Journal of School of Public Health and

Background and Aim: Iran National Polio Laboratory (NPL) is a member of the World Health Organization (WHO) Polio Laboratories Network. NPL receives stool specimens from acute flaccid paralysis (AFP) cases from all the provinces throughout Iran for poliovirus detection and identification. Furthermore, the NPL also detects non-polio enteroviruses (NPEVs) in these specimens.  Recently, NPEVs have come to be believed to be one of the most important causes of AFP following wild poliovirus.  This paper reports the prevalence of different types of NPEVs isolated from the specimens of AFP cases between 1995 and 2000.

Materials and Methods: Stool collection, virus detection and serotype identification were performed according to the WHO standard procedures.

Results: A total of 2180 stool specimens from AFP cases were received at the National Polio Laboratory. Coxsackie B viruse and echoviruses 6, 11, 7 and 13 had the highest frequency, identified in 23.7%, 14.4%, 12.7%, 11% and 10.2% of the NPEVs isolated from AFP cases, respectively. Four cases of echovirus 20 were identified, in 2 cases the patiets having died and in one the patient having been afflicted with residual paralysis. There have been no reports of death or residual paralysis (paralysis continuing after 60 days) due to echoviruse 20.

Conclusion:  Considering the upward trend of AFP cases in Iran, even after wild poliovirus eradication, studies are needed to determine the frequency and type identification of NPEVs and the relationship between NPEVs and residual paralysis in the post-eradication era (2000 onwards).

Background and Aim: Self-medication, as a common risky health behavior, has financial and non-financial costs for Iranians.  This study aimed at determining the situation of self-medication and factors influencing it among women in Yasuj, Iran.

Materials and Methods: This cross-sectional study was performed on 192 women referred to urban health care centers in Yasuj, selected randomly from the existing health files at the centers. Data were collected using a standard questionnaire based on the Health Belief Model and analyzed by SPSS 16 statistical software.

Results: The most widely used medicines for self-medication were those for common cold and antibiotics from among over-the-counter (OTC) drugs and prescription-only-medicines (POM), respectively. Perceived susceptibility (17.63± 3.46) was the main construct associated with self-medication among women.

Conclusion: Once the causes of self-medication and its relationship with the Health Belief Model constructs are known, designing and implementing educational programs aiming at promoting awareness and perception of population groups, especially women, seems necessary.

Background and Aim: Chlorpyrifos is an organophosphate pesticide used in agriculture for pest control. It is a relatively persistent poison and an environmental pollutant with adverse effects on human health. This study was conducted to isolate and characterize chlorpyrifos-degrading bacteria from rice field soils in Amol City, Iran.

Materials and Methods: In this experimental study, soil samples were collected from rice fields with a history of toxic pollution. A minimal salt broth (Msb) medium containing 100 mg/l chlorpyrifos as the carbon and energy source was used for isolating pesticide-degrading bacteria. The colonies were characterized by Gram staining and biochemical tests and sequencing was done using the PCR method.

Results: Four chlorpyrifos-degrading bacterial strains were isolated from the soils. They included Bacillus licheniformis strain IARI-M-12, Bacillus pumilus strain MS42, Bacillus cereus strain ESB15, and Delftia tsuruhatensis strain SJ113. The effects of temperature and pH on the bacterial strains were investigated. The Bacillus strains showed the fastest growth at a temperature of 35^o C and a pH=7 in a Msb medium containing chlorpyrifos.

Conclusion: The results of this study demonstrate that bacteria in the farmland can degrade the chlorpyrifos poison. Thus, these bacteria can be used to reduce the environmental problems resulting from soil contamination with chlorpyrifos in the ecosystem.