Medical Laboratory Sciences, School of Paramedicine, Bushehr University of Medical Sciences, Bushehr, Iran
2- Assistant Professor, Department of Medical Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran ,
3- Associate Professor, Department of Medical Laboratory Sciences, School of Paramedicine, Bushehr University of Medical Sciences, Bushehr, Iran
Abstract: (66 Views)
Background and Aim: Trichophyton rubrum is among the most frequently detected species that cause dermatophyte infections, especially chronic infections, so it requires early diagnosis and appropriate antifungal treatment. Due to the increasing resistance of different species of dermatophytes to antifungals, the use of herbal compounds with antifungal properties can be used as adjuvant therapy or even an alternative therapy. Carvacrol has a wide range of biological activities, including antifungal, antiviral, antibacterial, insecticidal, and antioxidant properties. Accordingly, we aimed to determine the antifungal effect of carvacrol on 12 strains of Trichophyton rubrum in comparison to the antifungal drug terbinafine in vitro.
Materials and Methods: This investigation was conducted on Trichophyton rubrum isolates that had been previously identified morphologically and molecularly. The protocol used to perform in vitro susceptibility testing was the A38-M-CLSI standard using the Broth Microdilution method. The obtained results were analyzed using SPSS software, and the MIC50, MIC90, and GM of this compound against Trichophyton rubrum isolates were estimated compared to terbinafine.
Results: The minimum inhibitory concentration (MIC) was determined using different concentrations of carvacrol (in the concentration range of 0.25 to 2 μg/mL) and terbinafine (in the concentration range of 0.0625 to 0. 5 μg/mL). The MIC50 and MIC90 values for Carvacrol were determined to be 0. 5 and 2 μg/mL, respectively. Whereas, the corresponding values for terbinafine were calculated at 0.0625 and 0. 5 μg/mL. The GM values were 0.5 for cavacrol and 0.1 for terbinafine. Similar to terbinafine, the antifungal efficacy of the studied compound displayed significant differences against Trichophyton rubrum isolates (P-value<0.05).
Conclusion: Although the MIC values for carvacrol were higher than those for terbinafine in vitro, it demonstrated significant antifungal activity against Trichophyton rubrum isolates. Therefore, this natural compound seems to be a good option for adjuvant therapy along with antifungals in dermatophyte infections, although the realization of this matter requires further investigation on the combined effect of carvacrol and terbinafine, determining the toxicity of carvacrol, and also examining this compound in vivo.
Materials and Methods: This investigation was conducted on Trichophyton rubrum isolates that had been previously identified morphologically and molecularly. The protocol used to perform in vitro susceptibility testing was the A38-M-CLSI standard using the Broth Microdilution method. The obtained results were analyzed using SPSS software, and the MIC50, MIC90, and GM of this compound against Trichophyton rubrum isolates were estimated compared to terbinafine.
Results: The minimum inhibitory concentration (MIC) was determined using different concentrations of carvacrol (in the concentration range of 0.25 to 2 μg/mL) and terbinafine (in the concentration range of 0.0625 to 0. 5 μg/mL). The MIC50 and MIC90 values for Carvacrol were determined to be 0. 5 and 2 μg/mL, respectively. Whereas, the corresponding values for terbinafine were calculated at 0.0625 and 0. 5 μg/mL. The GM values were 0.5 for cavacrol and 0.1 for terbinafine. Similar to terbinafine, the antifungal efficacy of the studied compound displayed significant differences against Trichophyton rubrum isolates (P-value<0.05).
Conclusion: Although the MIC values for carvacrol were higher than those for terbinafine in vitro, it demonstrated significant antifungal activity against Trichophyton rubrum isolates. Therefore, this natural compound seems to be a good option for adjuvant therapy along with antifungals in dermatophyte infections, although the realization of this matter requires further investigation on the combined effect of carvacrol and terbinafine, determining the toxicity of carvacrol, and also examining this compound in vivo.
Keywords: Carvacrol, Trichophyton Rubrum, Dermatophyte Infections, Terbinafine, Minimum Inhibitory Concentration
Type of Study: Original Research |
Subject:
Laboratory Sciences
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
