Journal of School of Public Health and

Background and Aim: Type 2 diabetes mellitus is a heterogeneous disorder resulting from a combination of genetic and environmental factors which contribute to pathogenesis by influencing beta cell function and tissue insulin sensitivity. Protein tyrosine phosphatase 1B (PTP1B)" efficiently dephosphorylates the insulin receptor and attenuates insulin signaling. Recently, a 1484insG variant of the PTP1B gene was linked to increased risk of the metabolic syndrome in an Italian population, but this was not confirmed in subsequent studies. The purpose of this research was to investigate the association of 1484insG polymorphism of the PTP1B with obesity, insulin resistance, type 2 diabetes and other cardiovascular-related traits in an Iranian population.

Materials and Methods: The genotypes of 1484insG variant were determined by the PCR-RFLP method in 242 unrelated subjects, including 171 individuals with normal glucose tolerance and normal fasting glucose levels, and 71 type 2 diabetics. Insulin resistance was assessed using the homeostasis assessment model.

Results: The allelic frequency of the 1484insG polymorphism among type 2 diabetic patients and non-diabetic individuals was 6.3% and 3.8%, respectively (p=0.205). None of the subjects were homozygous for the 1484insG allele. Concerning quantitative traits in non-diabetic subjects, carriers of 1484insG allele had significantly higher body mass index (p=0.01), diastolic blood pressure (p=0.012), and HOMA-IR (p= 0.041) levels compared to those carrying the wild-type genotype. In type 2 diabetics, carriers of 1484insG allele had only significantly higher HOMA-B (P= 0/04) level compared to the individuals with the wild-type genotype.

Conclusion: Our results from a sample of Iranian type 2 diabetes cases and controls provide evidence that the 1484insG genotype of the PTP1B gene may be associated with obesity and insulin resistance.

Background and Aim: Rabies, a viral and zoonotic disease, causes acute and fetal encephalitis in humans and other mammals and is a cause of death in developing countries. It is usually transmitted by animal bite, but other routes of transmission are mucus membranes, breathing, placenta, contaminated instruments, and organ implanting. This study shows the mapping of geographical distribution of animal bite cases, rabies, and death due rabies in 3 Caspian Sea littoral provinces, namely, Golestan, Mazandaran, and Gilan.

Materials and Methods: Data on human and animal rabies cases and animal bites were collected from the Reference Rabies Center of the Pasteur Institute in Tehran and the Rabies Diagnosis Section of the Amol Research Center during 2002-2007.The data was analyzed by the SPSS (11.5) software and mapping was done by Arc GIS 9.2.

Results: During the 6-year period, of the 670,743 animal bite cases reported in Iran 63890 (9.5%) had occurred in Golestan, 25,767 (3.8%) in Mazandaran, and 22,874 (3.4%) in Gilan. The distribution of animal bite cases in the 3 provinces was as follows: Agh ghala, Bandar Torkaman, Azad shahr and Kolaleh in Golestan Galugah, Behshahr, Ramsar and Neka in Mazandaran and Shaft, Masal, Siahkal and Fuman in Gilan. The data also showed that of the total 2,312 animal rabies cases, in Iran, 9.22% had been reported from Golestan, 4% from Mazandaran, and 2.6% from Gilan. Furthermore, animal rabies cases had occurred mostly in Gonbadekavoos, Bandar Torkaman, Gorgan and Kolaleh in the Golestan Province Behshahr, Neka and Chalus in the Mazandaran Province and Masal, Fuman and Rezvanshahr in the Gilan Province. The most important disease vector (91.3%) for both human and animal rabies was the dog. A total of 41 human deaths due to rabies had been reported during the period, 2 being from Mazandaran.

Conclusion: The results show that a more effective disease control and surveillance system is essential and can help improve planning for service provision in the health care centers.

  Backgrond and Aim: Demodicosis is a common skin disease. Great number of admissions to the clinics is occurring in the country. Regarding the high prevalence of this parasitic arthropod, investigating on pathogenesis, route of transmission and the complications they may produce, is considered of great importance. Generally, demodex parasites often exist in the skin tissue and tend to live in the face specifically in cheeks, forehead and nose, where sebum excretion is active and may provide a favorable habitat for living and breading the parasite. Diagnosis is carried out during an exploratory skin test for a cne, pityriasis rosacea and ophthalmic infectious diseases such as blepharitis. Determination of their biological role in chronicity of general skin disorders is an interesting topic in research.

  Materials and Methods: Patients were admitted to the Razi hospital, Research Center for skin diseases and leprosy, and three other clinical laboratories considered for assays. The questionnaire was filled for each patient and the sample was taken from the affected area. Ecto parasites were transferred to the potassium chloride solution 10% and studied under a light microscope.

  Results: A total of 100 patients were studied, from which 78 patients were diagnosed as infected with the Demodex folliculorum. Of these, (%92) were female and (%7.6) male (p <0.05). The highest frequency was observed in the age group 41 to 50 years (P <0.05).

  Conclusion: In this study, the relative frequency of demodicosis was more common in women and in the middle-aged group. Generally this high level of frequency will intensify the necessity of the identification of parasites and its pathological effects in chronic infectious diseases.

Background and Aim: Considering the lack of information on the occurrence of the epidemic form of Kaposi’s sarcoma (KS) and the high prevalence of human herpesvirus 8 (HHV-8) infection among human immunodeficiency virus (HIV)-infected patients (46%), it was decided to estimate the incidence of KS in this group. Based on the fact that active HHV-8 infection leads to KS development, it is essential to first assess the prevalence of active HHV-8 infection in these patients. Most of the Iranian HIV-infected patients are not aware that they are HIV-positive. If the prevalence of HHV-8 infection is high in these patients, they may spread HHV-8 in the community by high-risk sexual behaviors, which would lead to an increase in the incidence of classic Kaposi’s sarcoma. The objective of this study was to investigate the prevalence of HHV-8 among HIV-infected subjects.

Materials and Methods: One-hundred plasma samples from HIV-infected patients were collected. Genome was extracted and assessed by the nested PCR assay with specific primers for ORF26. Positive samples were amplified for the ORF K1 region by nested-PCR. Subsequently their products were sequenced and their phylogenic trees constructed.

Results: HHV-8 was detected in 8 of the patients (8%). No statistically significant associations were found between age and gender on the one hand and HHV-8 infection on the other (p > 0.05). Two genotypes, namely, A and C, were identified, the former in two patients and the latter in one.

Conclusion: Although the prevalence of HHV-8 infection is high among Iranian HIV-infected patients, active HHV-8 infection rate is low among them. Therefore, it seems that the incidence of epidemic KS is likely to be very low in this group. Certainly more research is needed in this area. As regards genotypes, genotypes A and C are found in the samples.